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Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis

As treatment of Staphylococcus aureus (S. aureus) osteomyelitis is often hindered by the development of antibiotic tolerance, novel antibacterial therapeutics are required. Here we found that the cell-free supernatant of Bacillus subtilis (B. subtilis CFS) killed planktonic and biofilm S. aureus, an...

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Autores principales: Zhang, Fan, Wang, Bowei, Liu, Shiluan, Chen, Yuhui, Lin, Yihuang, Liu, Zixian, Zhang, Xianrong, Yu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130150/
https://www.ncbi.nlm.nih.gov/pubmed/34001083
http://dx.doi.org/10.1186/s12934-021-01592-5
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author Zhang, Fan
Wang, Bowei
Liu, Shiluan
Chen, Yuhui
Lin, Yihuang
Liu, Zixian
Zhang, Xianrong
Yu, Bin
author_facet Zhang, Fan
Wang, Bowei
Liu, Shiluan
Chen, Yuhui
Lin, Yihuang
Liu, Zixian
Zhang, Xianrong
Yu, Bin
author_sort Zhang, Fan
collection PubMed
description As treatment of Staphylococcus aureus (S. aureus) osteomyelitis is often hindered by the development of antibiotic tolerance, novel antibacterial therapeutics are required. Here we found that the cell-free supernatant of Bacillus subtilis (B. subtilis CFS) killed planktonic and biofilm S. aureus, and increased S. aureus susceptibility to penicillin and gentamicin as well. Further study showed that B. subtilis CFS suppressed the expression of the genes involved in adhesive molecules (Cna and ClfA), virulence factor Hla, quorum sensing (argA, argB and RNAIII) and biofilm formation (Ica and sarA) in S. aureus. Additionally, our data showed that B. subtilis CFS changed the membrane components and increased membrane permeabilization of S. aureus. Finally, we demonstrated that B. subtilis CFS increased considerably the susceptibility of S. aureus to penicillin and effectively reduced S. aureus burdens in a mouse model of implant-associated osteomyelitis. These findings support that B. subtilis CFS may be a potential resistance-modifying agent for β-lactam antibiotics against S. aureus.
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spelling pubmed-81301502021-05-18 Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis Zhang, Fan Wang, Bowei Liu, Shiluan Chen, Yuhui Lin, Yihuang Liu, Zixian Zhang, Xianrong Yu, Bin Microb Cell Fact Research As treatment of Staphylococcus aureus (S. aureus) osteomyelitis is often hindered by the development of antibiotic tolerance, novel antibacterial therapeutics are required. Here we found that the cell-free supernatant of Bacillus subtilis (B. subtilis CFS) killed planktonic and biofilm S. aureus, and increased S. aureus susceptibility to penicillin and gentamicin as well. Further study showed that B. subtilis CFS suppressed the expression of the genes involved in adhesive molecules (Cna and ClfA), virulence factor Hla, quorum sensing (argA, argB and RNAIII) and biofilm formation (Ica and sarA) in S. aureus. Additionally, our data showed that B. subtilis CFS changed the membrane components and increased membrane permeabilization of S. aureus. Finally, we demonstrated that B. subtilis CFS increased considerably the susceptibility of S. aureus to penicillin and effectively reduced S. aureus burdens in a mouse model of implant-associated osteomyelitis. These findings support that B. subtilis CFS may be a potential resistance-modifying agent for β-lactam antibiotics against S. aureus. BioMed Central 2021-05-17 /pmc/articles/PMC8130150/ /pubmed/34001083 http://dx.doi.org/10.1186/s12934-021-01592-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhang, Fan
Wang, Bowei
Liu, Shiluan
Chen, Yuhui
Lin, Yihuang
Liu, Zixian
Zhang, Xianrong
Yu, Bin
Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis
title Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis
title_full Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis
title_fullStr Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis
title_full_unstemmed Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis
title_short Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis
title_sort bacillus subtilis revives conventional antibiotics against staphylococcus aureus osteomyelitis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130150/
https://www.ncbi.nlm.nih.gov/pubmed/34001083
http://dx.doi.org/10.1186/s12934-021-01592-5
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