Cargando…

Subtype selective fluorescent ligands based on ICI 118,551 to study the human β2‐adrenoceptor in CRISPR/Cas9 genome‐edited HEK293T cells at low expression levels

Fluorescent ligand technologies have proved to be powerful tools to improve our understanding of ligand‐receptor interactions. Here we have characterized a small focused library of nine fluorescent ligands based on the highly selective β(2)‐adrenoceptor (β(2)AR) antagonist ICI 118,551. The majority...

Descripción completa

Detalles Bibliográficos
Autores principales:	Goulding, Joëlle, Mistry, Sarah J., Soave, Mark, Woolard, Jeanette, Briddon, Stephen J., White, Carl W., Kellam, Barrie, Hill, Stephen J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130569/ https://www.ncbi.nlm.nih.gov/pubmed/34003582 http://dx.doi.org/10.1002/prp2.779

Ejemplares similares

β(1) Adrenoceptor antagonistic effects of the supposedly selective β(2) adrenoceptor antagonist ICI 118,551 on the positive inotropic effect of adrenaline in murine hearts
por: Pecha, Simon, et al.
Publicado: (2015)

The β2-adrenergic receptor antagonist ICI-118,551 blocks the constitutively activated HIF signalling in hemangioblastomas from von Hippel-Lindau disease
por: Cuesta, A. M., et al.
Publicado: (2019)

Effects of norepinephrine and β2 receptor antagonist ICI 118,551 on whisker hair follicle mechanoreceptors dissatisfy Merkel discs being adrenergic synapses
por: Sonekatsu, Mayumi, et al.
Publicado: (2019)

Short-term effects of β2-AR blocker ICI 118,551 on sarcoplasmic reticulum SERCA2a and cardiac function of rats with heart failure
por: Gong, Haibin, et al.
Publicado: (2016)

Use of a new proximity assay (NanoBRET) to investigate the ligand‐binding characteristics of three fluorescent ligands to the human β (1)‐adrenoceptor expressed in HEK‐293 cells
por: Soave, Mark, et al.
Publicado: (2016)

NanoBiT Complementation to Monitor Agonist-Induced Adenosine A(1) Receptor Internalization
por: Soave, Mark, et al.
Publicado: (2019)

Monitoring Allosteric Interactions with CXCR4 Using NanoBiT Conjugated Nanobodies
por: Soave, Mark, et al.
Publicado: (2020)

Novel selective β(1)-adrenoceptor antagonists for concomitant cardiovascular and respiratory disease
por: Baker, Jillian G., et al.
Publicado: (2017)

Effects of receptor tyrosine kinase inhibitors on VEGF(165)a- and VEGF(165)b-stimulated gene transcription in HEK-293 cells expressing human VEGFR2
por: Carter, Joanne J, et al.
Publicado: (2015)

A monoclonal antibody raised against a thermo-stabilised β(1)-adrenoceptor interacts with extracellular loop 2 and acts as a negative allosteric modulator of a sub-set of β(1)-adrenoceptors expressed in stable cell lines
por: Soave, Mark, et al.
Publicado: (2018)

Characterisation of endogenous A(2A) and A(2B) receptor-mediated cyclic AMP responses in HEK 293 cells using the GloSensor™ biosensor: Evidence for an allosteric mechanism of action for the A(2B)-selective antagonist PSB 603
por: Goulding, Joelle, et al.
Publicado: (2018)

Synthesis and in Vitro and in Vivo Characterization of Highly β(1)-Selective β-Adrenoceptor Partial Agonists
por: Mistry, Shailesh N., et al.
Publicado: (2013)

Echinacea-induced cytosolic Ca(2+ )elevation in HEK293
por: Wu, Lankun, et al.
Publicado: (2010)

Small-Molecule Fluorescent Ligands for the CXCR4 Chemokine Receptor
por: Dekkers, Sebastian, et al.
Publicado: (2023)

