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The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina

Low-dose atropine helps to control myopia progression with few side effects. However, the impact of atropine, a non-selective muscarinic Acetylcholine (ACh) receptor antagonist, on retinal ganglion cells (RGCs) remains unclear. After immersing the cornea and adjacent conjunctiva of enucleated eyes i...

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Autores principales: Wang, Qin, Banerjee, Seema, So, ChungHim, Qiu, ChunTing, Sze, YingHon, Lam, Thomas Chuen, To, Chi-Ho, Pan, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131517/
https://www.ncbi.nlm.nih.gov/pubmed/34025362
http://dx.doi.org/10.3389/fncel.2021.664491
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author Wang, Qin
Banerjee, Seema
So, ChungHim
Qiu, ChunTing
Sze, YingHon
Lam, Thomas Chuen
To, Chi-Ho
Pan, Feng
author_facet Wang, Qin
Banerjee, Seema
So, ChungHim
Qiu, ChunTing
Sze, YingHon
Lam, Thomas Chuen
To, Chi-Ho
Pan, Feng
author_sort Wang, Qin
collection PubMed
description Low-dose atropine helps to control myopia progression with few side effects. However, the impact of atropine, a non-selective muscarinic Acetylcholine (ACh) receptor antagonist, on retinal ganglion cells (RGCs) remains unclear. After immersing the cornea and adjacent conjunctiva of enucleated eyes in 0.05% (approximately 800 μM) atropine solution for 30 min, the atropine concentration reached in the retina was below 2 μM. After direct superfusion of the retina with 1 μM atropine (considering that the clinical application of 0.05% atropine eye drops will be diluted over time due to tear flow for 30 min), no noticeable changes in the morphology of ON and OFF alpha RGCs (αRGCs) were observed. Atropine affected the light-evoked responses of ON and OFF αRGCs in a dose- and time-dependent fashion. Direct application of less than 100 μM atropine on the retina did not affect light-evoked responses. The time latency of light-induced responses of ON or OFF αRGCs did not change after the application of 0.05–100 μM atropine for 5 min. However, 50 μM atropine extended the threshold of joint inter-spike interval (ISI) distribution of the RGCs. These results indicated that low-dose atropine (<0.5 μM; equal to 1% atropine topical application) did not interfere with spike frequency, the pattern of synchronized firing between OFF αRGCs, or the threshold of joint ISI distribution of αRGCs. The application of atropine unmasked inhibition to induce ON responses from certain OFF RGCs, possibly via the GABAergic pathway, potentially affecting visual information processing.
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spelling pubmed-81315172021-05-20 The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina Wang, Qin Banerjee, Seema So, ChungHim Qiu, ChunTing Sze, YingHon Lam, Thomas Chuen To, Chi-Ho Pan, Feng Front Cell Neurosci Cellular Neuroscience Low-dose atropine helps to control myopia progression with few side effects. However, the impact of atropine, a non-selective muscarinic Acetylcholine (ACh) receptor antagonist, on retinal ganglion cells (RGCs) remains unclear. After immersing the cornea and adjacent conjunctiva of enucleated eyes in 0.05% (approximately 800 μM) atropine solution for 30 min, the atropine concentration reached in the retina was below 2 μM. After direct superfusion of the retina with 1 μM atropine (considering that the clinical application of 0.05% atropine eye drops will be diluted over time due to tear flow for 30 min), no noticeable changes in the morphology of ON and OFF alpha RGCs (αRGCs) were observed. Atropine affected the light-evoked responses of ON and OFF αRGCs in a dose- and time-dependent fashion. Direct application of less than 100 μM atropine on the retina did not affect light-evoked responses. The time latency of light-induced responses of ON or OFF αRGCs did not change after the application of 0.05–100 μM atropine for 5 min. However, 50 μM atropine extended the threshold of joint inter-spike interval (ISI) distribution of the RGCs. These results indicated that low-dose atropine (<0.5 μM; equal to 1% atropine topical application) did not interfere with spike frequency, the pattern of synchronized firing between OFF αRGCs, or the threshold of joint ISI distribution of αRGCs. The application of atropine unmasked inhibition to induce ON responses from certain OFF RGCs, possibly via the GABAergic pathway, potentially affecting visual information processing. Frontiers Media S.A. 2021-05-05 /pmc/articles/PMC8131517/ /pubmed/34025362 http://dx.doi.org/10.3389/fncel.2021.664491 Text en Copyright © 2021 Wang, Banerjee, So, Qiu, Sze, Lam, To and Pan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular Neuroscience
Wang, Qin
Banerjee, Seema
So, ChungHim
Qiu, ChunTing
Sze, YingHon
Lam, Thomas Chuen
To, Chi-Ho
Pan, Feng
The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina
title The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina
title_full The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina
title_fullStr The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina
title_full_unstemmed The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina
title_short The Effect of Low-Dose Atropine on Alpha Ganglion Cell Signaling in the Mouse Retina
title_sort effect of low-dose atropine on alpha ganglion cell signaling in the mouse retina
topic Cellular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131517/
https://www.ncbi.nlm.nih.gov/pubmed/34025362
http://dx.doi.org/10.3389/fncel.2021.664491
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