Effect of naturally isolated hydroquinone in disturbing the cell membrane integrity of Pseudomonas aeruginosa MTCC 741 and Staphylococcus aureus MTCC 740

Nosocomial pathogens cause various health problems in human and many novel drugs are under investigation to combat the pathogens. The present study explains the naturally derived hydroquinone possible mode of action against Pseudomonas aeruginosa MTCC 741 and Staphylococcus aureus MTCC 740. Time kill studies, cell viability assays, membrane potential assays, and potassium release assays were carried out to study the mode of action. Time kill studies revealed the rapid death of bacterial pathogens exposed to 4X MIC (Minimum inhibitory concentration) of the hydroquinone. Cell viability assay results showed that nearly half of the cell destruction of test pathogens occurred within one hour. Transmission electron microscopic (TEM) observations revealed the disruption of the cell membrane, which caused severe ultrastructural changes in both test pathogens. Hydroquinone dissipated the membrane potential of test pathogens, as confirmed by the depolarization of membrane potential, increases in permeability and leakage of intracellular potassium ions. At the concentration of 2X MIC hydroquinone in 5 min, about 91.41% and 84.85% potassium ions were released from P. aeruginosa MTCC 741 and S. aureus MTCC 740, respectively. This is the first report on the mode of action of naturally derived hydroquinone against clinical pathogens.