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Root vacuolar sequestration and suberization are prominent responses of Pistacia spp. rootstocks during salinity stress

Understanding the mechanisms of stress tolerance in diverse species is needed to enhance crop performance under conditions such as high salinity. Plant roots, in particular in grafted agricultural crops, can function as a boundary against external stresses in order to maintain plant fitness. However...

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Autores principales: Zhang, Shuxiao, Quartararo, Alessandra, Betz, Oliver Karl, Madahhosseini, Shahab, Heringer, Angelo Schuabb, Le, Thu, Shao, Yuhang, Caruso, Tiziano, Ferguson, Louise, Jernstedt, Judy, Wilkop, Thomas, Drakakaki, Georgia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133763/
https://www.ncbi.nlm.nih.gov/pubmed/34027297
http://dx.doi.org/10.1002/pld3.315
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author Zhang, Shuxiao
Quartararo, Alessandra
Betz, Oliver Karl
Madahhosseini, Shahab
Heringer, Angelo Schuabb
Le, Thu
Shao, Yuhang
Caruso, Tiziano
Ferguson, Louise
Jernstedt, Judy
Wilkop, Thomas
Drakakaki, Georgia
author_facet Zhang, Shuxiao
Quartararo, Alessandra
Betz, Oliver Karl
Madahhosseini, Shahab
Heringer, Angelo Schuabb
Le, Thu
Shao, Yuhang
Caruso, Tiziano
Ferguson, Louise
Jernstedt, Judy
Wilkop, Thomas
Drakakaki, Georgia
author_sort Zhang, Shuxiao
collection PubMed
description Understanding the mechanisms of stress tolerance in diverse species is needed to enhance crop performance under conditions such as high salinity. Plant roots, in particular in grafted agricultural crops, can function as a boundary against external stresses in order to maintain plant fitness. However, limited information exists for salinity stress responses of woody species and their rootstocks. Pistachio (Pistacia spp.) is a tree nut crop with relatively high salinity tolerance as well as high genetic heterogeneity. In this study, we used a microscopy‐based approach to investigate the cellular and structural responses to salinity stress in the roots of two pistachio rootstocks, Pistacia integerrima (PGI) and a hybrid, P. atlantica x P. integerrima (UCB1). We analyzed root sections via fluorescence microscopy across a developmental gradient, defined by xylem development, for sodium localization and for cellular barrier differentiation via suberin deposition. Our cumulative data suggest that the salinity response in pistachio rootstock species is associated with both vacuolar sodium ion (Na(+)) sequestration in the root cortex and increased suberin deposition at apoplastic barriers. Furthermore, both vacuolar sequestration and suberin deposition correlate with the root developmental gradient. We observed a higher rate of Na(+) vacuolar sequestration and reduced salt‐induced leaf damage in UCB1 when compared to P. integerrima. In addition, UCB1 displayed higher basal levels of suberization, in both the exodermis and endodermis, compared to P. integerrima. This difference was enhanced after salinity stress. These cellular characteristics are phenotypes that can be taken into account during screening for sodium‐mediated salinity tolerance in woody plant species.
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spelling pubmed-81337632021-05-21 Root vacuolar sequestration and suberization are prominent responses of Pistacia spp. rootstocks during salinity stress Zhang, Shuxiao Quartararo, Alessandra Betz, Oliver Karl Madahhosseini, Shahab Heringer, Angelo Schuabb Le, Thu Shao, Yuhang Caruso, Tiziano Ferguson, Louise Jernstedt, Judy Wilkop, Thomas Drakakaki, Georgia Plant Direct Original Research Understanding the mechanisms of stress tolerance in diverse species is needed to enhance crop performance under conditions such as high salinity. Plant roots, in particular in grafted agricultural crops, can function as a boundary against external stresses in order to maintain plant fitness. However, limited information exists for salinity stress responses of woody species and their rootstocks. Pistachio (Pistacia spp.) is a tree nut crop with relatively high salinity tolerance as well as high genetic heterogeneity. In this study, we used a microscopy‐based approach to investigate the cellular and structural responses to salinity stress in the roots of two pistachio rootstocks, Pistacia integerrima (PGI) and a hybrid, P. atlantica x P. integerrima (UCB1). We analyzed root sections via fluorescence microscopy across a developmental gradient, defined by xylem development, for sodium localization and for cellular barrier differentiation via suberin deposition. Our cumulative data suggest that the salinity response in pistachio rootstock species is associated with both vacuolar sodium ion (Na(+)) sequestration in the root cortex and increased suberin deposition at apoplastic barriers. Furthermore, both vacuolar sequestration and suberin deposition correlate with the root developmental gradient. We observed a higher rate of Na(+) vacuolar sequestration and reduced salt‐induced leaf damage in UCB1 when compared to P. integerrima. In addition, UCB1 displayed higher basal levels of suberization, in both the exodermis and endodermis, compared to P. integerrima. This difference was enhanced after salinity stress. These cellular characteristics are phenotypes that can be taken into account during screening for sodium‐mediated salinity tolerance in woody plant species. John Wiley and Sons Inc. 2021-05-19 /pmc/articles/PMC8133763/ /pubmed/34027297 http://dx.doi.org/10.1002/pld3.315 Text en © 2021 The Authors. Plant Direct published by American Society of Plant Biologists, Society for Experimental Biology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Research
Zhang, Shuxiao
Quartararo, Alessandra
Betz, Oliver Karl
Madahhosseini, Shahab
Heringer, Angelo Schuabb
Le, Thu
Shao, Yuhang
Caruso, Tiziano
Ferguson, Louise
Jernstedt, Judy
Wilkop, Thomas
Drakakaki, Georgia
Root vacuolar sequestration and suberization are prominent responses of Pistacia spp. rootstocks during salinity stress
title Root vacuolar sequestration and suberization are prominent responses of Pistacia spp. rootstocks during salinity stress
title_full Root vacuolar sequestration and suberization are prominent responses of Pistacia spp. rootstocks during salinity stress
title_fullStr Root vacuolar sequestration and suberization are prominent responses of Pistacia spp. rootstocks during salinity stress
title_full_unstemmed Root vacuolar sequestration and suberization are prominent responses of Pistacia spp. rootstocks during salinity stress
title_short Root vacuolar sequestration and suberization are prominent responses of Pistacia spp. rootstocks during salinity stress
title_sort root vacuolar sequestration and suberization are prominent responses of pistacia spp. rootstocks during salinity stress
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133763/
https://www.ncbi.nlm.nih.gov/pubmed/34027297
http://dx.doi.org/10.1002/pld3.315
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