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A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft
This study aimed to examine the optimal cross-link density of recombinant peptide (RCP) particles, based on human collagen type I, for bone reconstruction in human alveolar cleft. Low- (group 1), medium- (group 2), and high- (group 3) cross-linked RCP particles were prepared by altering the duration...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125289/ https://www.ncbi.nlm.nih.gov/pubmed/33946797 http://dx.doi.org/10.3390/ma14092306 |
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author | Ito, Masaaki Toriumi, Taku Hiratsuka, Takahiro Imura, Hideto Akiyama, Yasunori Chimedtseren, Ichinnorov Arai, Yoshinori Yamaguchi, Kazuhiro Azuma, Akihiko Hata, Ken-ichiro Natsume, Nagato Honda, Masaki |
author_facet | Ito, Masaaki Toriumi, Taku Hiratsuka, Takahiro Imura, Hideto Akiyama, Yasunori Chimedtseren, Ichinnorov Arai, Yoshinori Yamaguchi, Kazuhiro Azuma, Akihiko Hata, Ken-ichiro Natsume, Nagato Honda, Masaki |
author_sort | Ito, Masaaki |
collection | PubMed |
description | This study aimed to examine the optimal cross-link density of recombinant peptide (RCP) particles, based on human collagen type I, for bone reconstruction in human alveolar cleft. Low- (group 1), medium- (group 2), and high- (group 3) cross-linked RCP particles were prepared by altering the duration of the heat-dependent dehydration reaction. Rat palatine fissures (n = 45), analogous to human congenital bone defects, were examined to evaluate the potential of bone formation by the three different RCP particles. Microcomputed tomography images were obtained to measure bone volume and bone mineral density at 4, 8, 12, and 16 weeks post grafting. Specimens were obtained for histological analysis at 16 weeks after grafting. Additionally, alkaline phosphatase and tartrate acid phosphatase staining were performed to visualize the presence of osteoblasts and osteoclasts. At 16 weeks, bone volume, bone mineral density, and new bone area measurements in group 2 were significantly higher than in any other group. In addition, the number of osteoblasts and osteoclasts on the new bone surface in group 2 was significantly higher than in any other group. Our results demonstrated that medium cross-linking was more suitable for bone formation—and could be useful in human alveolar cleft repairs as well. |
format | Online Article Text |
id | pubmed-8125289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-81252892021-05-17 A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft Ito, Masaaki Toriumi, Taku Hiratsuka, Takahiro Imura, Hideto Akiyama, Yasunori Chimedtseren, Ichinnorov Arai, Yoshinori Yamaguchi, Kazuhiro Azuma, Akihiko Hata, Ken-ichiro Natsume, Nagato Honda, Masaki Materials (Basel) Article This study aimed to examine the optimal cross-link density of recombinant peptide (RCP) particles, based on human collagen type I, for bone reconstruction in human alveolar cleft. Low- (group 1), medium- (group 2), and high- (group 3) cross-linked RCP particles were prepared by altering the duration of the heat-dependent dehydration reaction. Rat palatine fissures (n = 45), analogous to human congenital bone defects, were examined to evaluate the potential of bone formation by the three different RCP particles. Microcomputed tomography images were obtained to measure bone volume and bone mineral density at 4, 8, 12, and 16 weeks post grafting. Specimens were obtained for histological analysis at 16 weeks after grafting. Additionally, alkaline phosphatase and tartrate acid phosphatase staining were performed to visualize the presence of osteoblasts and osteoclasts. At 16 weeks, bone volume, bone mineral density, and new bone area measurements in group 2 were significantly higher than in any other group. In addition, the number of osteoblasts and osteoclasts on the new bone surface in group 2 was significantly higher than in any other group. Our results demonstrated that medium cross-linking was more suitable for bone formation—and could be useful in human alveolar cleft repairs as well. MDPI 2021-04-29 /pmc/articles/PMC8125289/ /pubmed/33946797 http://dx.doi.org/10.3390/ma14092306 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ito, Masaaki Toriumi, Taku Hiratsuka, Takahiro Imura, Hideto Akiyama, Yasunori Chimedtseren, Ichinnorov Arai, Yoshinori Yamaguchi, Kazuhiro Azuma, Akihiko Hata, Ken-ichiro Natsume, Nagato Honda, Masaki A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft |
title | A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft |
title_full | A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft |
title_fullStr | A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft |
title_full_unstemmed | A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft |
title_short | A Novel Bone Substitute Based on Recombinant Type I Collagen for Reconstruction of Alveolar Cleft |
title_sort | novel bone substitute based on recombinant type i collagen for reconstruction of alveolar cleft |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125289/ https://www.ncbi.nlm.nih.gov/pubmed/33946797 http://dx.doi.org/10.3390/ma14092306 |
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