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Protective Effect of Lemon Peel Extract on Oxidative Stress in H9c2 Rat Heart Cell Injury

AIM: Lemon peel, a traditional Chinese medicine, was tested in this study for its novel application in inhibiting cellular oxidative stress, and the effect of lemon peel extract (LPE) on protecting H9c2 rat heart cells from oxidative stress was investigated. METHODS: The scavenging effects of LPE on...

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Autores principales: Wang, Jun, Zhai, Yulin, Ou, Mingguang, Bian, Yunfeng, Tang, Chenglong, Zhang, Wanchao, Cheng, Yujiao, Li, Guijie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131012/
https://www.ncbi.nlm.nih.gov/pubmed/34017169
http://dx.doi.org/10.2147/DDDT.S304624
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author Wang, Jun
Zhai, Yulin
Ou, Mingguang
Bian, Yunfeng
Tang, Chenglong
Zhang, Wanchao
Cheng, Yujiao
Li, Guijie
author_facet Wang, Jun
Zhai, Yulin
Ou, Mingguang
Bian, Yunfeng
Tang, Chenglong
Zhang, Wanchao
Cheng, Yujiao
Li, Guijie
author_sort Wang, Jun
collection PubMed
description AIM: Lemon peel, a traditional Chinese medicine, was tested in this study for its novel application in inhibiting cellular oxidative stress, and the effect of lemon peel extract (LPE) on protecting H9c2 rat heart cells from oxidative stress was investigated. METHODS: The scavenging effects of LPE on 1,1-diphenyl-2-picryhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radicals were measured in extracellular experiments. The 3-(4,5-dimethyl-2-thiazolinyl)-2,5-diphenyl-2-h-tetrazolylammonium bromide (MTT) assay was used to detect the cell survival rate. The cell supernatant and intracellular oxidation-related indicators were detected by a kit, and the mRNA expression in H9c2 cells was detected by quantitative polymerase chain reaction (qPCR). The chemical substances of LPE were analyzed by high-performance liquid chromatography (HPLC). RESULTS: The results showed that LPE exhibited better DPPH and ABTS free radical scavenging abilities than vitamin C. Compared with the cells in the normal state (control group), the cell survival rate in the model group decreased, and the level of lactate dehydrogenase (LDH) increased, the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) decreased, and the content of malondialdehyde (MDA) increased. Compared with the control group, the expression of Bcl-2-related X protein (Bax), caspase-3, nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) in the model group was increased, and the expression of B-cell lymphoma-2 (Bcl-2) was reduced. Compared with the model group, LPE treatment improved the cell survival rate, reduced the levels of LDH and MDA, increased the levels of SOD, CAT, and GSH, downregulated the expression of Bax, caspase-3, Nrf2 and HO-1, and upregulated the expression of Bcl-2. The composition analysis showed that LPE contained catechin, rutin, naringin, quercetin, and hesperidin. CONCLUSION: The results indicated that LPE could protect H9c2 cells from oxidative stress through five active components. LPE has the potential to be developed into natural medicine or health food for the inhibition of cell oxidative damage.
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spelling pubmed-81310122021-05-19 Protective Effect of Lemon Peel Extract on Oxidative Stress in H9c2 Rat Heart Cell Injury Wang, Jun Zhai, Yulin Ou, Mingguang Bian, Yunfeng Tang, Chenglong Zhang, Wanchao Cheng, Yujiao Li, Guijie Drug Des Devel Ther Original Research AIM: Lemon peel, a traditional Chinese medicine, was tested in this study for its novel application in inhibiting cellular oxidative stress, and the effect of lemon peel extract (LPE) on protecting H9c2 rat heart cells from oxidative stress was investigated. METHODS: The scavenging effects of LPE on 1,1-diphenyl-2-picryhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radicals were measured in extracellular experiments. The 3-(4,5-dimethyl-2-thiazolinyl)-2,5-diphenyl-2-h-tetrazolylammonium bromide (MTT) assay was used to detect the cell survival rate. The cell supernatant and intracellular oxidation-related indicators were detected by a kit, and the mRNA expression in H9c2 cells was detected by quantitative polymerase chain reaction (qPCR). The chemical substances of LPE were analyzed by high-performance liquid chromatography (HPLC). RESULTS: The results showed that LPE exhibited better DPPH and ABTS free radical scavenging abilities than vitamin C. Compared with the cells in the normal state (control group), the cell survival rate in the model group decreased, and the level of lactate dehydrogenase (LDH) increased, the levels of superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) decreased, and the content of malondialdehyde (MDA) increased. Compared with the control group, the expression of Bcl-2-related X protein (Bax), caspase-3, nuclear factor erythroid 2-related factor 2 (Nrf2), and heme oxygenase-1 (HO-1) in the model group was increased, and the expression of B-cell lymphoma-2 (Bcl-2) was reduced. Compared with the model group, LPE treatment improved the cell survival rate, reduced the levels of LDH and MDA, increased the levels of SOD, CAT, and GSH, downregulated the expression of Bax, caspase-3, Nrf2 and HO-1, and upregulated the expression of Bcl-2. The composition analysis showed that LPE contained catechin, rutin, naringin, quercetin, and hesperidin. CONCLUSION: The results indicated that LPE could protect H9c2 cells from oxidative stress through five active components. LPE has the potential to be developed into natural medicine or health food for the inhibition of cell oxidative damage. Dove 2021-05-14 /pmc/articles/PMC8131012/ /pubmed/34017169 http://dx.doi.org/10.2147/DDDT.S304624 Text en © 2021 Wang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wang, Jun
Zhai, Yulin
Ou, Mingguang
Bian, Yunfeng
Tang, Chenglong
Zhang, Wanchao
Cheng, Yujiao
Li, Guijie
Protective Effect of Lemon Peel Extract on Oxidative Stress in H9c2 Rat Heart Cell Injury
title Protective Effect of Lemon Peel Extract on Oxidative Stress in H9c2 Rat Heart Cell Injury
title_full Protective Effect of Lemon Peel Extract on Oxidative Stress in H9c2 Rat Heart Cell Injury
title_fullStr Protective Effect of Lemon Peel Extract on Oxidative Stress in H9c2 Rat Heart Cell Injury
title_full_unstemmed Protective Effect of Lemon Peel Extract on Oxidative Stress in H9c2 Rat Heart Cell Injury
title_short Protective Effect of Lemon Peel Extract on Oxidative Stress in H9c2 Rat Heart Cell Injury
title_sort protective effect of lemon peel extract on oxidative stress in h9c2 rat heart cell injury
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131012/
https://www.ncbi.nlm.nih.gov/pubmed/34017169
http://dx.doi.org/10.2147/DDDT.S304624
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