Cargando…

The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality

In wheat (Triticum aestivum L.), breeding efforts have focused intensively on improving grain yield and quality. For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality. Moreover, starch levels in seeds are associa...

Descripción completa

Detalles Bibliográficos
Autores principales: Gao, Yujiao, An, Kexin, Guo, Weiwei, Chen, Yongming, Zhang, Ruijie, Zhang, Xue, Chang, Siyuan, Rossi, Vincenzo, Jin, Fangming, Cao, Xinyou, Xin, Mingming, Peng, Huiru, Hu, Zhaorong, Guo, Weilong, Du, Jinkun, Ni, Zhongfu, Sun, Qixin, Yao, Yingyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136912/
https://www.ncbi.nlm.nih.gov/pubmed/33955492
http://dx.doi.org/10.1093/plcell/koaa040
_version_ 1783695518818369536
author Gao, Yujiao
An, Kexin
Guo, Weiwei
Chen, Yongming
Zhang, Ruijie
Zhang, Xue
Chang, Siyuan
Rossi, Vincenzo
Jin, Fangming
Cao, Xinyou
Xin, Mingming
Peng, Huiru
Hu, Zhaorong
Guo, Weilong
Du, Jinkun
Ni, Zhongfu
Sun, Qixin
Yao, Yingyin
author_facet Gao, Yujiao
An, Kexin
Guo, Weiwei
Chen, Yongming
Zhang, Ruijie
Zhang, Xue
Chang, Siyuan
Rossi, Vincenzo
Jin, Fangming
Cao, Xinyou
Xin, Mingming
Peng, Huiru
Hu, Zhaorong
Guo, Weilong
Du, Jinkun
Ni, Zhongfu
Sun, Qixin
Yao, Yingyin
author_sort Gao, Yujiao
collection PubMed
description In wheat (Triticum aestivum L.), breeding efforts have focused intensively on improving grain yield and quality. For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality. Moreover, starch levels in seeds are associated with yield. However, little is known about the mechanisms that coordinate SSP and starch accumulation in wheat. In this study, we explored the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation. TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes. Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters. TaNAC019 directly activated the expression of HMW-GS genes by binding to a specific motif in their promoters and interacting with the TaGlu-1 regulator TaGAMyb. TaNAC019 also indirectly regulated the expression of TaSPA, an ortholog of maize Opaque2 that activates SSP accumulation. Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality. Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality.
format Online
Article
Text
id pubmed-8136912
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-81369122021-05-26 The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality Gao, Yujiao An, Kexin Guo, Weiwei Chen, Yongming Zhang, Ruijie Zhang, Xue Chang, Siyuan Rossi, Vincenzo Jin, Fangming Cao, Xinyou Xin, Mingming Peng, Huiru Hu, Zhaorong Guo, Weilong Du, Jinkun Ni, Zhongfu Sun, Qixin Yao, Yingyin Plant Cell Research Articles In wheat (Triticum aestivum L.), breeding efforts have focused intensively on improving grain yield and quality. For quality, the content and composition of seed storage proteins (SSPs) determine the elasticity of wheat dough and flour processing quality. Moreover, starch levels in seeds are associated with yield. However, little is known about the mechanisms that coordinate SSP and starch accumulation in wheat. In this study, we explored the role of the endosperm-specific NAC transcription factor TaNAC019 in coordinating SSP and starch accumulation. TaNAC019 binds to the promoters of TaGlu-1 loci, encoding high molecular weight glutenin (HMW-GS), and of starch metabolism genes. Triple knock-out mutants of all three TaNAC019 homoeologs exhibited reduced transcript levels for all SSP types and genes involved in starch metabolism, leading to lower gluten and starch contents, and in flour processing quality parameters. TaNAC019 directly activated the expression of HMW-GS genes by binding to a specific motif in their promoters and interacting with the TaGlu-1 regulator TaGAMyb. TaNAC019 also indirectly regulated the expression of TaSPA, an ortholog of maize Opaque2 that activates SSP accumulation. Therefore, TaNAC019 regulation of starch- and SSP-related genes has key roles in wheat grain quality. Finally, we identified an elite allele (TaNAC019-BI) associated with flour processing quality, providing a candidate gene for breeding wheat with improved quality. Oxford University Press 2021-01-02 /pmc/articles/PMC8136912/ /pubmed/33955492 http://dx.doi.org/10.1093/plcell/koaa040 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Research Articles
Gao, Yujiao
An, Kexin
Guo, Weiwei
Chen, Yongming
Zhang, Ruijie
Zhang, Xue
Chang, Siyuan
Rossi, Vincenzo
Jin, Fangming
Cao, Xinyou
Xin, Mingming
Peng, Huiru
Hu, Zhaorong
Guo, Weilong
Du, Jinkun
Ni, Zhongfu
Sun, Qixin
Yao, Yingyin
The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality
title The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality
title_full The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality
title_fullStr The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality
title_full_unstemmed The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality
title_short The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality
title_sort endosperm-specific transcription factor tanac019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8136912/
https://www.ncbi.nlm.nih.gov/pubmed/33955492
http://dx.doi.org/10.1093/plcell/koaa040
work_keys_str_mv AT gaoyujiao theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT ankexin theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT guoweiwei theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT chenyongming theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT zhangruijie theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT zhangxue theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT changsiyuan theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT rossivincenzo theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT jinfangming theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT caoxinyou theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT xinmingming theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT penghuiru theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT huzhaorong theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT guoweilong theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT dujinkun theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT nizhongfu theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT sunqixin theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT yaoyingyin theendospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT gaoyujiao endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT ankexin endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT guoweiwei endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT chenyongming endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT zhangruijie endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT zhangxue endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT changsiyuan endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT rossivincenzo endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT jinfangming endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT caoxinyou endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT xinmingming endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT penghuiru endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT huzhaorong endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT guoweilong endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT dujinkun endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT nizhongfu endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT sunqixin endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality
AT yaoyingyin endospermspecifictranscriptionfactortanac019regulatesgluteninandstarchaccumulationanditselitealleleimproveswheatgrainquality