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Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography

Root water uptake is an essential process for terrestrial plants that strongly affects the spatiotemporal distribution of water in vegetated soil. Fast neutron tomography is a recently established non-invasive imaging technique capable to capture the 3D architecture of root systems in situ and even...

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Autores principales: Tötzke, Christian, Kardjilov, Nikolay, Hilger, André, Rudolph-Mohr, Nicole, Manke, Ingo, Oswald, Sascha E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134433/
https://www.ncbi.nlm.nih.gov/pubmed/34012044
http://dx.doi.org/10.1038/s41598-021-90062-4
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author Tötzke, Christian
Kardjilov, Nikolay
Hilger, André
Rudolph-Mohr, Nicole
Manke, Ingo
Oswald, Sascha E.
author_facet Tötzke, Christian
Kardjilov, Nikolay
Hilger, André
Rudolph-Mohr, Nicole
Manke, Ingo
Oswald, Sascha E.
author_sort Tötzke, Christian
collection PubMed
description Root water uptake is an essential process for terrestrial plants that strongly affects the spatiotemporal distribution of water in vegetated soil. Fast neutron tomography is a recently established non-invasive imaging technique capable to capture the 3D architecture of root systems in situ and even allows for tracking of three-dimensional water flow in soil and roots. We present an in vivo analysis of local water uptake and transport by roots of soil-grown maize plants—for the first time measured in a three-dimensional time-resolved manner. Using deuterated water as tracer in infiltration experiments, we visualized soil imbibition, local root uptake, and tracked the transport of deuterated water throughout the fibrous root system for a day and night situation. This revealed significant differences in water transport between different root types. The primary root was the preferred water transport path in the 13-days-old plants while seminal roots of comparable size and length contributed little to plant water supply. The results underline the unique potential of fast neutron tomography to provide time-resolved 3D in vivo information on the water uptake and transport dynamics of plant root systems, thus contributing to a better understanding of the complex interactions of plant, soil and water.
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spelling pubmed-81344332021-05-25 Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography Tötzke, Christian Kardjilov, Nikolay Hilger, André Rudolph-Mohr, Nicole Manke, Ingo Oswald, Sascha E. Sci Rep Article Root water uptake is an essential process for terrestrial plants that strongly affects the spatiotemporal distribution of water in vegetated soil. Fast neutron tomography is a recently established non-invasive imaging technique capable to capture the 3D architecture of root systems in situ and even allows for tracking of three-dimensional water flow in soil and roots. We present an in vivo analysis of local water uptake and transport by roots of soil-grown maize plants—for the first time measured in a three-dimensional time-resolved manner. Using deuterated water as tracer in infiltration experiments, we visualized soil imbibition, local root uptake, and tracked the transport of deuterated water throughout the fibrous root system for a day and night situation. This revealed significant differences in water transport between different root types. The primary root was the preferred water transport path in the 13-days-old plants while seminal roots of comparable size and length contributed little to plant water supply. The results underline the unique potential of fast neutron tomography to provide time-resolved 3D in vivo information on the water uptake and transport dynamics of plant root systems, thus contributing to a better understanding of the complex interactions of plant, soil and water. Nature Publishing Group UK 2021-05-19 /pmc/articles/PMC8134433/ /pubmed/34012044 http://dx.doi.org/10.1038/s41598-021-90062-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Tötzke, Christian
Kardjilov, Nikolay
Hilger, André
Rudolph-Mohr, Nicole
Manke, Ingo
Oswald, Sascha E.
Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_full Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_fullStr Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_full_unstemmed Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_short Three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
title_sort three-dimensional in vivo analysis of water uptake and translocation in maize roots by fast neutron tomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134433/
https://www.ncbi.nlm.nih.gov/pubmed/34012044
http://dx.doi.org/10.1038/s41598-021-90062-4
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