Cargando…

Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum

Soil-occupant fungi produce a variety of mycotoxins as secondary metabolites, one of which is mycophenolic acid (MPA), an antibiotic and immunosuppressive agent. MPA is mainly produced by several species of Penicillium, especially Penicillium brevicompactum. Here, we present the first report of MPA...

Descripción completa

Detalles Bibliográficos
Autores principales: Mahmoudian, Fatemeh, sharifirad, Atefeh, Yakhchali, Bagher, Ansari, Saham, Fatemi, Seyed Safa-ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138112/
https://www.ncbi.nlm.nih.gov/pubmed/34019120
http://dx.doi.org/10.1007/s00284-021-02509-6
_version_ 1783695745022427136
author Mahmoudian, Fatemeh
sharifirad, Atefeh
Yakhchali, Bagher
Ansari, Saham
Fatemi, Seyed Safa-ali
author_facet Mahmoudian, Fatemeh
sharifirad, Atefeh
Yakhchali, Bagher
Ansari, Saham
Fatemi, Seyed Safa-ali
author_sort Mahmoudian, Fatemeh
collection PubMed
description Soil-occupant fungi produce a variety of mycotoxins as secondary metabolites, one of which is mycophenolic acid (MPA), an antibiotic and immunosuppressive agent. MPA is mainly produced by several species of Penicillium, especially Penicillium brevicompactum. Here, we present the first report of MPA production by a local strain belonging to Penicillium glabrum species. We screened ascomycete cultures isolated from moldy food and fruits, as well as soils, collected from different parts of Iran. MPA production of one hundred and forty Penicillium isolates was analyzed using HPLC. Three MPA producer isolates were identified, among which the most producer was subjected to further characterization, based on morphological and microscopic analysis, as well as molecular approach (ITS, rDNA and beta-tubulin gene sequences). The results revealed that the best MPA producer belongs to P. glabrum IBRC-M 30518, and can produce 1079 mg/L MPA in Czapek-Dox medium.
format Online
Article
Text
id pubmed-8138112
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-81381122021-05-21 Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum Mahmoudian, Fatemeh sharifirad, Atefeh Yakhchali, Bagher Ansari, Saham Fatemi, Seyed Safa-ali Curr Microbiol Article Soil-occupant fungi produce a variety of mycotoxins as secondary metabolites, one of which is mycophenolic acid (MPA), an antibiotic and immunosuppressive agent. MPA is mainly produced by several species of Penicillium, especially Penicillium brevicompactum. Here, we present the first report of MPA production by a local strain belonging to Penicillium glabrum species. We screened ascomycete cultures isolated from moldy food and fruits, as well as soils, collected from different parts of Iran. MPA production of one hundred and forty Penicillium isolates was analyzed using HPLC. Three MPA producer isolates were identified, among which the most producer was subjected to further characterization, based on morphological and microscopic analysis, as well as molecular approach (ITS, rDNA and beta-tubulin gene sequences). The results revealed that the best MPA producer belongs to P. glabrum IBRC-M 30518, and can produce 1079 mg/L MPA in Czapek-Dox medium. Springer US 2021-05-21 2021 /pmc/articles/PMC8138112/ /pubmed/34019120 http://dx.doi.org/10.1007/s00284-021-02509-6 Text en © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Mahmoudian, Fatemeh
sharifirad, Atefeh
Yakhchali, Bagher
Ansari, Saham
Fatemi, Seyed Safa-ali
Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum
title Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum
title_full Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum
title_fullStr Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum
title_full_unstemmed Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum
title_short Production of Mycophenolic Acid by a Newly Isolated Indigenous Penicillium glabrum
title_sort production of mycophenolic acid by a newly isolated indigenous penicillium glabrum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138112/
https://www.ncbi.nlm.nih.gov/pubmed/34019120
http://dx.doi.org/10.1007/s00284-021-02509-6
work_keys_str_mv AT mahmoudianfatemeh productionofmycophenolicacidbyanewlyisolatedindigenouspenicilliumglabrum
AT sharifiradatefeh productionofmycophenolicacidbyanewlyisolatedindigenouspenicilliumglabrum
AT yakhchalibagher productionofmycophenolicacidbyanewlyisolatedindigenouspenicilliumglabrum
AT ansarisaham productionofmycophenolicacidbyanewlyisolatedindigenouspenicilliumglabrum
AT fatemiseyedsafaali productionofmycophenolicacidbyanewlyisolatedindigenouspenicilliumglabrum