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Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles

Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and path...

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Autores principales: Erdbrügger, Uta, Blijdorp, Charles J., Bijnsdorp, Irene V., Borràs, Francesc E., Burger, Dylan, Bussolati, Benedetta, Byrd, James Brian, Clayton, Aled, Dear, James W., Falcón‐Pérez, Juan M., Grange, Cristina, Hill, Andrew F., Holthöfer, Harry, Hoorn, Ewout J., Jenster, Guido, Jimenez, Connie R., Junker, Kerstin, Klein, John, Knepper, Mark A., Koritzinsky, Erik H., Luther, James M., Lenassi, Metka, Leivo, Janne, Mertens, Inge, Musante, Luca, Oeyen, Eline, Puhka, Maija, van Royen, Martin E., Sánchez, Catherine, Soekmadji, Carolina, Thongboonkerd, Visith, van Steijn, Volkert, Verhaegh, Gerald, Webber, Jason P., Witwer, Kenneth, Yuen, Peter S.T., Zheng, Lei, Llorente, Alicia, Martens‐Uzunova, Elena S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138533/
https://www.ncbi.nlm.nih.gov/pubmed/34035881
http://dx.doi.org/10.1002/jev2.12093
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author Erdbrügger, Uta
Blijdorp, Charles J.
Bijnsdorp, Irene V.
Borràs, Francesc E.
Burger, Dylan
Bussolati, Benedetta
Byrd, James Brian
Clayton, Aled
Dear, James W.
Falcón‐Pérez, Juan M.
Grange, Cristina
Hill, Andrew F.
Holthöfer, Harry
Hoorn, Ewout J.
Jenster, Guido
Jimenez, Connie R.
Junker, Kerstin
Klein, John
Knepper, Mark A.
Koritzinsky, Erik H.
Luther, James M.
Lenassi, Metka
Leivo, Janne
Mertens, Inge
Musante, Luca
Oeyen, Eline
Puhka, Maija
van Royen, Martin E.
Sánchez, Catherine
Soekmadji, Carolina
Thongboonkerd, Visith
van Steijn, Volkert
Verhaegh, Gerald
Webber, Jason P.
Witwer, Kenneth
Yuen, Peter S.T.
Zheng, Lei
Llorente, Alicia
Martens‐Uzunova, Elena S.
author_facet Erdbrügger, Uta
Blijdorp, Charles J.
Bijnsdorp, Irene V.
Borràs, Francesc E.
Burger, Dylan
Bussolati, Benedetta
Byrd, James Brian
Clayton, Aled
Dear, James W.
Falcón‐Pérez, Juan M.
Grange, Cristina
Hill, Andrew F.
Holthöfer, Harry
Hoorn, Ewout J.
Jenster, Guido
Jimenez, Connie R.
Junker, Kerstin
Klein, John
Knepper, Mark A.
Koritzinsky, Erik H.
Luther, James M.
Lenassi, Metka
Leivo, Janne
Mertens, Inge
Musante, Luca
Oeyen, Eline
Puhka, Maija
van Royen, Martin E.
Sánchez, Catherine
Soekmadji, Carolina
Thongboonkerd, Visith
van Steijn, Volkert
Verhaegh, Gerald
Webber, Jason P.
Witwer, Kenneth
Yuen, Peter S.T.
Zheng, Lei
Llorente, Alicia
Martens‐Uzunova, Elena S.
author_sort Erdbrügger, Uta
collection PubMed
description Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV‐based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
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spelling pubmed-81385332021-05-24 Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles Erdbrügger, Uta Blijdorp, Charles J. Bijnsdorp, Irene V. Borràs, Francesc E. Burger, Dylan Bussolati, Benedetta Byrd, James Brian Clayton, Aled Dear, James W. Falcón‐Pérez, Juan M. Grange, Cristina Hill, Andrew F. Holthöfer, Harry Hoorn, Ewout J. Jenster, Guido Jimenez, Connie R. Junker, Kerstin Klein, John Knepper, Mark A. Koritzinsky, Erik H. Luther, James M. Lenassi, Metka Leivo, Janne Mertens, Inge Musante, Luca Oeyen, Eline Puhka, Maija van Royen, Martin E. Sánchez, Catherine Soekmadji, Carolina Thongboonkerd, Visith van Steijn, Volkert Verhaegh, Gerald Webber, Jason P. Witwer, Kenneth Yuen, Peter S.T. Zheng, Lei Llorente, Alicia Martens‐Uzunova, Elena S. J Extracell Vesicles Position Papers Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV‐based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field. John Wiley and Sons Inc. 2021-05-21 2021-05 /pmc/articles/PMC8138533/ /pubmed/34035881 http://dx.doi.org/10.1002/jev2.12093 Text en © 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Position Papers
Erdbrügger, Uta
Blijdorp, Charles J.
Bijnsdorp, Irene V.
Borràs, Francesc E.
Burger, Dylan
Bussolati, Benedetta
Byrd, James Brian
Clayton, Aled
Dear, James W.
Falcón‐Pérez, Juan M.
Grange, Cristina
Hill, Andrew F.
Holthöfer, Harry
Hoorn, Ewout J.
Jenster, Guido
Jimenez, Connie R.
Junker, Kerstin
Klein, John
Knepper, Mark A.
Koritzinsky, Erik H.
Luther, James M.
Lenassi, Metka
Leivo, Janne
Mertens, Inge
Musante, Luca
Oeyen, Eline
Puhka, Maija
van Royen, Martin E.
Sánchez, Catherine
Soekmadji, Carolina
Thongboonkerd, Visith
van Steijn, Volkert
Verhaegh, Gerald
Webber, Jason P.
Witwer, Kenneth
Yuen, Peter S.T.
Zheng, Lei
Llorente, Alicia
Martens‐Uzunova, Elena S.
Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title_full Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title_fullStr Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title_full_unstemmed Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title_short Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title_sort urinary extracellular vesicles: a position paper by the urine task force of the international society for extracellular vesicles
topic Position Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138533/
https://www.ncbi.nlm.nih.gov/pubmed/34035881
http://dx.doi.org/10.1002/jev2.12093
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