Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes

Preparation of long single-stranded (ss)DNA in large quantities with high efficiency and purity remains a synthetic challenge. Here, we present a protocol for using DNA-hydrolyzing DNA enzymes (deoxyribozymes) for efficient biotechnological production of milligrams of ssDNA with a customizable seque...

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Detalles Bibliográficos
Autores principales: Liu, Jin, Gu, Hongzhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134756/
https://www.ncbi.nlm.nih.gov/pubmed/34027488
http://dx.doi.org/10.1016/j.xpro.2021.100531
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author Liu, Jin
Gu, Hongzhou
author_facet Liu, Jin
Gu, Hongzhou
author_sort Liu, Jin
collection PubMed
description Preparation of long single-stranded (ss)DNA in large quantities with high efficiency and purity remains a synthetic challenge. Here, we present a protocol for using DNA-hydrolyzing DNA enzymes (deoxyribozymes) for efficient biotechnological production of milligrams of ssDNA with a customizable sequence up to a few kilobases. Our protocol provides a convenient yet economical way to store the sequence information of target ssDNA on phages for selective mass production on demand. For complete details on the use and execution of this protocol, please refer to Jia et al. (2021).
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spelling pubmed-81347562021-05-21 Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes Liu, Jin Gu, Hongzhou STAR Protoc Protocol Preparation of long single-stranded (ss)DNA in large quantities with high efficiency and purity remains a synthetic challenge. Here, we present a protocol for using DNA-hydrolyzing DNA enzymes (deoxyribozymes) for efficient biotechnological production of milligrams of ssDNA with a customizable sequence up to a few kilobases. Our protocol provides a convenient yet economical way to store the sequence information of target ssDNA on phages for selective mass production on demand. For complete details on the use and execution of this protocol, please refer to Jia et al. (2021). Elsevier 2021-05-10 /pmc/articles/PMC8134756/ /pubmed/34027488 http://dx.doi.org/10.1016/j.xpro.2021.100531 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Jin
Gu, Hongzhou
Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes
title Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes
title_full Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes
title_fullStr Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes
title_full_unstemmed Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes
title_short Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes
title_sort biotechnological production of ssdna with dna-hydrolyzing deoxyribozymes
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8134756/
https://www.ncbi.nlm.nih.gov/pubmed/34027488
http://dx.doi.org/10.1016/j.xpro.2021.100531
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