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Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus: An observational study

BACKGROUND: Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus (T1DM). However, the alterations in the gut microbiota and their correlation with fasting blood glucose (FBG) in Chinese children with T1DM remain unclear. AIM: To investigate...

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Autores principales: Liu, Xia, Cheng, Yi-Wen, Shao, Li, Sun, Shu-Hong, Wu, Jian, Song, Qing-Hai, Zou, Hong-Sheng, Ling, Zong-Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130045/
https://www.ncbi.nlm.nih.gov/pubmed/34040330
http://dx.doi.org/10.3748/wjg.v27.i19.2394
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author Liu, Xia
Cheng, Yi-Wen
Shao, Li
Sun, Shu-Hong
Wu, Jian
Song, Qing-Hai
Zou, Hong-Sheng
Ling, Zong-Xin
author_facet Liu, Xia
Cheng, Yi-Wen
Shao, Li
Sun, Shu-Hong
Wu, Jian
Song, Qing-Hai
Zou, Hong-Sheng
Ling, Zong-Xin
author_sort Liu, Xia
collection PubMed
description BACKGROUND: Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus (T1DM). However, the alterations in the gut microbiota and their correlation with fasting blood glucose (FBG) in Chinese children with T1DM remain unclear. AIM: To investigate alterations in the gut microbiota in Chinese children with T1DM and their associations with clinical indicators. METHODS: Samples from 51 children with T1DM and 47 age-matched and gender-matched healthy controls were obtained, to explore the structural and functional alterations in the fecal microbiota. The V3-V4 regions of the 16S rRNA gene were sequenced on a MiSeq instrument, and the association with FBG were analyzed. RESULTS: We found that the bacterial diversity was significantly increased in the T1DM-associated fecal microbiota, and changes in the microbial composition were observed at different taxonomic levels. The T1DM-reduced differential taxa, such as Bacteroides vulgatus ATCC8482, Bacteroides ovatus, Bacteroides xylanisolvens, and Flavonifractor plautii, were negatively correlated with FBG, while the T1DM-enriched taxa, such as Blautia, Eubacterium hallii group, Anaerostipes hadrus, and Dorea longicatena, were positively correlated with FBG. Bacteroides vulgatus ATCC8482, Bacteroides ovatus, the Eubacterium hallii group, and Anaerostipes hadrus, either alone or in combination, could be used as noninvasive diagnostic biomarkers to discriminate children with T1DM from healthy controls. In addition, the functional changes in the T1DM-associated fecal microbiota also suggest that these fecal microbes were associated with altered functions and metabolic activities, such as glycan biosynthesis and metabolism and lipid metabolism, which might play vital roles in the pathogenesis and development of T1DM. CONCLUSION: Our present comprehensive investigation of the T1DM-associated fecal microbiota provides novel insights into the pathogenesis of the disease and sheds light on the diagnosis and treatment of T1DM.
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spelling pubmed-81300452021-05-25 Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus: An observational study Liu, Xia Cheng, Yi-Wen Shao, Li Sun, Shu-Hong Wu, Jian Song, Qing-Hai Zou, Hong-Sheng Ling, Zong-Xin World J Gastroenterol Observational Study BACKGROUND: Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus (T1DM). However, the alterations in the gut microbiota and their correlation with fasting blood glucose (FBG) in Chinese children with T1DM remain unclear. AIM: To investigate alterations in the gut microbiota in Chinese children with T1DM and their associations with clinical indicators. METHODS: Samples from 51 children with T1DM and 47 age-matched and gender-matched healthy controls were obtained, to explore the structural and functional alterations in the fecal microbiota. The V3-V4 regions of the 16S rRNA gene were sequenced on a MiSeq instrument, and the association with FBG were analyzed. RESULTS: We found that the bacterial diversity was significantly increased in the T1DM-associated fecal microbiota, and changes in the microbial composition were observed at different taxonomic levels. The T1DM-reduced differential taxa, such as Bacteroides vulgatus ATCC8482, Bacteroides ovatus, Bacteroides xylanisolvens, and Flavonifractor plautii, were negatively correlated with FBG, while the T1DM-enriched taxa, such as Blautia, Eubacterium hallii group, Anaerostipes hadrus, and Dorea longicatena, were positively correlated with FBG. Bacteroides vulgatus ATCC8482, Bacteroides ovatus, the Eubacterium hallii group, and Anaerostipes hadrus, either alone or in combination, could be used as noninvasive diagnostic biomarkers to discriminate children with T1DM from healthy controls. In addition, the functional changes in the T1DM-associated fecal microbiota also suggest that these fecal microbes were associated with altered functions and metabolic activities, such as glycan biosynthesis and metabolism and lipid metabolism, which might play vital roles in the pathogenesis and development of T1DM. CONCLUSION: Our present comprehensive investigation of the T1DM-associated fecal microbiota provides novel insights into the pathogenesis of the disease and sheds light on the diagnosis and treatment of T1DM. Baishideng Publishing Group Inc 2021-05-21 2021-05-21 /pmc/articles/PMC8130045/ /pubmed/34040330 http://dx.doi.org/10.3748/wjg.v27.i19.2394 Text en ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved. https://creativecommons.org/licenses/by-nc/4.0/This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
spellingShingle Observational Study
Liu, Xia
Cheng, Yi-Wen
Shao, Li
Sun, Shu-Hong
Wu, Jian
Song, Qing-Hai
Zou, Hong-Sheng
Ling, Zong-Xin
Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus: An observational study
title Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus: An observational study
title_full Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus: An observational study
title_fullStr Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus: An observational study
title_full_unstemmed Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus: An observational study
title_short Gut microbiota dysbiosis in Chinese children with type 1 diabetes mellitus: An observational study
title_sort gut microbiota dysbiosis in chinese children with type 1 diabetes mellitus: an observational study
topic Observational Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130045/
https://www.ncbi.nlm.nih.gov/pubmed/34040330
http://dx.doi.org/10.3748/wjg.v27.i19.2394
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