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PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula

Legumes play an important role in the soil nitrogen availability via symbiotic nitrogen fixation (SNF). Phosphate (Pi) deficiency severely impacts SNF because of the high Pi requirement of symbiosis. Whereas PHT1 transporters are involved in Pi uptake into nodules, it is unknown how Pi is transferre...

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Autores principales: Nguyen, Nga N T, Clua, Joaquin, Vetal, Pallavi V, Vuarambon, Dominique Jacques, De Bellis, Damien, Pervent, Marjorie, Lepetit, Marc, Udvardi, Michael, Valentine, Alexander J, Poirier, Yves
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133656/
https://www.ncbi.nlm.nih.gov/pubmed/33631809
http://dx.doi.org/10.1093/plphys/kiaa016
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author Nguyen, Nga N T
Clua, Joaquin
Vetal, Pallavi V
Vuarambon, Dominique Jacques
De Bellis, Damien
Pervent, Marjorie
Lepetit, Marc
Udvardi, Michael
Valentine, Alexander J
Poirier, Yves
author_facet Nguyen, Nga N T
Clua, Joaquin
Vetal, Pallavi V
Vuarambon, Dominique Jacques
De Bellis, Damien
Pervent, Marjorie
Lepetit, Marc
Udvardi, Michael
Valentine, Alexander J
Poirier, Yves
author_sort Nguyen, Nga N T
collection PubMed
description Legumes play an important role in the soil nitrogen availability via symbiotic nitrogen fixation (SNF). Phosphate (Pi) deficiency severely impacts SNF because of the high Pi requirement of symbiosis. Whereas PHT1 transporters are involved in Pi uptake into nodules, it is unknown how Pi is transferred from the plant infected cells to nitrogen-fixing bacteroids. We hypothesized that Medicago truncatula genes homologous to Arabidopsis PHO1, encoding a vascular apoplastic Pi exporter, are involved in Pi transfer to bacteroids. Among the seven MtPHO1 genes present in M. truncatula, we found that two genes, namely MtPHO1.1 and MtPHO1.2, were broadly expressed across the various nodule zones in addition to the root vascular system. Expressions of MtPHO1.1 and MtPHO1.2 in Nicotiana benthamiana mediated specific Pi export. Plants with nodule-specific downregulation of both MtPHO1.1 and MtPHO1.2 were generated by RNA interference (RNAi) to examine their roles in nodule Pi homeostasis. Nodules of RNAi plants had lower Pi content and a three-fold reduction in SNF, resulting in reduced shoot growth. Whereas the rate of (33)Pi uptake into nodules of RNAi plants was similar to control, transfer of (33)Pi from nodule cells into bacteroids was reduced and bacteroids activated their Pi-deficiency response. Our results implicate plant MtPHO1 genes in bacteroid Pi homeostasis and SNF via the transfer of Pi from nodule infected cells to bacteroids.
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spelling pubmed-81336562021-05-25 PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula Nguyen, Nga N T Clua, Joaquin Vetal, Pallavi V Vuarambon, Dominique Jacques De Bellis, Damien Pervent, Marjorie Lepetit, Marc Udvardi, Michael Valentine, Alexander J Poirier, Yves Plant Physiol Research Articles Legumes play an important role in the soil nitrogen availability via symbiotic nitrogen fixation (SNF). Phosphate (Pi) deficiency severely impacts SNF because of the high Pi requirement of symbiosis. Whereas PHT1 transporters are involved in Pi uptake into nodules, it is unknown how Pi is transferred from the plant infected cells to nitrogen-fixing bacteroids. We hypothesized that Medicago truncatula genes homologous to Arabidopsis PHO1, encoding a vascular apoplastic Pi exporter, are involved in Pi transfer to bacteroids. Among the seven MtPHO1 genes present in M. truncatula, we found that two genes, namely MtPHO1.1 and MtPHO1.2, were broadly expressed across the various nodule zones in addition to the root vascular system. Expressions of MtPHO1.1 and MtPHO1.2 in Nicotiana benthamiana mediated specific Pi export. Plants with nodule-specific downregulation of both MtPHO1.1 and MtPHO1.2 were generated by RNA interference (RNAi) to examine their roles in nodule Pi homeostasis. Nodules of RNAi plants had lower Pi content and a three-fold reduction in SNF, resulting in reduced shoot growth. Whereas the rate of (33)Pi uptake into nodules of RNAi plants was similar to control, transfer of (33)Pi from nodule cells into bacteroids was reduced and bacteroids activated their Pi-deficiency response. Our results implicate plant MtPHO1 genes in bacteroid Pi homeostasis and SNF via the transfer of Pi from nodule infected cells to bacteroids. Oxford University Press 2020-11-25 /pmc/articles/PMC8133656/ /pubmed/33631809 http://dx.doi.org/10.1093/plphys/kiaa016 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Articles
Nguyen, Nga N T
Clua, Joaquin
Vetal, Pallavi V
Vuarambon, Dominique Jacques
De Bellis, Damien
Pervent, Marjorie
Lepetit, Marc
Udvardi, Michael
Valentine, Alexander J
Poirier, Yves
PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula
title PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula
title_full PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula
title_fullStr PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula
title_full_unstemmed PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula
title_short PHO1 family members transport phosphate from infected nodule cells to bacteroids in Medicago truncatula
title_sort pho1 family members transport phosphate from infected nodule cells to bacteroids in medicago truncatula
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133656/
https://www.ncbi.nlm.nih.gov/pubmed/33631809
http://dx.doi.org/10.1093/plphys/kiaa016
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