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Heterogeneity of Pulmonary Granulomas in Cattle Experimentally Infected With Mycobacterium bovis

Mycobacterium bovis is the cause of tuberculosis in most animals, most notably cattle. The stereotypical lesion of bovine tuberculosis is the granuloma; a distinct morphological lesion where host and pathogen interact and disease outcome (i.e., dissemination, confinement, or resolution) is determine...

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Autores principales: Palmer, Mitchell V., Thacker, Tyler C., Kanipe, Carly, Boggiatto, Paola M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138452/
https://www.ncbi.nlm.nih.gov/pubmed/34026898
http://dx.doi.org/10.3389/fvets.2021.671460
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author Palmer, Mitchell V.
Thacker, Tyler C.
Kanipe, Carly
Boggiatto, Paola M.
author_facet Palmer, Mitchell V.
Thacker, Tyler C.
Kanipe, Carly
Boggiatto, Paola M.
author_sort Palmer, Mitchell V.
collection PubMed
description Mycobacterium bovis is the cause of tuberculosis in most animals, most notably cattle. The stereotypical lesion of bovine tuberculosis is the granuloma; a distinct morphological lesion where host and pathogen interact and disease outcome (i.e., dissemination, confinement, or resolution) is determined. Accordingly, it is critical to understand host-pathogen interactions at the granuloma level. Host-pathogen interactions within individual granulomas at different stages of disease have not been examined in cattle. We examined bacterial burden and cytokine expression in individual pulmonary granulomas from steers at 30, 90, 180, and 270 days after experimental aerosol infection with M. bovis. Bacterial burdens within individual granulomas examined 30 days after infection were greater and more heterogenous (variable) than those examined 90 to 270 days after infection. Bacterial burdens did not correlate with expression of IFN-γ, TNF-α, TGF-β, granuloma stage, or lung lesion score, although there was a modest positive correlation with IL-10 expression. Granuloma stage did have modest positive and negative correlations with TNF-α and IL-10, respectively. Heterogeneity and mean expression of IFN-γ, IL-10 and TNF-α did not differ significantly over time, however, expression of TGF-β at 90 days was significantly greater than that seen at 30 days after infection.
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spelling pubmed-81384522021-05-22 Heterogeneity of Pulmonary Granulomas in Cattle Experimentally Infected With Mycobacterium bovis Palmer, Mitchell V. Thacker, Tyler C. Kanipe, Carly Boggiatto, Paola M. Front Vet Sci Veterinary Science Mycobacterium bovis is the cause of tuberculosis in most animals, most notably cattle. The stereotypical lesion of bovine tuberculosis is the granuloma; a distinct morphological lesion where host and pathogen interact and disease outcome (i.e., dissemination, confinement, or resolution) is determined. Accordingly, it is critical to understand host-pathogen interactions at the granuloma level. Host-pathogen interactions within individual granulomas at different stages of disease have not been examined in cattle. We examined bacterial burden and cytokine expression in individual pulmonary granulomas from steers at 30, 90, 180, and 270 days after experimental aerosol infection with M. bovis. Bacterial burdens within individual granulomas examined 30 days after infection were greater and more heterogenous (variable) than those examined 90 to 270 days after infection. Bacterial burdens did not correlate with expression of IFN-γ, TNF-α, TGF-β, granuloma stage, or lung lesion score, although there was a modest positive correlation with IL-10 expression. Granuloma stage did have modest positive and negative correlations with TNF-α and IL-10, respectively. Heterogeneity and mean expression of IFN-γ, IL-10 and TNF-α did not differ significantly over time, however, expression of TGF-β at 90 days was significantly greater than that seen at 30 days after infection. Frontiers Media S.A. 2021-05-07 /pmc/articles/PMC8138452/ /pubmed/34026898 http://dx.doi.org/10.3389/fvets.2021.671460 Text en Copyright © 2021 Palmer, Thacker, Kanipe and Boggiatto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Palmer, Mitchell V.
Thacker, Tyler C.
Kanipe, Carly
Boggiatto, Paola M.
Heterogeneity of Pulmonary Granulomas in Cattle Experimentally Infected With Mycobacterium bovis
title Heterogeneity of Pulmonary Granulomas in Cattle Experimentally Infected With Mycobacterium bovis
title_full Heterogeneity of Pulmonary Granulomas in Cattle Experimentally Infected With Mycobacterium bovis
title_fullStr Heterogeneity of Pulmonary Granulomas in Cattle Experimentally Infected With Mycobacterium bovis
title_full_unstemmed Heterogeneity of Pulmonary Granulomas in Cattle Experimentally Infected With Mycobacterium bovis
title_short Heterogeneity of Pulmonary Granulomas in Cattle Experimentally Infected With Mycobacterium bovis
title_sort heterogeneity of pulmonary granulomas in cattle experimentally infected with mycobacterium bovis
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138452/
https://www.ncbi.nlm.nih.gov/pubmed/34026898
http://dx.doi.org/10.3389/fvets.2021.671460
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