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Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA
INTRODUCTION: SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca(2+)) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR). METHODS: Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lava...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127738/ https://www.ncbi.nlm.nih.gov/pubmed/33983057 http://dx.doi.org/10.1177/20587384211015054 |
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author | Ba, Guangyi Tang, Ru Sun, Xiwen Li, Zhipeng Lin, Hai Zhang, Weitian |
author_facet | Ba, Guangyi Tang, Ru Sun, Xiwen Li, Zhipeng Lin, Hai Zhang, Weitian |
author_sort | Ba, Guangyi |
collection | PubMed |
description | INTRODUCTION: SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca(2+)) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR). METHODS: Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lavage fluid (NLF) from mice were harvested to assay IgE and inflammatory cytokines using ELISA method. Inflammatory cells were counted and analyzed in NLF. Nasal mucosa tissues were collected from mice and used for HE staining, immunohistochemistry (IHC) staining, and real-time PCR detection. RESULTS: SKF-96365 had therapeutic effects on murine AR manifesting attenuation of sneezing, nasal rubbing, IgE, inflammatory cytokines, inflammatory cells, TRPC6 immunolabeling, and TRPC6, STIM1 and Orai1 mRNA levels in AR mice. CONCLUSION: SKF-96365 could effectively alleviate the symptoms of murine AR. SKF-96365 could suppress TRPC6, STIM1, and Orai1 activities, leading to the downregulation of inflammatory cytokines and inflammatory cells in murine AR. |
format | Online Article Text |
id | pubmed-8127738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-81277382021-05-24 Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA Ba, Guangyi Tang, Ru Sun, Xiwen Li, Zhipeng Lin, Hai Zhang, Weitian Int J Immunopathol Pharmacol Original Research Article INTRODUCTION: SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca(2+)) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR). METHODS: Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lavage fluid (NLF) from mice were harvested to assay IgE and inflammatory cytokines using ELISA method. Inflammatory cells were counted and analyzed in NLF. Nasal mucosa tissues were collected from mice and used for HE staining, immunohistochemistry (IHC) staining, and real-time PCR detection. RESULTS: SKF-96365 had therapeutic effects on murine AR manifesting attenuation of sneezing, nasal rubbing, IgE, inflammatory cytokines, inflammatory cells, TRPC6 immunolabeling, and TRPC6, STIM1 and Orai1 mRNA levels in AR mice. CONCLUSION: SKF-96365 could effectively alleviate the symptoms of murine AR. SKF-96365 could suppress TRPC6, STIM1, and Orai1 activities, leading to the downregulation of inflammatory cytokines and inflammatory cells in murine AR. SAGE Publications 2021-05-13 /pmc/articles/PMC8127738/ /pubmed/33983057 http://dx.doi.org/10.1177/20587384211015054 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Article Ba, Guangyi Tang, Ru Sun, Xiwen Li, Zhipeng Lin, Hai Zhang, Weitian Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA |
title | Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA |
title_full | Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA |
title_fullStr | Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA |
title_full_unstemmed | Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA |
title_short | Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA |
title_sort | therapeutic effects of skf-96365 on murine allergic rhinitis induced by ova |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127738/ https://www.ncbi.nlm.nih.gov/pubmed/33983057 http://dx.doi.org/10.1177/20587384211015054 |
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