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The immune protection induced by a serine protease from the Trichinella spiralis adult against Trichinella spiralis infection in pigs
Trichinellosis is a major foodborne parasitosis caused by Trichinella spiralis. In the present study, a serine protease gene from an adult T. spiralis (Ts-Adsp) cDNA library was cloned, expressed in Escherichia coli and purified by Ni-affinity chromatography. Previous studies of our laboratory have...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136858/ https://www.ncbi.nlm.nih.gov/pubmed/33970910 http://dx.doi.org/10.1371/journal.pntd.0009408 |
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author | Xu, Daoxiu Bai, Xue Xu, Jing Wang, Xuelin Dong, Zijian Shi, Wenjie Xu, Fengyan Li, Yanfeng Liu, Mingyuan Liu, Xiaolei |
author_facet | Xu, Daoxiu Bai, Xue Xu, Jing Wang, Xuelin Dong, Zijian Shi, Wenjie Xu, Fengyan Li, Yanfeng Liu, Mingyuan Liu, Xiaolei |
author_sort | Xu, Daoxiu |
collection | PubMed |
description | Trichinellosis is a major foodborne parasitosis caused by Trichinella spiralis. In the present study, a serine protease gene from an adult T. spiralis (Ts-Adsp) cDNA library was cloned, expressed in Escherichia coli and purified by Ni-affinity chromatography. Previous studies of our laboratory have found that mice vaccinated with recombinant Ts-Adsp protein (rTs-Adsp) exhibited partial protection against T. spiralis infection. In this study, the protective effect of rTs-Adsp against T. spiralis infection in pigs was further explored. The cell-mediated and humoral immune responses induced by rTs-Adsp were measured, including the dynamic trends of specific antibody levels (IgG, IgG1, IgG2a and IgM), as well as the levels of cytokines (IFN-γ, IL-2, IL-4, and IL-10) in the serum. Moreover, the changes in T lymphocytes, B lymphocytes, and neutrophils were measured to evaluate cellular immune responses in pigs vaccinated with rTs-Adsp. The results indicated that a Th1-Th2 mixed immune response with Th1 predominant was induced by rTs-Adsp after vaccination. Flow cytometric analysis showed that the proportions of CD4(+) T cells, B cells, and neutrophils in the immunized groups were significantly increased. Furthermore, pigs vaccinated with rTs-Adsp exhibited a 50.9% reduction in the muscle larvae burden, compare with pigs from the PBS group five weeks after challenged. Our results suggested that rTs-Adsp elicited partial protection and it could be a potential target molecule for preventing and controlling Trichinella transmission from pigs to human. |
format | Online Article Text |
id | pubmed-8136858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81368582021-06-02 The immune protection induced by a serine protease from the Trichinella spiralis adult against Trichinella spiralis infection in pigs Xu, Daoxiu Bai, Xue Xu, Jing Wang, Xuelin Dong, Zijian Shi, Wenjie Xu, Fengyan Li, Yanfeng Liu, Mingyuan Liu, Xiaolei PLoS Negl Trop Dis Research Article Trichinellosis is a major foodborne parasitosis caused by Trichinella spiralis. In the present study, a serine protease gene from an adult T. spiralis (Ts-Adsp) cDNA library was cloned, expressed in Escherichia coli and purified by Ni-affinity chromatography. Previous studies of our laboratory have found that mice vaccinated with recombinant Ts-Adsp protein (rTs-Adsp) exhibited partial protection against T. spiralis infection. In this study, the protective effect of rTs-Adsp against T. spiralis infection in pigs was further explored. The cell-mediated and humoral immune responses induced by rTs-Adsp were measured, including the dynamic trends of specific antibody levels (IgG, IgG1, IgG2a and IgM), as well as the levels of cytokines (IFN-γ, IL-2, IL-4, and IL-10) in the serum. Moreover, the changes in T lymphocytes, B lymphocytes, and neutrophils were measured to evaluate cellular immune responses in pigs vaccinated with rTs-Adsp. The results indicated that a Th1-Th2 mixed immune response with Th1 predominant was induced by rTs-Adsp after vaccination. Flow cytometric analysis showed that the proportions of CD4(+) T cells, B cells, and neutrophils in the immunized groups were significantly increased. Furthermore, pigs vaccinated with rTs-Adsp exhibited a 50.9% reduction in the muscle larvae burden, compare with pigs from the PBS group five weeks after challenged. Our results suggested that rTs-Adsp elicited partial protection and it could be a potential target molecule for preventing and controlling Trichinella transmission from pigs to human. Public Library of Science 2021-05-10 /pmc/articles/PMC8136858/ /pubmed/33970910 http://dx.doi.org/10.1371/journal.pntd.0009408 Text en © 2021 Xu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Xu, Daoxiu Bai, Xue Xu, Jing Wang, Xuelin Dong, Zijian Shi, Wenjie Xu, Fengyan Li, Yanfeng Liu, Mingyuan Liu, Xiaolei The immune protection induced by a serine protease from the Trichinella spiralis adult against Trichinella spiralis infection in pigs |
title | The immune protection induced by a serine protease from the Trichinella spiralis adult against Trichinella spiralis infection in pigs |
title_full | The immune protection induced by a serine protease from the Trichinella spiralis adult against Trichinella spiralis infection in pigs |
title_fullStr | The immune protection induced by a serine protease from the Trichinella spiralis adult against Trichinella spiralis infection in pigs |
title_full_unstemmed | The immune protection induced by a serine protease from the Trichinella spiralis adult against Trichinella spiralis infection in pigs |
title_short | The immune protection induced by a serine protease from the Trichinella spiralis adult against Trichinella spiralis infection in pigs |
title_sort | immune protection induced by a serine protease from the trichinella spiralis adult against trichinella spiralis infection in pigs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136858/ https://www.ncbi.nlm.nih.gov/pubmed/33970910 http://dx.doi.org/10.1371/journal.pntd.0009408 |
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