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Long noncoding RNA LINC01296 plays an oncogenic role in colorectal cancer by suppressing p15 expression

OBJECTIVE: To examine the role of the long noncoding RNA LINC01296 in colorectal carcinoma (CRC) and to explore the underlying mechanism. METHODS: We detected LINC01296 expression levels in a cohort of 51 paired CRC and normal tissues. We also assessed the effects of LINC01296 on cell proliferation...

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Autores principales: Xu, Jianing, Zhang, Zhehao, Shen, Dong, Zhang, Ting, Zhang, Jinsong, De, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127761/
https://www.ncbi.nlm.nih.gov/pubmed/33983053
http://dx.doi.org/10.1177/03000605211004414
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author Xu, Jianing
Zhang, Zhehao
Shen, Dong
Zhang, Ting
Zhang, Jinsong
De, Wei
author_facet Xu, Jianing
Zhang, Zhehao
Shen, Dong
Zhang, Ting
Zhang, Jinsong
De, Wei
author_sort Xu, Jianing
collection PubMed
description OBJECTIVE: To examine the role of the long noncoding RNA LINC01296 in colorectal carcinoma (CRC) and to explore the underlying mechanism. METHODS: We detected LINC01296 expression levels in a cohort of 51 paired CRC and normal tissues. We also assessed the effects of LINC01296 on cell proliferation and apoptosis in CRC cells in vitro, and measured its effect on tumor growth in an in vivo mouse model. We identified the potential downstream targets of LINC01296 and assessed its regulatory effects. RESULTS: Expression levels of LINC01296 were elevated in 37/51 CRC tissues compared with the corresponding normal tissues and were significantly associated with tumor stage, lymph node metastasis, and distant metastasis. Knockdown of LINC01296 using antisense oligonucleotides inhibited cell proliferation and promoted apoptosis of colon cancer cells in vitro and inhibited tumor growth in vivo. Knockdown of LINC01296 also significantly increased the gene expression of p15 in colon cancer cells. LINC01296-specific suppression of p15 was validated by the interaction between enhancer of zeste homolog 2 and LINC01296. CONCLUSION: Overexpression of LINC01296 suppressed the expression of p15 leading to CRC carcinogenesis. These findings may provide the basis for novel future CRC-targeted therapies.
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spelling pubmed-81277612021-05-24 Long noncoding RNA LINC01296 plays an oncogenic role in colorectal cancer by suppressing p15 expression Xu, Jianing Zhang, Zhehao Shen, Dong Zhang, Ting Zhang, Jinsong De, Wei J Int Med Res Retrospective Clinical Research Report OBJECTIVE: To examine the role of the long noncoding RNA LINC01296 in colorectal carcinoma (CRC) and to explore the underlying mechanism. METHODS: We detected LINC01296 expression levels in a cohort of 51 paired CRC and normal tissues. We also assessed the effects of LINC01296 on cell proliferation and apoptosis in CRC cells in vitro, and measured its effect on tumor growth in an in vivo mouse model. We identified the potential downstream targets of LINC01296 and assessed its regulatory effects. RESULTS: Expression levels of LINC01296 were elevated in 37/51 CRC tissues compared with the corresponding normal tissues and were significantly associated with tumor stage, lymph node metastasis, and distant metastasis. Knockdown of LINC01296 using antisense oligonucleotides inhibited cell proliferation and promoted apoptosis of colon cancer cells in vitro and inhibited tumor growth in vivo. Knockdown of LINC01296 also significantly increased the gene expression of p15 in colon cancer cells. LINC01296-specific suppression of p15 was validated by the interaction between enhancer of zeste homolog 2 and LINC01296. CONCLUSION: Overexpression of LINC01296 suppressed the expression of p15 leading to CRC carcinogenesis. These findings may provide the basis for novel future CRC-targeted therapies. SAGE Publications 2021-05-13 /pmc/articles/PMC8127761/ /pubmed/33983053 http://dx.doi.org/10.1177/03000605211004414 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Retrospective Clinical Research Report
Xu, Jianing
Zhang, Zhehao
Shen, Dong
Zhang, Ting
Zhang, Jinsong
De, Wei
Long noncoding RNA LINC01296 plays an oncogenic role in colorectal cancer by suppressing p15 expression
title Long noncoding RNA LINC01296 plays an oncogenic role in colorectal cancer by suppressing p15 expression
title_full Long noncoding RNA LINC01296 plays an oncogenic role in colorectal cancer by suppressing p15 expression
title_fullStr Long noncoding RNA LINC01296 plays an oncogenic role in colorectal cancer by suppressing p15 expression
title_full_unstemmed Long noncoding RNA LINC01296 plays an oncogenic role in colorectal cancer by suppressing p15 expression
title_short Long noncoding RNA LINC01296 plays an oncogenic role in colorectal cancer by suppressing p15 expression
title_sort long noncoding rna linc01296 plays an oncogenic role in colorectal cancer by suppressing p15 expression
topic Retrospective Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127761/
https://www.ncbi.nlm.nih.gov/pubmed/33983053
http://dx.doi.org/10.1177/03000605211004414
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