Cargando…
Whole transcriptome RNA-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of Asian Indians
The prevalence of Type 2 Diabetes has reached an epidemic proportion particularly in south Asian countries. We have earlier shown that the anatomical fat distribution, termed ‘thin fat phenotype’ in this population indeed plays a major role for their T2D-predisposition it is indeed the sick fat or a...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137704/ https://www.ncbi.nlm.nih.gov/pubmed/34017037 http://dx.doi.org/10.1038/s41598-021-90148-z |
_version_ | 1783695660023808000 |
---|---|
author | Saxena, Aditya Mathur, Nitish Tiwari, Pradeep Mathur, Sandeep Kumar |
author_facet | Saxena, Aditya Mathur, Nitish Tiwari, Pradeep Mathur, Sandeep Kumar |
author_sort | Saxena, Aditya |
collection | PubMed |
description | The prevalence of Type 2 Diabetes has reached an epidemic proportion particularly in south Asian countries. We have earlier shown that the anatomical fat distribution, termed ‘thin fat phenotype’ in this population indeed plays a major role for their T2D-predisposition it is indeed the sick fat or adiposopathy, which is the root cause of metabolic syndrome and diabetes and affects both—peripheral, as well as visceral adipose tissue compartments. In present study, we have attempted to unravel the altered regulatory mechanisms at the level of transcription factors, and miRNAs those may likely accounts to T2D pathophysiology in femoral subcutaneous adipose tissue. We prioritized transcription factors and protein kinases as likely upstream regulators of obtained differentially expressed genes in this RNA-seq study. An inferred network of these upstream regulators was then derived and the role of TFs and miRNAs in T2D pathophysiology was explored. In conclusions, this RNS-Seq study finds that peripheral subcutaneous adipose tissue among Asian Indians show pathology characterized by altered lipid, glucose and protein metabolism, adipogenesis defect and inflammation. A network of regulatory transcription factors, protein kinases and microRNAs have been imputed which converge on the process of adipogenesis. As the majority of these genes also showed altered expression in diabetics and some of them are also circulatory, therefore they deserve further investigation for potential clinical diagnostic and therapeutic applications. |
format | Online Article Text |
id | pubmed-8137704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81377042021-05-25 Whole transcriptome RNA-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of Asian Indians Saxena, Aditya Mathur, Nitish Tiwari, Pradeep Mathur, Sandeep Kumar Sci Rep Article The prevalence of Type 2 Diabetes has reached an epidemic proportion particularly in south Asian countries. We have earlier shown that the anatomical fat distribution, termed ‘thin fat phenotype’ in this population indeed plays a major role for their T2D-predisposition it is indeed the sick fat or adiposopathy, which is the root cause of metabolic syndrome and diabetes and affects both—peripheral, as well as visceral adipose tissue compartments. In present study, we have attempted to unravel the altered regulatory mechanisms at the level of transcription factors, and miRNAs those may likely accounts to T2D pathophysiology in femoral subcutaneous adipose tissue. We prioritized transcription factors and protein kinases as likely upstream regulators of obtained differentially expressed genes in this RNA-seq study. An inferred network of these upstream regulators was then derived and the role of TFs and miRNAs in T2D pathophysiology was explored. In conclusions, this RNS-Seq study finds that peripheral subcutaneous adipose tissue among Asian Indians show pathology characterized by altered lipid, glucose and protein metabolism, adipogenesis defect and inflammation. A network of regulatory transcription factors, protein kinases and microRNAs have been imputed which converge on the process of adipogenesis. As the majority of these genes also showed altered expression in diabetics and some of them are also circulatory, therefore they deserve further investigation for potential clinical diagnostic and therapeutic applications. Nature Publishing Group UK 2021-05-20 /pmc/articles/PMC8137704/ /pubmed/34017037 http://dx.doi.org/10.1038/s41598-021-90148-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Saxena, Aditya Mathur, Nitish Tiwari, Pradeep Mathur, Sandeep Kumar Whole transcriptome RNA-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of Asian Indians |
title | Whole transcriptome RNA-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of Asian Indians |
title_full | Whole transcriptome RNA-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of Asian Indians |
title_fullStr | Whole transcriptome RNA-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of Asian Indians |
title_full_unstemmed | Whole transcriptome RNA-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of Asian Indians |
title_short | Whole transcriptome RNA-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of Asian Indians |
title_sort | whole transcriptome rna-seq reveals key regulatory factors involved in type 2 diabetes pathology in peripheral fat of asian indians |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137704/ https://www.ncbi.nlm.nih.gov/pubmed/34017037 http://dx.doi.org/10.1038/s41598-021-90148-z |
work_keys_str_mv | AT saxenaaditya wholetranscriptomernaseqrevealskeyregulatoryfactorsinvolvedintype2diabetespathologyinperipheralfatofasianindians AT mathurnitish wholetranscriptomernaseqrevealskeyregulatoryfactorsinvolvedintype2diabetespathologyinperipheralfatofasianindians AT tiwaripradeep wholetranscriptomernaseqrevealskeyregulatoryfactorsinvolvedintype2diabetespathologyinperipheralfatofasianindians AT mathursandeepkumar wholetranscriptomernaseqrevealskeyregulatoryfactorsinvolvedintype2diabetespathologyinperipheralfatofasianindians |