Cargando…
ddRAD sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae)
BACKGROUND: Brown marmorated stink bug (BMSB), Halyomorpha halys (Hemiptera: Pentatomidae) is native to East Asia but has invaded many countries in the world. BMSB is a polyphagous insect pest and causes significant economic losses to agriculture worldwide. Knowledge on the genetic diversity among B...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130256/ https://www.ncbi.nlm.nih.gov/pubmed/34000993 http://dx.doi.org/10.1186/s12864-021-07678-z |
_version_ | 1783694478114029568 |
---|---|
author | Yan, Juncong Vétek, Gábor Pal, Chandan Zhang, Jinping Gmati, Rania Fan, Qing-Hai Gunawardana, Disna N. Burne, Allan Anderson, Diane Balan, Rebijith Kayattukandy George, Sherly Farkas, Péter Li, Dongmei |
author_facet | Yan, Juncong Vétek, Gábor Pal, Chandan Zhang, Jinping Gmati, Rania Fan, Qing-Hai Gunawardana, Disna N. Burne, Allan Anderson, Diane Balan, Rebijith Kayattukandy George, Sherly Farkas, Péter Li, Dongmei |
author_sort | Yan, Juncong |
collection | PubMed |
description | BACKGROUND: Brown marmorated stink bug (BMSB), Halyomorpha halys (Hemiptera: Pentatomidae) is native to East Asia but has invaded many countries in the world. BMSB is a polyphagous insect pest and causes significant economic losses to agriculture worldwide. Knowledge on the genetic diversity among BMSB populations is scarce but is essential to understand the patterns of colonization and invasion history of local populations. Efforts have been made to assess the genetic diversity of BMSB using partial mitochondrial DNA sequences but genetic divergence on mitochondria is not high enough to precisely accurately identify and distinguish various BMSB populations. Therefore, in this study, we applied a ddRAD (double digest restriction-site associated DNA) sequencing approach to ascertain the genetic diversity of BMSB populations collected from 12 countries (2 native and 10 invaded) across four continents with the ultimate aim to trace the origin of BMSBs intercepted during border inspections and post-border surveillance. RESULT: A total of 1775 high confidence single nucleotide polymorphisms (SNPs) were identified from ddRAD sequencing data collected from 389 adult BMSB individuals. Principal component analysis (PCA) of the identified SNPs indicated the existence of two main distinct genetic clusters representing individuals sampled from regions where BMSB is native to, China and Japan, respectively, and one broad cluster comprised individuals sampled from countries which have been invaded by BMSB. The population genetic structure analysis further discriminated the genetic diversity among the BMSB populations at a higher resolution and distinguished them into five potential genetic clusters. CONCLUSION: The study revealed hidden genetic diversity among the studied BMSB populations across the continents. The BMSB populations from Japan were genetically distant from the other studied populations. Similarly, the BMSB populations from China were also genetically differentiated from the Japanese and other populations. Further genetic structure analysis revealed the presence of at least three genetic clusters of BMSB in the invaded countries, possibly originating via multiple invasions. Furthermore, this study has produced novel set of SNP markers to enhance the knowledge of genetic diversity among BMSB populations and demonstrates the potential to trace the origin of BMSB individuals for future invasion events. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-07678-z. |
format | Online Article Text |
id | pubmed-8130256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-81302562021-05-18 ddRAD sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) Yan, Juncong Vétek, Gábor Pal, Chandan Zhang, Jinping Gmati, Rania Fan, Qing-Hai Gunawardana, Disna N. Burne, Allan Anderson, Diane Balan, Rebijith Kayattukandy George, Sherly Farkas, Péter Li, Dongmei BMC Genomics Research Article BACKGROUND: Brown marmorated stink bug (BMSB), Halyomorpha halys (Hemiptera: Pentatomidae) is native to East Asia but has invaded many countries in the world. BMSB is a polyphagous insect pest and causes significant economic losses to agriculture worldwide. Knowledge on the genetic diversity among BMSB populations is scarce but is essential to understand the patterns of colonization and invasion history of local populations. Efforts have been made to assess the genetic diversity of BMSB using partial mitochondrial DNA sequences but genetic divergence on mitochondria is not high enough to precisely accurately identify and distinguish various BMSB populations. Therefore, in this study, we applied a ddRAD (double digest restriction-site associated DNA) sequencing approach to ascertain the genetic diversity of BMSB populations collected from 12 countries (2 native and 10 invaded) across four continents with the ultimate aim to trace the origin of BMSBs intercepted during border inspections and post-border surveillance. RESULT: A total of 1775 high confidence single nucleotide polymorphisms (SNPs) were identified from ddRAD sequencing data collected from 389 adult BMSB individuals. Principal component analysis (PCA) of the identified SNPs indicated the existence of two main distinct genetic clusters representing individuals sampled from regions where BMSB is native to, China and Japan, respectively, and one broad cluster comprised individuals sampled from countries which have been invaded by BMSB. The population genetic structure analysis further discriminated the genetic diversity among the BMSB populations at a higher resolution and distinguished them into five potential genetic clusters. CONCLUSION: The study revealed hidden genetic diversity among the studied BMSB populations across the continents. The BMSB populations from Japan were genetically distant from the other studied populations. Similarly, the BMSB populations from China were also genetically differentiated from the Japanese and other populations. Further genetic structure analysis revealed the presence of at least three genetic clusters of BMSB in the invaded countries, possibly originating via multiple invasions. Furthermore, this study has produced novel set of SNP markers to enhance the knowledge of genetic diversity among BMSB populations and demonstrates the potential to trace the origin of BMSB individuals for future invasion events. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-021-07678-z. BioMed Central 2021-05-17 /pmc/articles/PMC8130256/ /pubmed/34000993 http://dx.doi.org/10.1186/s12864-021-07678-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Yan, Juncong Vétek, Gábor Pal, Chandan Zhang, Jinping Gmati, Rania Fan, Qing-Hai Gunawardana, Disna N. Burne, Allan Anderson, Diane Balan, Rebijith Kayattukandy George, Sherly Farkas, Péter Li, Dongmei ddRAD sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) |
title | ddRAD sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) |
title_full | ddRAD sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) |
title_fullStr | ddRAD sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) |
title_full_unstemmed | ddRAD sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) |
title_short | ddRAD sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae) |
title_sort | ddrad sequencing: an emerging technology added to the biosecurity toolbox for tracing the origin of brown marmorated stink bug, halyomorpha halys (hemiptera: pentatomidae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130256/ https://www.ncbi.nlm.nih.gov/pubmed/34000993 http://dx.doi.org/10.1186/s12864-021-07678-z |
work_keys_str_mv | AT yanjuncong ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT vetekgabor ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT palchandan ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT zhangjinping ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT gmatirania ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT fanqinghai ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT gunawardanadisnan ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT burneallan ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT andersondiane ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT balanrebijithkayattukandy ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT georgesherly ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT farkaspeter ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae AT lidongmei ddradsequencinganemergingtechnologyaddedtothebiosecuritytoolboxfortracingtheoriginofbrownmarmoratedstinkbughalyomorphahalyshemipterapentatomidae |