Polygonatum sibiricum F. Delaroche polysaccharide ameliorates HFD-induced mouse obesity via regulation of lipid metabolism and inflammatory response

The present study sought to elucidate the role of Polygonatum sibiricum F. Delaroche polysaccharide (PSP) in high-fat diet (HFD)-induced mouse obesity and investigated the primary molecular mechanism underlaying these effects. An obese mouse model was established by feeding HFD and three doses of PSP were administered intragastrically. Changes in body weight, serum lipids and parameters were recorded and the mechanism was explored by reverse transcription-quantitative PCR and western blotting. Body weight, blood lipids, blood glucose, insulin, resistin, adiponectin, liver weight and abdominal fat pads weight were reduced by PSP and abnormal expression levels of inflammatory factors such as TNF-α, IL-6, IL-1β and iNOS and lipid metabolism genes such as FAS, SREBP-1, PPARα and CPT-1were also reversed by PSP. The 5′ adenosine monophosphate-activated protein kinase (AMPK) signaling pathway was activated in PSP mouse liver, leading to lipid-lowering and anti-inflammatory effects. The results therefore suggested that PSP exhibited lipid-lowering and anti-inflammatory effects by activating the AMPK signaling pathway.