Single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression

This work describes a microfluidic system for in situ extraction of a single-cell and its phosphatidylcholine analysis through mass spectrometry. This approach uncovered cellular heterogeneity among seemingly identical cells and provided a new platform for identification and classification of cells.

Detalles Bibliográficos
Autores principales: Huang, Qiushi, Mao, Sifeng, Khan, Mashooq, Li, Weiwei, Zhang, Qiang, Lin, Jin-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132990/
https://www.ncbi.nlm.nih.gov/pubmed/34040719
http://dx.doi.org/10.1039/c9sc05143k
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author Huang, Qiushi
Mao, Sifeng
Khan, Mashooq
Li, Weiwei
Zhang, Qiang
Lin, Jin-Ming
author_facet Huang, Qiushi
Mao, Sifeng
Khan, Mashooq
Li, Weiwei
Zhang, Qiang
Lin, Jin-Ming
author_sort Huang, Qiushi
collection PubMed
description This work describes a microfluidic system for in situ extraction of a single-cell and its phosphatidylcholine analysis through mass spectrometry. This approach uncovered cellular heterogeneity among seemingly identical cells and provided a new platform for identification and classification of cells.
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spelling pubmed-81329902021-05-25 Single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression Huang, Qiushi Mao, Sifeng Khan, Mashooq Li, Weiwei Zhang, Qiang Lin, Jin-Ming Chem Sci Chemistry This work describes a microfluidic system for in situ extraction of a single-cell and its phosphatidylcholine analysis through mass spectrometry. This approach uncovered cellular heterogeneity among seemingly identical cells and provided a new platform for identification and classification of cells. The Royal Society of Chemistry 2019-11-11 /pmc/articles/PMC8132990/ /pubmed/34040719 http://dx.doi.org/10.1039/c9sc05143k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Huang, Qiushi
Mao, Sifeng
Khan, Mashooq
Li, Weiwei
Zhang, Qiang
Lin, Jin-Ming
Single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression
title Single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression
title_full Single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression
title_fullStr Single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression
title_full_unstemmed Single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression
title_short Single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression
title_sort single-cell identification by microfluidic-based in situ extracting and online mass spectrometric analysis of phospholipids expression
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132990/
https://www.ncbi.nlm.nih.gov/pubmed/34040719
http://dx.doi.org/10.1039/c9sc05143k
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AT maosifeng singlecellidentificationbymicrofluidicbasedinsituextractingandonlinemassspectrometricanalysisofphospholipidsexpression
AT khanmashooq singlecellidentificationbymicrofluidicbasedinsituextractingandonlinemassspectrometricanalysisofphospholipidsexpression
AT liweiwei singlecellidentificationbymicrofluidicbasedinsituextractingandonlinemassspectrometricanalysisofphospholipidsexpression
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