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Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination
Two-photon excitation can lower phototoxicity and improve penetration depth, but its narrow excitation range restricts its applications in light-sheet microscopy. Here, we propose simple illumination optics, a lens-axicon triplet composed of an axicon and two convex lenses, to generate longer extent...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8137944/ https://www.ncbi.nlm.nih.gov/pubmed/34016994 http://dx.doi.org/10.1038/s41467-021-23249-y |