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Phytotoxic Metabolites Produce by Fusarium oxysporum f. sp. cubense Race 2

Banana is a major tropical fruit crop but banana production worldwide is seriously threatened due to Fusarium wilt. Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt of banana (also referred as Panama disease) is an asexual, soil inhabiting facultative parasite. Foc isolates...

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Autores principales: Portal González, N., Soler, A., Ribadeneira, C., Solano, J., Portieles, Roxana, Herrera Isla, L., Companioni, B., Borras-Hidalgo, Orlando, Santos Bermudez, Ramon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130618/
https://www.ncbi.nlm.nih.gov/pubmed/34017315
http://dx.doi.org/10.3389/fmicb.2021.629395
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author Portal González, N.
Soler, A.
Ribadeneira, C.
Solano, J.
Portieles, Roxana
Herrera Isla, L.
Companioni, B.
Borras-Hidalgo, Orlando
Santos Bermudez, Ramon
author_facet Portal González, N.
Soler, A.
Ribadeneira, C.
Solano, J.
Portieles, Roxana
Herrera Isla, L.
Companioni, B.
Borras-Hidalgo, Orlando
Santos Bermudez, Ramon
author_sort Portal González, N.
collection PubMed
description Banana is a major tropical fruit crop but banana production worldwide is seriously threatened due to Fusarium wilt. Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt of banana (also referred as Panama disease) is an asexual, soil inhabiting facultative parasite. Foc isolates can be classified into three races that are not defined genetically, but for their pathogenicity to different banana cultivars. Despite mycotoxins being some of the best studied virulence factors of phytopathogenic fungi and these have been useful for the prediction of Foc virulence on banana plants, toxins produced by Foc race 2 strains have not been previously identified. The aim of this contribution was to identify the phytotoxic metabolites closely related to banana wilt caused by a Foc race 2 strain. We used an in vitro bioassay on detached banana leaves to evaluate the specificity of the microbial culture filtrates before a partial purification and further identification of Foc race 2 phytotoxins. A 29-day-old host-specific culture filtrate was obtained but specificity of culture filtrate was unrecovered after partial purification. The non-specific phytotoxins were characterized as fusaric acid, beauvericin, and enniatin A. Whereas some, if not all, of these phytotoxins are important virulence factors, a proteinaceous fraction from the specific 29-day-old culture filtrate protected the leaves of the resistant banana cultivar from damage caused by such phytotoxic metabolites.
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spelling pubmed-81306182021-05-19 Phytotoxic Metabolites Produce by Fusarium oxysporum f. sp. cubense Race 2 Portal González, N. Soler, A. Ribadeneira, C. Solano, J. Portieles, Roxana Herrera Isla, L. Companioni, B. Borras-Hidalgo, Orlando Santos Bermudez, Ramon Front Microbiol Microbiology Banana is a major tropical fruit crop but banana production worldwide is seriously threatened due to Fusarium wilt. Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt of banana (also referred as Panama disease) is an asexual, soil inhabiting facultative parasite. Foc isolates can be classified into three races that are not defined genetically, but for their pathogenicity to different banana cultivars. Despite mycotoxins being some of the best studied virulence factors of phytopathogenic fungi and these have been useful for the prediction of Foc virulence on banana plants, toxins produced by Foc race 2 strains have not been previously identified. The aim of this contribution was to identify the phytotoxic metabolites closely related to banana wilt caused by a Foc race 2 strain. We used an in vitro bioassay on detached banana leaves to evaluate the specificity of the microbial culture filtrates before a partial purification and further identification of Foc race 2 phytotoxins. A 29-day-old host-specific culture filtrate was obtained but specificity of culture filtrate was unrecovered after partial purification. The non-specific phytotoxins were characterized as fusaric acid, beauvericin, and enniatin A. Whereas some, if not all, of these phytotoxins are important virulence factors, a proteinaceous fraction from the specific 29-day-old culture filtrate protected the leaves of the resistant banana cultivar from damage caused by such phytotoxic metabolites. Frontiers Media S.A. 2021-04-15 /pmc/articles/PMC8130618/ /pubmed/34017315 http://dx.doi.org/10.3389/fmicb.2021.629395 Text en Copyright © 2021 Portal González, Soler, Ribadeneira, Solano, Portieles, Herrera Isla, Companioni, Borras-Hidalgo and Santos Bermudez. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Portal González, N.
Soler, A.
Ribadeneira, C.
Solano, J.
Portieles, Roxana
Herrera Isla, L.
Companioni, B.
Borras-Hidalgo, Orlando
Santos Bermudez, Ramon
Phytotoxic Metabolites Produce by Fusarium oxysporum f. sp. cubense Race 2
title Phytotoxic Metabolites Produce by Fusarium oxysporum f. sp. cubense Race 2
title_full Phytotoxic Metabolites Produce by Fusarium oxysporum f. sp. cubense Race 2
title_fullStr Phytotoxic Metabolites Produce by Fusarium oxysporum f. sp. cubense Race 2
title_full_unstemmed Phytotoxic Metabolites Produce by Fusarium oxysporum f. sp. cubense Race 2
title_short Phytotoxic Metabolites Produce by Fusarium oxysporum f. sp. cubense Race 2
title_sort phytotoxic metabolites produce by fusarium oxysporum f. sp. cubense race 2
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8130618/
https://www.ncbi.nlm.nih.gov/pubmed/34017315
http://dx.doi.org/10.3389/fmicb.2021.629395
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