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PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition

Advanced-stage oral cancers with lymph node metastasis are associated with poor prognosis and a high mortality rate. Although recent advancement in cancer treatment has effectively improved the oral cancer prognosis, the majority of therapeutic interventions are highly expensive and are associated w...

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Autores principales: Bharath Kumar, V., Lin, Jen-Tsun, Mahalakshmi, B., Chuang, Yi-Ching, Ho, Hsin-Yu, Lin, Chia-Chieh, Lo, Yu-Sheng, Hsieh, Ming-Ju, Chen, Mu-Kuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125947/
https://www.ncbi.nlm.nih.gov/pubmed/34065077
http://dx.doi.org/10.3390/ijms22095012
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author Bharath Kumar, V.
Lin, Jen-Tsun
Mahalakshmi, B.
Chuang, Yi-Ching
Ho, Hsin-Yu
Lin, Chia-Chieh
Lo, Yu-Sheng
Hsieh, Ming-Ju
Chen, Mu-Kuan
author_facet Bharath Kumar, V.
Lin, Jen-Tsun
Mahalakshmi, B.
Chuang, Yi-Ching
Ho, Hsin-Yu
Lin, Chia-Chieh
Lo, Yu-Sheng
Hsieh, Ming-Ju
Chen, Mu-Kuan
author_sort Bharath Kumar, V.
collection PubMed
description Advanced-stage oral cancers with lymph node metastasis are associated with poor prognosis and a high mortality rate. Although recent advancement in cancer treatment has effectively improved the oral cancer prognosis, the majority of therapeutic interventions are highly expensive and are associated with severe sideeffects. In the present study, we studied the efficacy of a diarylheptanoid derivative, platyphyllenone, in modulating the metastatic potential of human oral cancer cells. Specifically, we treated the human oral cancer cells (FaDu, Ca9-22, and HSC3) with different concentrations of platyphyllenone and measured the cell proliferation, migration, and invasion. The study findings revealed that platyphyllenonesignificantly inhibited the motility, migration, and invasion of human oral cancer cells. Mechanistically, platyphyllenone reduced p38 phosphorylation, decreased β-catenin and Slug, increased E-cadherin expression, and reduced cathepsin L expression, which collectively led to a reduction in cancer cell migration and invasion. Taken together, our study indicates that platyphyllenone exerts significant anti-metastatic effects on oral cancer cells by modulating cathepsin L expression, the MAPK signaling pathway, and the epithelial–mesenchymal transition process.
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spelling pubmed-81259472021-05-17 PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition Bharath Kumar, V. Lin, Jen-Tsun Mahalakshmi, B. Chuang, Yi-Ching Ho, Hsin-Yu Lin, Chia-Chieh Lo, Yu-Sheng Hsieh, Ming-Ju Chen, Mu-Kuan Int J Mol Sci Article Advanced-stage oral cancers with lymph node metastasis are associated with poor prognosis and a high mortality rate. Although recent advancement in cancer treatment has effectively improved the oral cancer prognosis, the majority of therapeutic interventions are highly expensive and are associated with severe sideeffects. In the present study, we studied the efficacy of a diarylheptanoid derivative, platyphyllenone, in modulating the metastatic potential of human oral cancer cells. Specifically, we treated the human oral cancer cells (FaDu, Ca9-22, and HSC3) with different concentrations of platyphyllenone and measured the cell proliferation, migration, and invasion. The study findings revealed that platyphyllenonesignificantly inhibited the motility, migration, and invasion of human oral cancer cells. Mechanistically, platyphyllenone reduced p38 phosphorylation, decreased β-catenin and Slug, increased E-cadherin expression, and reduced cathepsin L expression, which collectively led to a reduction in cancer cell migration and invasion. Taken together, our study indicates that platyphyllenone exerts significant anti-metastatic effects on oral cancer cells by modulating cathepsin L expression, the MAPK signaling pathway, and the epithelial–mesenchymal transition process. MDPI 2021-05-09 /pmc/articles/PMC8125947/ /pubmed/34065077 http://dx.doi.org/10.3390/ijms22095012 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bharath Kumar, V.
Lin, Jen-Tsun
Mahalakshmi, B.
Chuang, Yi-Ching
Ho, Hsin-Yu
Lin, Chia-Chieh
Lo, Yu-Sheng
Hsieh, Ming-Ju
Chen, Mu-Kuan
PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition
title PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition
title_full PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition
title_fullStr PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition
title_full_unstemmed PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition
title_short PlatyphyllenoneExerts Anti-Metastatic Effects on Human Oral Cancer Cells by Modulating Cathepsin L Expression, MAPK Pathway and Epithelial–Mesenchymal Transition
title_sort platyphyllenoneexerts anti-metastatic effects on human oral cancer cells by modulating cathepsin l expression, mapk pathway and epithelial–mesenchymal transition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8125947/
https://www.ncbi.nlm.nih.gov/pubmed/34065077
http://dx.doi.org/10.3390/ijms22095012
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