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Spatial multi-omics sequencing for fixed tissue via DBiT-seq
This protocol describes the use of the deterministic barcoding in tissue for spatial omics sequencing platform to construct a multi-omics atlas on fixed frozen tissue samples. This approach uses a microfluidic-based method to introduce combinatorial DNA oligo barcodes directly to the cells in a tiss...
Autores principales: | Su, Graham, Qin, Xiaoyu, Enninful, Archibald, Bai, Zhiliang, Deng, Yanxiang, Liu, Yang, Fan, Rong |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132129/ https://www.ncbi.nlm.nih.gov/pubmed/34027489 http://dx.doi.org/10.1016/j.xpro.2021.100532 |
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