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An improved in vivo tethering assay with single molecule FISH reveals that a nematode Nanos enhances reporter expression and mRNA stability

Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein–mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the C. elegans adult germline. The assay relies on a dual repor...

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Detalles Bibliográficos
Autores principales: Doenier, Jonathan, Lynch, Tina R., Kimble, Judith, Aoki, Scott T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8127996/
https://www.ncbi.nlm.nih.gov/pubmed/33727224
http://dx.doi.org/10.1261/rna.078693.121