Effect of a toggle switch mutation in TM6 of the human adenosine A(3) receptor on Gi protein-dependent signalling and Gi-independent receptor internalization
por: Stoddart, Leigh A, et al.
Publicado: (2014)

Synthesis and Characterization of High-Affinity 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene-Labeled Fluorescent Ligands for Human β-Adrenoceptors
por: Baker, Jillian G., et al.
Publicado: (2011)

Negative cooperativity across β(1)-adrenoceptor homodimers provides insights into the nature of the secondary low-affinity CGP 12177 β1-adrenoceptor binding conformation
por: Gherbi, Karolina, et al.
Publicado: (2015)

HEK293S Cells Have Functional Retinoid Processing Machinery
por: Brueggemann, Lioubov I., et al.
Publicado: (2002)

Effects of endotoxin on pacemaker funny current in HEK 293 cells
por: Papaioannou, VP, et al.
Publicado: (2011)

Effect of PTTG on endogenous gene expression in HEK 293 cells
por: Panguluri, Siva K, et al.
Publicado: (2009)

Analysis of DNA topology of EBV minichromosomes in HEK 293 cells
por: Castán, Alicia, et al.
Publicado: (2017)

HEK293T cell lines defective for O-linked glycosylation
por: Termini, James M., et al.
Publicado: (2017)

Microtubule Integrity Is Associated with the Functional Activity of Mitochondria in HEK293
por: Cho, Min Jeong, et al.
Publicado: (2021)

Detection of genome‐edited and endogenously expressed G protein‐coupled receptors
por: Soave, Mark, et al.
Publicado: (2021)

Development of a Model System for Tick-Borne Flavivirus Persistence in HEK 293T Cells
por: Mlera, Luwanika, et al.
Publicado: (2015)

Ligand-directed covalent labelling of a GPCR with a fluorescent tag in live cells
por: Stoddart, Leigh A., et al.
Publicado: (2020)

Kinetic analysis of antagonist-occupied adenosine-A(3) receptors within membrane microdomains of individual cells provides evidence of receptor dimerization and allosterism
por: Corriden, Ross, et al.
Publicado: (2014)

Involvement of β‐adrenoceptors in the cardiovascular responses induced by selective adenosine A(2A) and A(2B) receptor agonists
por: Wragg, Edward S., et al.
Publicado: (2022)

CD133 induces tumour-initiating properties in HEK293 cells
por: Canis, Martin, et al.
Publicado: (2012)

Differences in the production of hyperglycosylated IFN alpha in CHO and HEK 293 cells
por: Gugliotta, Agustina, et al.
Publicado: (2013)

Cytotoxicity and genotoxicity caused by yttrium oxide nanoparticles in HEK293 cells
por: Selvaraj, Vellaisamy, et al.
Publicado: (2014)

Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature
por: Lin, Chi-Yen, et al.
Publicado: (2015)

Cellular signaling pathways modulated by influenza infection in HEK293 cells
por: Manceur, Aziza P, et al.
Publicado: (2015)

HEK293T Cells Are Heterozygous for CCR5 Delta 32 Mutation
por: Qi, Chunxia, et al.
Publicado: (2016)

Distinct mechanical behavior of HEK293 cells in adherent and suspended states
por: Haghparast, Seyed Mohammad Ali, et al.
Publicado: (2015)

Using HEK293T Expression System to Study Photoactive Plant Cryptochromes
por: Yang, Liang, et al.
Publicado: (2016)

Lack of current observed in HEK293 cells expressing NALCN channels
por: Egan, Jennifer M., et al.
Publicado: (2018)

Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells
por: Blessing, Daniel, et al.
Publicado: (2018)

Degradation of Extracellular NAD(+) Intermediates in Cultures of Human HEK293 Cells
por: Kulikova, Veronika, et al.
Publicado: (2019)

Optimization of Experimental Variables Influencing Apoptosome Biosensor in HEK293T Cells
por: Oladzad, Azarakhsh, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_dn2af9joed0lqlgni4oglu3tdd