A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues

Though much of the interest in fluorescence in the past has been on measuring spectral qualities such as wavelength and intensity, there are two other highly useful intrinsic properties of fluorescence: lifetime (or decay) and anisotropy (or polarization). Each has its own set of unique advantages,...

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Autores principales: Bitton, Aric, Sambrano, Jesus, Valentino, Samantha, Houston, Jessica P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127321/
https://www.ncbi.nlm.nih.gov/pubmed/34007839
http://dx.doi.org/10.3389/fphy.2021.648553
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author Bitton, Aric
Sambrano, Jesus
Valentino, Samantha
Houston, Jessica P.
author_facet Bitton, Aric
Sambrano, Jesus
Valentino, Samantha
Houston, Jessica P.
author_sort Bitton, Aric
collection PubMed
description Though much of the interest in fluorescence in the past has been on measuring spectral qualities such as wavelength and intensity, there are two other highly useful intrinsic properties of fluorescence: lifetime (or decay) and anisotropy (or polarization). Each has its own set of unique advantages, limitations, and challenges in detection when it comes to use in biological studies. This review will focus on the property of fluorescence lifetime, providing a brief background on instrumentation and theory, and examine the recent advancements and applications of measuring lifetime in the fields of high-throughput fluorescence lifetime imaging microscopy (HT-FLIM) and time-resolved flow cytometry (TRFC). In addition, the crossover of these two methods and their outlooks will be discussed.
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spelling pubmed-81273212021-05-17 A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues Bitton, Aric Sambrano, Jesus Valentino, Samantha Houston, Jessica P. Front Phys Article Though much of the interest in fluorescence in the past has been on measuring spectral qualities such as wavelength and intensity, there are two other highly useful intrinsic properties of fluorescence: lifetime (or decay) and anisotropy (or polarization). Each has its own set of unique advantages, limitations, and challenges in detection when it comes to use in biological studies. This review will focus on the property of fluorescence lifetime, providing a brief background on instrumentation and theory, and examine the recent advancements and applications of measuring lifetime in the fields of high-throughput fluorescence lifetime imaging microscopy (HT-FLIM) and time-resolved flow cytometry (TRFC). In addition, the crossover of these two methods and their outlooks will be discussed. 2021-04-26 2021-04 /pmc/articles/PMC8127321/ /pubmed/34007839 http://dx.doi.org/10.3389/fphy.2021.648553 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Article
Bitton, Aric
Sambrano, Jesus
Valentino, Samantha
Houston, Jessica P.
A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues
title A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues
title_full A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues
title_fullStr A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues
title_full_unstemmed A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues
title_short A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues
title_sort review of new high-throughput methods designed for fluorescence lifetime sensing from cells and tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127321/
https://www.ncbi.nlm.nih.gov/pubmed/34007839
http://dx.doi.org/10.3389/fphy.2021.648553
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