Cargando…

Longitudinal [(18)F]FDG and [(13)N]NH(3) PET/CT imaging of brain and spinal cord in a canine hemisection spinal cord injury model

To further understand the neurological changes induced by spinal cord injury (SCI) in its acute and subacute stages, we evaluated longitudinal changes in glucose and glutamate metabolism in the spinal cord and brain regions of a canine hemisection SCI model. [(18)F]FDG and [(13)N]NH(3) positron-emis...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Lijian, López-Picón, Francisco R., Jia, Yingqin, Chen, Yao, Li, Juan, Han, Chunlei, Zhuang, Xiaoqing, Xia, Hechun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134064/
https://www.ncbi.nlm.nih.gov/pubmed/33992987
http://dx.doi.org/10.1016/j.nicl.2021.102692
_version_ 1783695158199451648
author Zhang, Lijian
López-Picón, Francisco R.
Jia, Yingqin
Chen, Yao
Li, Juan
Han, Chunlei
Zhuang, Xiaoqing
Xia, Hechun
author_facet Zhang, Lijian
López-Picón, Francisco R.
Jia, Yingqin
Chen, Yao
Li, Juan
Han, Chunlei
Zhuang, Xiaoqing
Xia, Hechun
author_sort Zhang, Lijian
collection PubMed
description To further understand the neurological changes induced by spinal cord injury (SCI) in its acute and subacute stages, we evaluated longitudinal changes in glucose and glutamate metabolism in the spinal cord and brain regions of a canine hemisection SCI model. [(18)F]FDG and [(13)N]NH(3) positron-emission tomography (PET) with computed tomography (CT) was performed before SCI and at 1, 3, 7, 14, and 21 days after SCI. Spinal cord [(18)F]FDG uptake increased and peaked at 3 days post SCI. Similar changes were observed in the brain regions but were not statistically significant. Compared to the acute phase of SCI, [(13)N]NH(3) uptake increased in the subacute stage and peaked at 7 days post SCI in all analyzed brain regions. But in spinal cord, no [(13)N]NH(3) uptake was detected before SCI when the blood-spinal cord barrier (BSCB) was intact, then gradually increased when the BSCB was damaged after SCI. [(13)N]NH(3) uptake was significantly correlated with plasma levels of the BSCB disruption marker, monocyte chemoattractant protein-1 (MCP-1). Overall, we showed that SCI induced in vivo changes in glucose uptake in both the spinal cord and the examined brain regions, and changes in glutamine synthetase activity in the latter. Moreover, our results suggest that [(13)N]NH(3) PET may serve as a potential method for assessing BSCB permeability in vivo.
format Online
Article
Text
id pubmed-8134064
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-81340642021-05-21 Longitudinal [(18)F]FDG and [(13)N]NH(3) PET/CT imaging of brain and spinal cord in a canine hemisection spinal cord injury model Zhang, Lijian López-Picón, Francisco R. Jia, Yingqin Chen, Yao Li, Juan Han, Chunlei Zhuang, Xiaoqing Xia, Hechun Neuroimage Clin Regular Article To further understand the neurological changes induced by spinal cord injury (SCI) in its acute and subacute stages, we evaluated longitudinal changes in glucose and glutamate metabolism in the spinal cord and brain regions of a canine hemisection SCI model. [(18)F]FDG and [(13)N]NH(3) positron-emission tomography (PET) with computed tomography (CT) was performed before SCI and at 1, 3, 7, 14, and 21 days after SCI. Spinal cord [(18)F]FDG uptake increased and peaked at 3 days post SCI. Similar changes were observed in the brain regions but were not statistically significant. Compared to the acute phase of SCI, [(13)N]NH(3) uptake increased in the subacute stage and peaked at 7 days post SCI in all analyzed brain regions. But in spinal cord, no [(13)N]NH(3) uptake was detected before SCI when the blood-spinal cord barrier (BSCB) was intact, then gradually increased when the BSCB was damaged after SCI. [(13)N]NH(3) uptake was significantly correlated with plasma levels of the BSCB disruption marker, monocyte chemoattractant protein-1 (MCP-1). Overall, we showed that SCI induced in vivo changes in glucose uptake in both the spinal cord and the examined brain regions, and changes in glutamine synthetase activity in the latter. Moreover, our results suggest that [(13)N]NH(3) PET may serve as a potential method for assessing BSCB permeability in vivo. Elsevier 2021-05-04 /pmc/articles/PMC8134064/ /pubmed/33992987 http://dx.doi.org/10.1016/j.nicl.2021.102692 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Regular Article
Zhang, Lijian
López-Picón, Francisco R.
Jia, Yingqin
Chen, Yao
Li, Juan
Han, Chunlei
Zhuang, Xiaoqing
Xia, Hechun
Longitudinal [(18)F]FDG and [(13)N]NH(3) PET/CT imaging of brain and spinal cord in a canine hemisection spinal cord injury model
title Longitudinal [(18)F]FDG and [(13)N]NH(3) PET/CT imaging of brain and spinal cord in a canine hemisection spinal cord injury model
title_full Longitudinal [(18)F]FDG and [(13)N]NH(3) PET/CT imaging of brain and spinal cord in a canine hemisection spinal cord injury model
title_fullStr Longitudinal [(18)F]FDG and [(13)N]NH(3) PET/CT imaging of brain and spinal cord in a canine hemisection spinal cord injury model
title_full_unstemmed Longitudinal [(18)F]FDG and [(13)N]NH(3) PET/CT imaging of brain and spinal cord in a canine hemisection spinal cord injury model
title_short Longitudinal [(18)F]FDG and [(13)N]NH(3) PET/CT imaging of brain and spinal cord in a canine hemisection spinal cord injury model
title_sort longitudinal [(18)f]fdg and [(13)n]nh(3) pet/ct imaging of brain and spinal cord in a canine hemisection spinal cord injury model
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134064/
https://www.ncbi.nlm.nih.gov/pubmed/33992987
http://dx.doi.org/10.1016/j.nicl.2021.102692
work_keys_str_mv AT zhanglijian longitudinal18ffdgand13nnh3petctimagingofbrainandspinalcordinacaninehemisectionspinalcordinjurymodel
AT lopezpiconfranciscor longitudinal18ffdgand13nnh3petctimagingofbrainandspinalcordinacaninehemisectionspinalcordinjurymodel
AT jiayingqin longitudinal18ffdgand13nnh3petctimagingofbrainandspinalcordinacaninehemisectionspinalcordinjurymodel
AT chenyao longitudinal18ffdgand13nnh3petctimagingofbrainandspinalcordinacaninehemisectionspinalcordinjurymodel
AT lijuan longitudinal18ffdgand13nnh3petctimagingofbrainandspinalcordinacaninehemisectionspinalcordinjurymodel
AT hanchunlei longitudinal18ffdgand13nnh3petctimagingofbrainandspinalcordinacaninehemisectionspinalcordinjurymodel
AT zhuangxiaoqing longitudinal18ffdgand13nnh3petctimagingofbrainandspinalcordinacaninehemisectionspinalcordinjurymodel
AT xiahechun longitudinal18ffdgand13nnh3petctimagingofbrainandspinalcordinacaninehemisectionspinalcordinjurymodel