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Integrating Cycled Enzymatic DNA Amplification and Surface-Enhanced Raman Scattering for Sensitive Detection of Circulating Tumor DNA

Circulating tumor DNA (ctDNA) represents an emerging biomarker of liquid biopsies for the development of precision cancer diagnostics and therapeutics. However, sensitive detection of ctDNA remains challenging, due to their short half-life and low concentrations in blood samples. In this study, we r...

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Autores principales: Miao, Xinxing, Fang, Qianqian, Xiao, Xiang, Liu, Sidi, Wu, Renfei, Yan, Jun, Nie, Baoqing, Liu, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129026/
https://www.ncbi.nlm.nih.gov/pubmed/34017856
http://dx.doi.org/10.3389/fmolb.2021.676065
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author Miao, Xinxing
Fang, Qianqian
Xiao, Xiang
Liu, Sidi
Wu, Renfei
Yan, Jun
Nie, Baoqing
Liu, Jian
author_facet Miao, Xinxing
Fang, Qianqian
Xiao, Xiang
Liu, Sidi
Wu, Renfei
Yan, Jun
Nie, Baoqing
Liu, Jian
author_sort Miao, Xinxing
collection PubMed
description Circulating tumor DNA (ctDNA) represents an emerging biomarker of liquid biopsies for the development of precision cancer diagnostics and therapeutics. However, sensitive detection of ctDNA remains challenging, due to their short half-life and low concentrations in blood samples. In this study, we report a new method to address this challenge by integrating cycled enzymatic DNA amplification technique and Au nanoparticle@silicon-assisted surface-enhanced Raman scattering (SERS) technique. We have demonstrated a reproducible identification of a single-base-mutated ctDNA sequence of diffuse intrinsic pontine gliomas (DIPGs), with the limit of detection (LOD) as low as 9.1 fM in the spiked blood samples. This approach can be used to analyze trace amounts of ctDNA in translational medicine for early diagnosis, therapeutic effect monitoring, and prognosis of patients with cancer.
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spelling pubmed-81290262021-05-19 Integrating Cycled Enzymatic DNA Amplification and Surface-Enhanced Raman Scattering for Sensitive Detection of Circulating Tumor DNA Miao, Xinxing Fang, Qianqian Xiao, Xiang Liu, Sidi Wu, Renfei Yan, Jun Nie, Baoqing Liu, Jian Front Mol Biosci Molecular Biosciences Circulating tumor DNA (ctDNA) represents an emerging biomarker of liquid biopsies for the development of precision cancer diagnostics and therapeutics. However, sensitive detection of ctDNA remains challenging, due to their short half-life and low concentrations in blood samples. In this study, we report a new method to address this challenge by integrating cycled enzymatic DNA amplification technique and Au nanoparticle@silicon-assisted surface-enhanced Raman scattering (SERS) technique. We have demonstrated a reproducible identification of a single-base-mutated ctDNA sequence of diffuse intrinsic pontine gliomas (DIPGs), with the limit of detection (LOD) as low as 9.1 fM in the spiked blood samples. This approach can be used to analyze trace amounts of ctDNA in translational medicine for early diagnosis, therapeutic effect monitoring, and prognosis of patients with cancer. Frontiers Media S.A. 2021-05-04 /pmc/articles/PMC8129026/ /pubmed/34017856 http://dx.doi.org/10.3389/fmolb.2021.676065 Text en Copyright © 2021 Miao, Fang, Xiao, Liu, Wu, Yan, Nie and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Miao, Xinxing
Fang, Qianqian
Xiao, Xiang
Liu, Sidi
Wu, Renfei
Yan, Jun
Nie, Baoqing
Liu, Jian
Integrating Cycled Enzymatic DNA Amplification and Surface-Enhanced Raman Scattering for Sensitive Detection of Circulating Tumor DNA
title Integrating Cycled Enzymatic DNA Amplification and Surface-Enhanced Raman Scattering for Sensitive Detection of Circulating Tumor DNA
title_full Integrating Cycled Enzymatic DNA Amplification and Surface-Enhanced Raman Scattering for Sensitive Detection of Circulating Tumor DNA
title_fullStr Integrating Cycled Enzymatic DNA Amplification and Surface-Enhanced Raman Scattering for Sensitive Detection of Circulating Tumor DNA
title_full_unstemmed Integrating Cycled Enzymatic DNA Amplification and Surface-Enhanced Raman Scattering for Sensitive Detection of Circulating Tumor DNA
title_short Integrating Cycled Enzymatic DNA Amplification and Surface-Enhanced Raman Scattering for Sensitive Detection of Circulating Tumor DNA
title_sort integrating cycled enzymatic dna amplification and surface-enhanced raman scattering for sensitive detection of circulating tumor dna
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8129026/
https://www.ncbi.nlm.nih.gov/pubmed/34017856
http://dx.doi.org/10.3389/fmolb.2021.676065
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