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Tomato SlCDF3 Delays Flowering Time by Regulating Different FT-Like Genes Under Long-Day and Short-Day Conditions

Photoperiod is a crucial inducer of plant flowering. Cycling DOF factors (CDFs) play pivotal roles in the flowering of long-day (LD) and short-day (SD) plants. However, the functions of CDFs in the photoperiod regulated flowering remain unclear in day-neutral plants. In the present study, tomato (So...

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Autores principales: Xu, Dawei, Li, Xueou, Wu, Xue, Meng, Lili, Zou, Zhirong, Bao, Encai, Bian, Zhonghua, Cao, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131850/
https://www.ncbi.nlm.nih.gov/pubmed/34025696
http://dx.doi.org/10.3389/fpls.2021.650068
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author Xu, Dawei
Li, Xueou
Wu, Xue
Meng, Lili
Zou, Zhirong
Bao, Encai
Bian, Zhonghua
Cao, Kai
author_facet Xu, Dawei
Li, Xueou
Wu, Xue
Meng, Lili
Zou, Zhirong
Bao, Encai
Bian, Zhonghua
Cao, Kai
author_sort Xu, Dawei
collection PubMed
description Photoperiod is a crucial inducer of plant flowering. Cycling DOF factors (CDFs) play pivotal roles in the flowering of long-day (LD) and short-day (SD) plants. However, the functions of CDFs in the photoperiod regulated flowering remain unclear in day-neutral plants. In the present study, tomato (Solanum lycopersicum L. cv. “Ailsa Craig”) seedlings of the wild-type and transgenic lines of overexpressing CDFs were treated with different photoperiods. The flowering time and the expression pattern of SlCDFs and other FT-like genes were investigated. The results showed that tomato SlCDF1, SlCDF2, SlCDF3, SlCDF4, and SlCDF5 are homologs to Arabidopsis cycling DOF factor 1 (AtCDF1). SlCDF1–5 expression levels were influenced by the developmental stage and the tissue location, and notably, the expression patterns throughout light environments showed two opposite trends. Among the SlCDF1–5 overexpression transgenic lines, overexpressing SlCDF3 delayed flowering time in both LD (16 h light/8 h dark) and SD (8 h light/16 h dark) conditions. Furthermore, SlCDF3 led to an increase in the mRNA level of SlSP5G, a tomato FT-like gene, in LD conditions, while the transcription level of the other two FT-like genes, SlSP5G2 and SlSP5G3, were up-regulated in SD conditions. Taken together, at the transcription level, our results demonstrated that SlCDF3 played a significant role in controlling tomato flowering under LD and SD conditions, possibly through directly or indirectly regulating FT-like genes.
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spelling pubmed-81318502021-05-20 Tomato SlCDF3 Delays Flowering Time by Regulating Different FT-Like Genes Under Long-Day and Short-Day Conditions Xu, Dawei Li, Xueou Wu, Xue Meng, Lili Zou, Zhirong Bao, Encai Bian, Zhonghua Cao, Kai Front Plant Sci Plant Science Photoperiod is a crucial inducer of plant flowering. Cycling DOF factors (CDFs) play pivotal roles in the flowering of long-day (LD) and short-day (SD) plants. However, the functions of CDFs in the photoperiod regulated flowering remain unclear in day-neutral plants. In the present study, tomato (Solanum lycopersicum L. cv. “Ailsa Craig”) seedlings of the wild-type and transgenic lines of overexpressing CDFs were treated with different photoperiods. The flowering time and the expression pattern of SlCDFs and other FT-like genes were investigated. The results showed that tomato SlCDF1, SlCDF2, SlCDF3, SlCDF4, and SlCDF5 are homologs to Arabidopsis cycling DOF factor 1 (AtCDF1). SlCDF1–5 expression levels were influenced by the developmental stage and the tissue location, and notably, the expression patterns throughout light environments showed two opposite trends. Among the SlCDF1–5 overexpression transgenic lines, overexpressing SlCDF3 delayed flowering time in both LD (16 h light/8 h dark) and SD (8 h light/16 h dark) conditions. Furthermore, SlCDF3 led to an increase in the mRNA level of SlSP5G, a tomato FT-like gene, in LD conditions, while the transcription level of the other two FT-like genes, SlSP5G2 and SlSP5G3, were up-regulated in SD conditions. Taken together, at the transcription level, our results demonstrated that SlCDF3 played a significant role in controlling tomato flowering under LD and SD conditions, possibly through directly or indirectly regulating FT-like genes. Frontiers Media S.A. 2021-05-05 /pmc/articles/PMC8131850/ /pubmed/34025696 http://dx.doi.org/10.3389/fpls.2021.650068 Text en Copyright © 2021 Xu, Li, Wu, Meng, Zou, Bao, Bian and Cao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Xu, Dawei
Li, Xueou
Wu, Xue
Meng, Lili
Zou, Zhirong
Bao, Encai
Bian, Zhonghua
Cao, Kai
Tomato SlCDF3 Delays Flowering Time by Regulating Different FT-Like Genes Under Long-Day and Short-Day Conditions
title Tomato SlCDF3 Delays Flowering Time by Regulating Different FT-Like Genes Under Long-Day and Short-Day Conditions
title_full Tomato SlCDF3 Delays Flowering Time by Regulating Different FT-Like Genes Under Long-Day and Short-Day Conditions
title_fullStr Tomato SlCDF3 Delays Flowering Time by Regulating Different FT-Like Genes Under Long-Day and Short-Day Conditions
title_full_unstemmed Tomato SlCDF3 Delays Flowering Time by Regulating Different FT-Like Genes Under Long-Day and Short-Day Conditions
title_short Tomato SlCDF3 Delays Flowering Time by Regulating Different FT-Like Genes Under Long-Day and Short-Day Conditions
title_sort tomato slcdf3 delays flowering time by regulating different ft-like genes under long-day and short-day conditions
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131850/
https://www.ncbi.nlm.nih.gov/pubmed/34025696
http://dx.doi.org/10.3389/fpls.2021.650068
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