Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles

Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and...

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Autores principales: Dai, Michèle, Georgilis, Evangelos, Goudounet, Guillaume, Garbay, Bertrand, Pille, Jan, van Hest, Jan C. M., Schultze, Xavier, Garanger, Elisabeth, Lecommandoux, Sébastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125372/
https://www.ncbi.nlm.nih.gov/pubmed/34062852
http://dx.doi.org/10.3390/polym13091470
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author Dai, Michèle
Georgilis, Evangelos
Goudounet, Guillaume
Garbay, Bertrand
Pille, Jan
van Hest, Jan C. M.
Schultze, Xavier
Garanger, Elisabeth
Lecommandoux, Sébastien
author_facet Dai, Michèle
Georgilis, Evangelos
Goudounet, Guillaume
Garbay, Bertrand
Pille, Jan
van Hest, Jan C. M.
Schultze, Xavier
Garanger, Elisabeth
Lecommandoux, Sébastien
author_sort Dai, Michèle
collection PubMed
description Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[M(1)V(3)-i]-[I-j]. First, ELP-[M(1)V(3)-i]-[I-j] (i = 20, 40, 60; j = 20, 90) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[M(1)V(3)-i] and ELP-[I-j]. By selectively oxidizing methionines of ELP-[M(1)V(3)-i] within the different diblocks structures, we have been able to access a thermal phase transition with three distinct regimes (unimers, micelles, aggregates) characteristic of well-defined ELP diblocks.
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spelling pubmed-81253722021-05-17 Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles Dai, Michèle Georgilis, Evangelos Goudounet, Guillaume Garbay, Bertrand Pille, Jan van Hest, Jan C. M. Schultze, Xavier Garanger, Elisabeth Lecommandoux, Sébastien Polymers (Basel) Article Diblock copolymers based-on elastin-like polypeptide (ELP) have the potential to undergo specific phase transitions when thermally stimulated. This ability is especially suitable to form carriers, micellar structures for instance, for delivering active cargo molecules. Here, we report the design and study of an ELP diblock library based on ELP-[M(1)V(3)-i]-[I-j]. First, ELP-[M(1)V(3)-i]-[I-j] (i = 20, 40, 60; j = 20, 90) that showed a similar self-assembly propensity (unimer-to-aggregate transition) as their related monoblocks ELP-[M(1)V(3)-i] and ELP-[I-j]. By selectively oxidizing methionines of ELP-[M(1)V(3)-i] within the different diblocks structures, we have been able to access a thermal phase transition with three distinct regimes (unimers, micelles, aggregates) characteristic of well-defined ELP diblocks. MDPI 2021-05-01 /pmc/articles/PMC8125372/ /pubmed/34062852 http://dx.doi.org/10.3390/polym13091470 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dai, Michèle
Georgilis, Evangelos
Goudounet, Guillaume
Garbay, Bertrand
Pille, Jan
van Hest, Jan C. M.
Schultze, Xavier
Garanger, Elisabeth
Lecommandoux, Sébastien
Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles
title Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles
title_full Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles
title_fullStr Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles
title_full_unstemmed Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles
title_short Refining the Design of Diblock Elastin-Like Polypeptides for Self-Assembly into Nanoparticles
title_sort refining the design of diblock elastin-like polypeptides for self-assembly into nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125372/
https://www.ncbi.nlm.nih.gov/pubmed/34062852
http://dx.doi.org/10.3390/polym13091470
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