A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis

The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPO...

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Autores principales: Xun, Guanhua, Lane, Stephan Thomas, Petrov, Vassily Andrew, Pepa, Brandon Elliott, Zhao, Huimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131735/
https://www.ncbi.nlm.nih.gov/pubmed/34006857
http://dx.doi.org/10.1038/s41467-021-23185-x
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author Xun, Guanhua
Lane, Stephan Thomas
Petrov, Vassily Andrew
Pepa, Brandon Elliott
Zhao, Huimin
author_facet Xun, Guanhua
Lane, Stephan Thomas
Petrov, Vassily Andrew
Pepa, Brandon Elliott
Zhao, Huimin
author_sort Xun, Guanhua
collection PubMed
description The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities.
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spelling pubmed-81317352021-05-24 A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis Xun, Guanhua Lane, Stephan Thomas Petrov, Vassily Andrew Pepa, Brandon Elliott Zhao, Huimin Nat Commun Article The need for rapid, accurate, and scalable testing systems for COVID-19 diagnosis is clear and urgent. Here, we report a rapid Scalable and Portable Testing (SPOT) system consisting of a rapid, highly sensitive, and accurate assay and a battery-powered portable device for COVID-19 diagnosis. The SPOT assay comprises a one-pot reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) followed by PfAgo-based target sequence detection. It is capable of detecting the N gene and E gene in a multiplexed reaction with the limit of detection (LoD) of 0.44 copies/μL and 1.09 copies/μL, respectively, in SARS-CoV-2 virus-spiked saliva samples within 30 min. Moreover, the SPOT system is used to analyze 104 clinical saliva samples and identified 28/30 (93.3% sensitivity) SARS-CoV-2 positive samples (100% sensitivity if LoD is considered) and 73/74 (98.6% specificity) SARS-CoV-2 negative samples. This combination of speed, accuracy, sensitivity, and portability will enable high-volume, low-cost access to areas in need of urgent COVID-19 testing capabilities. Nature Publishing Group UK 2021-05-18 /pmc/articles/PMC8131735/ /pubmed/34006857 http://dx.doi.org/10.1038/s41467-021-23185-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Xun, Guanhua
Lane, Stephan Thomas
Petrov, Vassily Andrew
Pepa, Brandon Elliott
Zhao, Huimin
A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
title A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
title_full A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
title_fullStr A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
title_full_unstemmed A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
title_short A rapid, accurate, scalable, and portable testing system for COVID-19 diagnosis
title_sort rapid, accurate, scalable, and portable testing system for covid-19 diagnosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131735/
https://www.ncbi.nlm.nih.gov/pubmed/34006857
http://dx.doi.org/10.1038/s41467-021-23185-x
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