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Ustilago maydis effector Jsi1 interacts with Topless corepressor, hijacking plant jasmonate/ethylene signaling

Ustilago maydis is the causal agent of maize smut disease. During the colonization process, the fungus secretes effector proteins that suppress immune responses and redirect the host metabolism in favor of the pathogen. As effectors play a critical role during plant colonization, their identificatio...

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Autores principales: Darino, Martin, Chia, Khong‐Sam, Marques, Joana, Aleksza, David, Soto‐Jiménez, Luz Mayela, Saado, Indira, Uhse, Simon, Borg, Michael, Betz, Ruben, Bindics, Janos, Zienkiewicz, Krzysztof, Feussner, Ivo, Petit‐Houdenot, Yohann, Djamei, Armin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126959/
https://www.ncbi.nlm.nih.gov/pubmed/33247447
http://dx.doi.org/10.1111/nph.17116
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author Darino, Martin
Chia, Khong‐Sam
Marques, Joana
Aleksza, David
Soto‐Jiménez, Luz Mayela
Saado, Indira
Uhse, Simon
Borg, Michael
Betz, Ruben
Bindics, Janos
Zienkiewicz, Krzysztof
Feussner, Ivo
Petit‐Houdenot, Yohann
Djamei, Armin
author_facet Darino, Martin
Chia, Khong‐Sam
Marques, Joana
Aleksza, David
Soto‐Jiménez, Luz Mayela
Saado, Indira
Uhse, Simon
Borg, Michael
Betz, Ruben
Bindics, Janos
Zienkiewicz, Krzysztof
Feussner, Ivo
Petit‐Houdenot, Yohann
Djamei, Armin
author_sort Darino, Martin
collection PubMed
description Ustilago maydis is the causal agent of maize smut disease. During the colonization process, the fungus secretes effector proteins that suppress immune responses and redirect the host metabolism in favor of the pathogen. As effectors play a critical role during plant colonization, their identification and functional characterization are essential to understanding biotrophy and disease. Using biochemical, molecular, and transcriptomic techniques, we performed a functional characterization of the U. maydis effector Jasmonate/Ethylene signaling inducer 1 (Jsi1). Jsi1 interacts with several members of the plant corepressor family Topless/Topless related (TPL/TPR). Jsi1 expression in Zea mays and Arabidopsis thaliana leads to transcriptional induction of the ethylene response factor (ERF) branch of the jasmonate/ethylene (JA/ET) signaling pathway. In A. thaliana, activation of the ERF branch leads to biotrophic susceptibility. Jsi1 likely activates the ERF branch via an EAR (ET‐responsive element binding‐factor‐associated amphiphilic repression) motif, which resembles EAR motifs from plant ERF transcription factors, that interacts with TPL/TPR proteins. EAR‐motif‐containing effector candidates were identified from different fungal species, including Magnaporthe oryzae, Sporisorium scitamineum, and Sporisorium reilianum. Interaction between plant TPL proteins and these effector candidates from biotrophic and hemibiotrophic fungi indicates the convergent evolution of effectors modulating the TPL/TPR corepressor hub.
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spelling pubmed-81269592021-05-21 Ustilago maydis effector Jsi1 interacts with Topless corepressor, hijacking plant jasmonate/ethylene signaling Darino, Martin Chia, Khong‐Sam Marques, Joana Aleksza, David Soto‐Jiménez, Luz Mayela Saado, Indira Uhse, Simon Borg, Michael Betz, Ruben Bindics, Janos Zienkiewicz, Krzysztof Feussner, Ivo Petit‐Houdenot, Yohann Djamei, Armin New Phytol Research Ustilago maydis is the causal agent of maize smut disease. During the colonization process, the fungus secretes effector proteins that suppress immune responses and redirect the host metabolism in favor of the pathogen. As effectors play a critical role during plant colonization, their identification and functional characterization are essential to understanding biotrophy and disease. Using biochemical, molecular, and transcriptomic techniques, we performed a functional characterization of the U. maydis effector Jasmonate/Ethylene signaling inducer 1 (Jsi1). Jsi1 interacts with several members of the plant corepressor family Topless/Topless related (TPL/TPR). Jsi1 expression in Zea mays and Arabidopsis thaliana leads to transcriptional induction of the ethylene response factor (ERF) branch of the jasmonate/ethylene (JA/ET) signaling pathway. In A. thaliana, activation of the ERF branch leads to biotrophic susceptibility. Jsi1 likely activates the ERF branch via an EAR (ET‐responsive element binding‐factor‐associated amphiphilic repression) motif, which resembles EAR motifs from plant ERF transcription factors, that interacts with TPL/TPR proteins. EAR‐motif‐containing effector candidates were identified from different fungal species, including Magnaporthe oryzae, Sporisorium scitamineum, and Sporisorium reilianum. Interaction between plant TPL proteins and these effector candidates from biotrophic and hemibiotrophic fungi indicates the convergent evolution of effectors modulating the TPL/TPR corepressor hub. John Wiley and Sons Inc. 2021-01-03 2021-03 /pmc/articles/PMC8126959/ /pubmed/33247447 http://dx.doi.org/10.1111/nph.17116 Text en © 2020 The Authors. New Phytologist © 2020 New Phytologist Foundation https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Darino, Martin
Chia, Khong‐Sam
Marques, Joana
Aleksza, David
Soto‐Jiménez, Luz Mayela
Saado, Indira
Uhse, Simon
Borg, Michael
Betz, Ruben
Bindics, Janos
Zienkiewicz, Krzysztof
Feussner, Ivo
Petit‐Houdenot, Yohann
Djamei, Armin
Ustilago maydis effector Jsi1 interacts with Topless corepressor, hijacking plant jasmonate/ethylene signaling
title Ustilago maydis effector Jsi1 interacts with Topless corepressor, hijacking plant jasmonate/ethylene signaling
title_full Ustilago maydis effector Jsi1 interacts with Topless corepressor, hijacking plant jasmonate/ethylene signaling
title_fullStr Ustilago maydis effector Jsi1 interacts with Topless corepressor, hijacking plant jasmonate/ethylene signaling
title_full_unstemmed Ustilago maydis effector Jsi1 interacts with Topless corepressor, hijacking plant jasmonate/ethylene signaling
title_short Ustilago maydis effector Jsi1 interacts with Topless corepressor, hijacking plant jasmonate/ethylene signaling
title_sort ustilago maydis effector jsi1 interacts with topless corepressor, hijacking plant jasmonate/ethylene signaling
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8126959/
https://www.ncbi.nlm.nih.gov/pubmed/33247447
http://dx.doi.org/10.1111/nph.17116
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