Dynamic BH3 profiling method for rapid identification of active therapy in BH3 mimetics resistant xenograft mouse models

The clinical effectiveness of BH3 mimetics therapy is limited by the inevitable emergence of acquired resistance. We present a protocol to model in vivo acquired resistance to BH3 mimetics in patient-derived xenograft (PDX) mouse models of acute myeloid leukemia. Using resistant PDXs as a valuable m...

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Detalles Bibliográficos
Autores principales: Olesinski, Elyse A., Bhatt, Shruti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131978/
https://www.ncbi.nlm.nih.gov/pubmed/34027474
http://dx.doi.org/10.1016/j.xpro.2021.100461
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author Olesinski, Elyse A.
Bhatt, Shruti
author_facet Olesinski, Elyse A.
Bhatt, Shruti
author_sort Olesinski, Elyse A.
collection PubMed
description The clinical effectiveness of BH3 mimetics therapy is limited by the inevitable emergence of acquired resistance. We present a protocol to model in vivo acquired resistance to BH3 mimetics in patient-derived xenograft (PDX) mouse models of acute myeloid leukemia. Using resistant PDXs as a valuable model, we next introduce a protocol for dynamic BH3 profiling (DBP) method. DBP allows functional identification of effective drug therapies based on measurements of drug-induced apoptosis signaling to overcome in vivo BH3 mimetics resistance. For complete details on the use and execution of this protocol, please refer to Bhatt et al. (2020).
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spelling pubmed-81319782021-05-21 Dynamic BH3 profiling method for rapid identification of active therapy in BH3 mimetics resistant xenograft mouse models Olesinski, Elyse A. Bhatt, Shruti STAR Protoc Protocol The clinical effectiveness of BH3 mimetics therapy is limited by the inevitable emergence of acquired resistance. We present a protocol to model in vivo acquired resistance to BH3 mimetics in patient-derived xenograft (PDX) mouse models of acute myeloid leukemia. Using resistant PDXs as a valuable model, we next introduce a protocol for dynamic BH3 profiling (DBP) method. DBP allows functional identification of effective drug therapies based on measurements of drug-induced apoptosis signaling to overcome in vivo BH3 mimetics resistance. For complete details on the use and execution of this protocol, please refer to Bhatt et al. (2020). Elsevier 2021-05-12 /pmc/articles/PMC8131978/ /pubmed/34027474 http://dx.doi.org/10.1016/j.xpro.2021.100461 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Olesinski, Elyse A.
Bhatt, Shruti
Dynamic BH3 profiling method for rapid identification of active therapy in BH3 mimetics resistant xenograft mouse models
title Dynamic BH3 profiling method for rapid identification of active therapy in BH3 mimetics resistant xenograft mouse models
title_full Dynamic BH3 profiling method for rapid identification of active therapy in BH3 mimetics resistant xenograft mouse models
title_fullStr Dynamic BH3 profiling method for rapid identification of active therapy in BH3 mimetics resistant xenograft mouse models
title_full_unstemmed Dynamic BH3 profiling method for rapid identification of active therapy in BH3 mimetics resistant xenograft mouse models
title_short Dynamic BH3 profiling method for rapid identification of active therapy in BH3 mimetics resistant xenograft mouse models
title_sort dynamic bh3 profiling method for rapid identification of active therapy in bh3 mimetics resistant xenograft mouse models
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8131978/
https://www.ncbi.nlm.nih.gov/pubmed/34027474
http://dx.doi.org/10.1016/j.xpro.2021.100461
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