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Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment

OBJECTIVES: The aim of this study was to investigate changes in saliva concentration of the inflammatory marker MRP-8/14 and the presence of some periodontitis-associated bacteria in patients with mixed dentition treated with a rigid acrylic, bonded maxillary expander. METHODS: Fifteen patients in m...

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Autores principales: Nemec, Michael, Mittinger, Nina, Bertl, Michael, Liu, Emanuela, Jonke, Erwin, Andrukhov, Oleh, Rausch-Fan, Xiaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137619/
https://www.ncbi.nlm.nih.gov/pubmed/33270150
http://dx.doi.org/10.1007/s00784-020-03706-6
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author Nemec, Michael
Mittinger, Nina
Bertl, Michael
Liu, Emanuela
Jonke, Erwin
Andrukhov, Oleh
Rausch-Fan, Xiaohui
author_facet Nemec, Michael
Mittinger, Nina
Bertl, Michael
Liu, Emanuela
Jonke, Erwin
Andrukhov, Oleh
Rausch-Fan, Xiaohui
author_sort Nemec, Michael
collection PubMed
description OBJECTIVES: The aim of this study was to investigate changes in saliva concentration of the inflammatory marker MRP-8/14 and the presence of some periodontitis-associated bacteria in patients with mixed dentition treated with a rigid acrylic, bonded maxillary expander. METHODS: Fifteen patients in mixed dentition treated with a bonded palatal expander were enrolled in this longitudinal study. Saliva samples were taken before the therapy, as well as in 2 weeks and 3, 6, 9, and 12 months after the beginning of the therapy. In each sample, the levels of MRP-8/14 were determined by ELISA and the presence of 11 bacteria was detected by PCR followed by DNA-DNA hybridization. RESULTS: Salivary concentration of MRP-8/14 and the amount of Tannerella forsythia, Treponema denticola, and Eikenella corrodens were significantly increased during treatment with bonded maxillary expander. These changes were transient and the maximal levels of MRP-8/14 and periodontitis-associated pathogens were observed 6–9 months after the beginning of the therapy. CONCLUSION: Therapy with bonded maxillary results in higher MRP-8/14 levels and increased prevalence of some periodontitis-associated bacteria, namely T. forsythia, T. denticola, and E. corrodens. The results suggest the detection of salivary MRP-8/14 levels may be a potential tool to reflect the oral health status in children with fixed orthodontic treatment. CLINICAL RELEVANCE: Our data suggest that the treatment with bonded maxillary expander might influence the oral health status and should be accompanied by the careful control of the oral health during the therapy.
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spelling pubmed-81376192021-06-03 Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment Nemec, Michael Mittinger, Nina Bertl, Michael Liu, Emanuela Jonke, Erwin Andrukhov, Oleh Rausch-Fan, Xiaohui Clin Oral Investig Original Article OBJECTIVES: The aim of this study was to investigate changes in saliva concentration of the inflammatory marker MRP-8/14 and the presence of some periodontitis-associated bacteria in patients with mixed dentition treated with a rigid acrylic, bonded maxillary expander. METHODS: Fifteen patients in mixed dentition treated with a bonded palatal expander were enrolled in this longitudinal study. Saliva samples were taken before the therapy, as well as in 2 weeks and 3, 6, 9, and 12 months after the beginning of the therapy. In each sample, the levels of MRP-8/14 were determined by ELISA and the presence of 11 bacteria was detected by PCR followed by DNA-DNA hybridization. RESULTS: Salivary concentration of MRP-8/14 and the amount of Tannerella forsythia, Treponema denticola, and Eikenella corrodens were significantly increased during treatment with bonded maxillary expander. These changes were transient and the maximal levels of MRP-8/14 and periodontitis-associated pathogens were observed 6–9 months after the beginning of the therapy. CONCLUSION: Therapy with bonded maxillary results in higher MRP-8/14 levels and increased prevalence of some periodontitis-associated bacteria, namely T. forsythia, T. denticola, and E. corrodens. The results suggest the detection of salivary MRP-8/14 levels may be a potential tool to reflect the oral health status in children with fixed orthodontic treatment. CLINICAL RELEVANCE: Our data suggest that the treatment with bonded maxillary expander might influence the oral health status and should be accompanied by the careful control of the oral health during the therapy. Springer Berlin Heidelberg 2020-12-03 2021 /pmc/articles/PMC8137619/ /pubmed/33270150 http://dx.doi.org/10.1007/s00784-020-03706-6 Text en © The Author(s) 2020 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Nemec, Michael
Mittinger, Nina
Bertl, Michael
Liu, Emanuela
Jonke, Erwin
Andrukhov, Oleh
Rausch-Fan, Xiaohui
Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment
title Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment
title_full Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment
title_fullStr Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment
title_full_unstemmed Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment
title_short Salivary MRP-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment
title_sort salivary mrp-8/14 and the presence of periodontitis-associated bacteria in children with bonded maxillary expansion treatment
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137619/
https://www.ncbi.nlm.nih.gov/pubmed/33270150
http://dx.doi.org/10.1007/s00784-020-03706-6
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