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Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge

Dynamic changes in flavonoid, total phenol, and antioxidant potential in different Prunus humilis accessions during fruit development stages were studied in order to provide a reference for the optimum harvest time for flavonoid extraction. ‘Nongda 4’, ‘Nongda 5’, ‘DS-1’ and ‘02–16’ were selected as...

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Autores principales: Fu, Hongbo, Qiao, Yujia, Wang, Pengfei, Mu, Xiaopeng, Zhang, Jiancheng, Fu, Baochun, Du, Junjie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133403/
https://www.ncbi.nlm.nih.gov/pubmed/34010329
http://dx.doi.org/10.1371/journal.pone.0251300
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author Fu, Hongbo
Qiao, Yujia
Wang, Pengfei
Mu, Xiaopeng
Zhang, Jiancheng
Fu, Baochun
Du, Junjie
author_facet Fu, Hongbo
Qiao, Yujia
Wang, Pengfei
Mu, Xiaopeng
Zhang, Jiancheng
Fu, Baochun
Du, Junjie
author_sort Fu, Hongbo
collection PubMed
description Dynamic changes in flavonoid, total phenol, and antioxidant potential in different Prunus humilis accessions during fruit development stages were studied in order to provide a reference for the optimum harvest time for flavonoid extraction. ‘Nongda 4’, ‘Nongda 5’, ‘DS-1’ and ‘02–16’ were selected as plant materials to determine the content of flavonoid, total phenol and antioxidant indices during six fruit development stages. Changes in total flavonoid content (TFC) and total phenol content (TPC) in different accessions of P. humilis were slightly different depending on the development stage of P. humilis fruit. TFC and TPC in ‘Nongda 5’ fruit showed a trend of continuous decline. There was a small increase in TFC and TPC from the young fruit stage to the stone hardening stage, followed by a decreasing trend, and then to the lowest level at the ripening stage of ‘Nongda 4’, ‘DS-1’, and ‘02–16’ fruits. The trend of antioxidant capacity (ABTS, FRAP, DPPH) with the TFC and TPC of P. humilis fruit was basically the same, and the correlation analysis results showed that the TFC of P. humilis fruit was positively correlated with the antioxidant indices (P<0.01). Catechin (CC), rutin (RT), and quercetin-7-O-β-D-glucopyranoside (Q7G) were detected in all the fruit development stages of the four P. humilis fruits. Among them, catechin was the most abundant component, accounting for approximately 10%. Myricetin (MC) and quercetin (QC) were generally detected only in the early fruit development stage, but not in the later fruit development stage. Correlation analysis showed that the flavonoid components with TFC, TPC, and antioxidant indices differed between the different accessions. RT, CC, and liquiritigenin (LR) had a stronger correlation with TFC and antioxidant indices. Cyanidin-3-O-glucoside (C3G) was not detected until the coloring stage in two red P. humilis accessions (‘Nongda 4’ and ‘DS-1’), and so it is better to choose a red P. humilis fruit to extract C3G at the ripening stage. Selecting an early stage of fruit development, especially the stone hardening stage, was important for extracting flavonoids, total phenols and other components. We believe that our results will provide basic information and reference for evaluation of fruit nutrition and health benefits, breeding of functional new varieties, and efficient utilization of P. humilis fruit.
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spelling pubmed-81334032021-05-27 Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge Fu, Hongbo Qiao, Yujia Wang, Pengfei Mu, Xiaopeng Zhang, Jiancheng Fu, Baochun Du, Junjie PLoS One Research Article Dynamic changes in flavonoid, total phenol, and antioxidant potential in different Prunus humilis accessions during fruit development stages were studied in order to provide a reference for the optimum harvest time for flavonoid extraction. ‘Nongda 4’, ‘Nongda 5’, ‘DS-1’ and ‘02–16’ were selected as plant materials to determine the content of flavonoid, total phenol and antioxidant indices during six fruit development stages. Changes in total flavonoid content (TFC) and total phenol content (TPC) in different accessions of P. humilis were slightly different depending on the development stage of P. humilis fruit. TFC and TPC in ‘Nongda 5’ fruit showed a trend of continuous decline. There was a small increase in TFC and TPC from the young fruit stage to the stone hardening stage, followed by a decreasing trend, and then to the lowest level at the ripening stage of ‘Nongda 4’, ‘DS-1’, and ‘02–16’ fruits. The trend of antioxidant capacity (ABTS, FRAP, DPPH) with the TFC and TPC of P. humilis fruit was basically the same, and the correlation analysis results showed that the TFC of P. humilis fruit was positively correlated with the antioxidant indices (P<0.01). Catechin (CC), rutin (RT), and quercetin-7-O-β-D-glucopyranoside (Q7G) were detected in all the fruit development stages of the four P. humilis fruits. Among them, catechin was the most abundant component, accounting for approximately 10%. Myricetin (MC) and quercetin (QC) were generally detected only in the early fruit development stage, but not in the later fruit development stage. Correlation analysis showed that the flavonoid components with TFC, TPC, and antioxidant indices differed between the different accessions. RT, CC, and liquiritigenin (LR) had a stronger correlation with TFC and antioxidant indices. Cyanidin-3-O-glucoside (C3G) was not detected until the coloring stage in two red P. humilis accessions (‘Nongda 4’ and ‘DS-1’), and so it is better to choose a red P. humilis fruit to extract C3G at the ripening stage. Selecting an early stage of fruit development, especially the stone hardening stage, was important for extracting flavonoids, total phenols and other components. We believe that our results will provide basic information and reference for evaluation of fruit nutrition and health benefits, breeding of functional new varieties, and efficient utilization of P. humilis fruit. Public Library of Science 2021-05-19 /pmc/articles/PMC8133403/ /pubmed/34010329 http://dx.doi.org/10.1371/journal.pone.0251300 Text en © 2021 Fu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fu, Hongbo
Qiao, Yujia
Wang, Pengfei
Mu, Xiaopeng
Zhang, Jiancheng
Fu, Baochun
Du, Junjie
Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge
title Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge
title_full Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge
title_fullStr Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge
title_full_unstemmed Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge
title_short Changes of bioactive components and antioxidant potential during fruit development of Prunus humilis Bunge
title_sort changes of bioactive components and antioxidant potential during fruit development of prunus humilis bunge
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133403/
https://www.ncbi.nlm.nih.gov/pubmed/34010329
http://dx.doi.org/10.1371/journal.pone.0251300
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