Cargando…
Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel
Implantable devices fabricated from austenitic type 316L stainless steel have been employed significantly in medicine, principally because the material displays excellent mechanical characteristics and corrosion resistance. It is well known, however, that interaction of exposure of such a material t...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125229/ https://www.ncbi.nlm.nih.gov/pubmed/33946387 http://dx.doi.org/10.3390/ma14092342 |
_version_ | 1783693439366332416 |
---|---|
author | Yang, Tairan De La Franier, Brian Thompson, Michael |
author_facet | Yang, Tairan De La Franier, Brian Thompson, Michael |
author_sort | Yang, Tairan |
collection | PubMed |
description | Implantable devices fabricated from austenitic type 316L stainless steel have been employed significantly in medicine, principally because the material displays excellent mechanical characteristics and corrosion resistance. It is well known, however, that interaction of exposure of such a material to blood can initiate platelet adhesion and blood coagulation, leading to a harmful medical condition. In order to prevent undesirable surface platelet adhesion on biomaterials employed in procedures such as renal dialysis, we developed an ultrathin anti-thrombogenic covalently attached monolayer based on monoethylene glycol silane chemistry. This functions by forming an interstitial hydration layer which displays restricted mobility in the prevention of surface fouling. In the present work, the promising anti-thrombogenic properties of this film are examined with respect to platelet aggregation on 316L austenitic stainless steel exposed to whole human blood. Prior to exposure with blood, all major surface modification steps were examined by X-ray photoelectron spectroscopic analysis and surface free-angle measurement by contact angle goniometry. End-stage anti-thrombogenicity detection after 20 min of blood exposure at 100 s(−1), 300 s(−1), 600 s(−1), 750 s(−1), and 900 s(−1) shear rates revealed that a significant reduction (>90%) of platelet adhesion and aggregation was achieved for surface-modified steel, compared with untreated material. This result is confirmed by experiments conducted in real time for 60-minute exposure to blood at 100 s(−1), 600 s(−1), and 900 s(−1) shear rates. |
format | Online Article Text |
id | pubmed-8125229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81252292021-05-17 Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel Yang, Tairan De La Franier, Brian Thompson, Michael Materials (Basel) Article Implantable devices fabricated from austenitic type 316L stainless steel have been employed significantly in medicine, principally because the material displays excellent mechanical characteristics and corrosion resistance. It is well known, however, that interaction of exposure of such a material to blood can initiate platelet adhesion and blood coagulation, leading to a harmful medical condition. In order to prevent undesirable surface platelet adhesion on biomaterials employed in procedures such as renal dialysis, we developed an ultrathin anti-thrombogenic covalently attached monolayer based on monoethylene glycol silane chemistry. This functions by forming an interstitial hydration layer which displays restricted mobility in the prevention of surface fouling. In the present work, the promising anti-thrombogenic properties of this film are examined with respect to platelet aggregation on 316L austenitic stainless steel exposed to whole human blood. Prior to exposure with blood, all major surface modification steps were examined by X-ray photoelectron spectroscopic analysis and surface free-angle measurement by contact angle goniometry. End-stage anti-thrombogenicity detection after 20 min of blood exposure at 100 s(−1), 300 s(−1), 600 s(−1), 750 s(−1), and 900 s(−1) shear rates revealed that a significant reduction (>90%) of platelet adhesion and aggregation was achieved for surface-modified steel, compared with untreated material. This result is confirmed by experiments conducted in real time for 60-minute exposure to blood at 100 s(−1), 600 s(−1), and 900 s(−1) shear rates. MDPI 2021-04-30 /pmc/articles/PMC8125229/ /pubmed/33946387 http://dx.doi.org/10.3390/ma14092342 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Tairan De La Franier, Brian Thompson, Michael Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel |
title | Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel |
title_full | Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel |
title_fullStr | Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel |
title_full_unstemmed | Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel |
title_short | Anti-Thrombogenicity Study of a Covalently-Attached Monolayer on Stent-Grade Stainless Steel |
title_sort | anti-thrombogenicity study of a covalently-attached monolayer on stent-grade stainless steel |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125229/ https://www.ncbi.nlm.nih.gov/pubmed/33946387 http://dx.doi.org/10.3390/ma14092342 |
work_keys_str_mv | AT yangtairan antithrombogenicitystudyofacovalentlyattachedmonolayeronstentgradestainlesssteel AT delafranierbrian antithrombogenicitystudyofacovalentlyattachedmonolayeronstentgradestainlesssteel AT thompsonmichael antithrombogenicitystudyofacovalentlyattachedmonolayeronstentgradestainlesssteel |