Cargando…
Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing
Testosterone and alendronate have been identified as two bone healing compounds which, when combined, synergistically stimulate bone regeneration. This study describes the development of a novel ultrasonic spray coating for sustained release of ancillary amounts of testosterone and alendronate encap...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128250/ https://www.ncbi.nlm.nih.gov/pubmed/33999955 http://dx.doi.org/10.1371/journal.pone.0251864 |
_version_ | 1783694083995205632 |
---|---|
author | van de Ven, Cindy J. J. M. Bakker, Nicole E. C. Link, Dennis P. Geven, Edwin J. W. Gossen, Jan A. |
author_facet | van de Ven, Cindy J. J. M. Bakker, Nicole E. C. Link, Dennis P. Geven, Edwin J. W. Gossen, Jan A. |
author_sort | van de Ven, Cindy J. J. M. |
collection | PubMed |
description | Testosterone and alendronate have been identified as two bone healing compounds which, when combined, synergistically stimulate bone regeneration. This study describes the development of a novel ultrasonic spray coating for sustained release of ancillary amounts of testosterone and alendronate encapsulated in PLGA 5004A as a carrier. Due to the low amounts of testosterone and alendronate used, sensitive in vitro assays were developed to determine in vitro release. The ultrasonic spray coating technology was optimized for coating titanium screws and pericardial collagen membranes, with the aim to improve osseo-integration and (guided) bone regeneration, respectively, without interfering with their primary mode of action. In vitro release analysis of collagen membranes and screws showed up to 21 days sustained release of the compounds without a burst release. Subsequent preclinical studies in rat and rabbit models indicated that testosterone and alendronate coated membranes and screws significantly improved bone regeneration in vivo. Coated membranes significantly improved the formation of new bone in a critical size calvarial defect model in rats (by 160% compared to controls). Coated screws implanted in rabbit femoral condyles significantly improved bone implant contact (69% vs 54% in controls), bone mineral density (121%) and bone volume (119%) up to 1.3 mm from the implant. Based on the results obtained, we suggest that implants or membranes enabled with local sustained delivery of ancillary amounts of testosterone and alendronate can be a promising system to stimulate local bone regeneration resulting in improved osseo-integration of implants and improved healing of bone defects and fractures. |
format | Online Article Text |
id | pubmed-8128250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81282502021-05-27 Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing van de Ven, Cindy J. J. M. Bakker, Nicole E. C. Link, Dennis P. Geven, Edwin J. W. Gossen, Jan A. PLoS One Research Article Testosterone and alendronate have been identified as two bone healing compounds which, when combined, synergistically stimulate bone regeneration. This study describes the development of a novel ultrasonic spray coating for sustained release of ancillary amounts of testosterone and alendronate encapsulated in PLGA 5004A as a carrier. Due to the low amounts of testosterone and alendronate used, sensitive in vitro assays were developed to determine in vitro release. The ultrasonic spray coating technology was optimized for coating titanium screws and pericardial collagen membranes, with the aim to improve osseo-integration and (guided) bone regeneration, respectively, without interfering with their primary mode of action. In vitro release analysis of collagen membranes and screws showed up to 21 days sustained release of the compounds without a burst release. Subsequent preclinical studies in rat and rabbit models indicated that testosterone and alendronate coated membranes and screws significantly improved bone regeneration in vivo. Coated membranes significantly improved the formation of new bone in a critical size calvarial defect model in rats (by 160% compared to controls). Coated screws implanted in rabbit femoral condyles significantly improved bone implant contact (69% vs 54% in controls), bone mineral density (121%) and bone volume (119%) up to 1.3 mm from the implant. Based on the results obtained, we suggest that implants or membranes enabled with local sustained delivery of ancillary amounts of testosterone and alendronate can be a promising system to stimulate local bone regeneration resulting in improved osseo-integration of implants and improved healing of bone defects and fractures. Public Library of Science 2021-05-17 /pmc/articles/PMC8128250/ /pubmed/33999955 http://dx.doi.org/10.1371/journal.pone.0251864 Text en © 2021 van de Ven et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article van de Ven, Cindy J. J. M. Bakker, Nicole E. C. Link, Dennis P. Geven, Edwin J. W. Gossen, Jan A. Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing |
title | Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing |
title_full | Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing |
title_fullStr | Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing |
title_full_unstemmed | Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing |
title_short | Sustained release of ancillary amounts of testosterone and alendronate from PLGA coated pericard membranes and implants to improve bone healing |
title_sort | sustained release of ancillary amounts of testosterone and alendronate from plga coated pericard membranes and implants to improve bone healing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8128250/ https://www.ncbi.nlm.nih.gov/pubmed/33999955 http://dx.doi.org/10.1371/journal.pone.0251864 |
work_keys_str_mv | AT vandevencindyjjm sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing AT bakkernicoleec sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing AT linkdennisp sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing AT gevenedwinjw sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing AT gossenjana sustainedreleaseofancillaryamountsoftestosteroneandalendronatefromplgacoatedpericardmembranesandimplantstoimprovebonehealing |