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Light-driven release of cucurbit[8]uril from a bivalent cage
Temporal control over supramolecular systems has great potential for the modulation of binding and assembly events, such as providing orthogonal control over protein activity. Especially light controlled triggering provides unique entries for supramolecular systems to interface in a controlled manne...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132991/ https://www.ncbi.nlm.nih.gov/pubmed/34040748 http://dx.doi.org/10.1039/d1sc01410b |
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author | de Vink, Pim J. van der Hek, Tim Brunsveld, Luc |
author_facet | de Vink, Pim J. van der Hek, Tim Brunsveld, Luc |
author_sort | de Vink, Pim J. |
collection | PubMed |
description | Temporal control over supramolecular systems has great potential for the modulation of binding and assembly events, such as providing orthogonal control over protein activity. Especially light controlled triggering provides unique entries for supramolecular systems to interface in a controlled manner with enzymes. Here we report on the light-induced release of cucurbit[8]uril (CB[8]) from a bivalent cage molecule and its subsequent activation of a proteolytic enzyme, caspase-9, that itself is unresponsive to light. Central to the design is the bivalent binding of the cage with high affinity to CB[8], 100-fold stronger than the UV-inactivated products. The affinity switching occurs in the (sub-)micromolar concentration regime, matching the concentration characteristics required for dimerizing and activating caspase-9 by CB[8]. The light-responsive caged CB[8] concept presented offers a novel platform for tuning and application of switchable cucurbiturils and beyond. |
format | Online Article Text |
id | pubmed-8132991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-81329912021-05-25 Light-driven release of cucurbit[8]uril from a bivalent cage de Vink, Pim J. van der Hek, Tim Brunsveld, Luc Chem Sci Chemistry Temporal control over supramolecular systems has great potential for the modulation of binding and assembly events, such as providing orthogonal control over protein activity. Especially light controlled triggering provides unique entries for supramolecular systems to interface in a controlled manner with enzymes. Here we report on the light-induced release of cucurbit[8]uril (CB[8]) from a bivalent cage molecule and its subsequent activation of a proteolytic enzyme, caspase-9, that itself is unresponsive to light. Central to the design is the bivalent binding of the cage with high affinity to CB[8], 100-fold stronger than the UV-inactivated products. The affinity switching occurs in the (sub-)micromolar concentration regime, matching the concentration characteristics required for dimerizing and activating caspase-9 by CB[8]. The light-responsive caged CB[8] concept presented offers a novel platform for tuning and application of switchable cucurbiturils and beyond. The Royal Society of Chemistry 2021-04-12 /pmc/articles/PMC8132991/ /pubmed/34040748 http://dx.doi.org/10.1039/d1sc01410b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry de Vink, Pim J. van der Hek, Tim Brunsveld, Luc Light-driven release of cucurbit[8]uril from a bivalent cage |
title | Light-driven release of cucurbit[8]uril from a bivalent cage |
title_full | Light-driven release of cucurbit[8]uril from a bivalent cage |
title_fullStr | Light-driven release of cucurbit[8]uril from a bivalent cage |
title_full_unstemmed | Light-driven release of cucurbit[8]uril from a bivalent cage |
title_short | Light-driven release of cucurbit[8]uril from a bivalent cage |
title_sort | light-driven release of cucurbit[8]uril from a bivalent cage |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8132991/ https://www.ncbi.nlm.nih.gov/pubmed/34040748 http://dx.doi.org/10.1039/d1sc01410b |
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