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Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers

It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices. Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes. Our recent report on lithium hexamethyldisilazide...

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Autores principales: Lu, Ziyi, Wu, Yueming, Cong, Zihao, Qian, Yuxin, Wu, Xue, Shao, Ning, Qiao, Zhongqian, Zhang, Haodong, She, Yunrui, Chen, Kang, Xiang, Hengxue, Sun, Bin, Yu, Qian, Yuan, Yuan, Lin, Haodong, Zhu, Meifang, Liu, Runhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138731/
https://www.ncbi.nlm.nih.gov/pubmed/34027238
http://dx.doi.org/10.1016/j.bioactmat.2021.05.008
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author Lu, Ziyi
Wu, Yueming
Cong, Zihao
Qian, Yuxin
Wu, Xue
Shao, Ning
Qiao, Zhongqian
Zhang, Haodong
She, Yunrui
Chen, Kang
Xiang, Hengxue
Sun, Bin
Yu, Qian
Yuan, Yuan
Lin, Haodong
Zhu, Meifang
Liu, Runhui
author_facet Lu, Ziyi
Wu, Yueming
Cong, Zihao
Qian, Yuxin
Wu, Xue
Shao, Ning
Qiao, Zhongqian
Zhang, Haodong
She, Yunrui
Chen, Kang
Xiang, Hengxue
Sun, Bin
Yu, Qian
Yuan, Yuan
Lin, Haodong
Zhu, Meifang
Liu, Runhui
author_sort Lu, Ziyi
collection PubMed
description It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices. Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes. Our recent report on lithium hexamethyldisilazide-initiated superfast polymerization on amino acid N-carboxyanhydrides enables the quick synthesis of host defense peptide-mimicking peptide polymers. Here we reported a facile and cost-effective thermoplastic polyurethane (TPU) surface modification of peptide polymer (DLL: BLG = 90 : 10) using plasma surface activation and substitution reaction between thiol and bromide groups. The peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in vitro. Furthermore, the peptide polymer-modified TPU surfaces showed excellent biocompatibility, displaying no hemolysis and cytotoxicity. In vivo study using methicillin-resistant Staphylococcus aureus (MRSA) for subcutaneous implantation infectious model showed that peptide polymer-modified TPU surfaces revealed obvious suppression of infection and great histocompatibility, compared to bare TPU surfaces. We further explored the antimicrobial mechanism of the peptide polymer-modified TPU surfaces, which revealed a surface contact-killing mechanism by disrupting the bacterial membrane. These results demonstrated great potential of the peptide-modified TPU surfaces for practical application to combat bacterial infections that are associated with implantable materials and devices.
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spelling pubmed-81387312021-05-21 Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers Lu, Ziyi Wu, Yueming Cong, Zihao Qian, Yuxin Wu, Xue Shao, Ning Qiao, Zhongqian Zhang, Haodong She, Yunrui Chen, Kang Xiang, Hengxue Sun, Bin Yu, Qian Yuan, Yuan Lin, Haodong Zhu, Meifang Liu, Runhui Bioact Mater Article It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices. Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes. Our recent report on lithium hexamethyldisilazide-initiated superfast polymerization on amino acid N-carboxyanhydrides enables the quick synthesis of host defense peptide-mimicking peptide polymers. Here we reported a facile and cost-effective thermoplastic polyurethane (TPU) surface modification of peptide polymer (DLL: BLG = 90 : 10) using plasma surface activation and substitution reaction between thiol and bromide groups. The peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in vitro. Furthermore, the peptide polymer-modified TPU surfaces showed excellent biocompatibility, displaying no hemolysis and cytotoxicity. In vivo study using methicillin-resistant Staphylococcus aureus (MRSA) for subcutaneous implantation infectious model showed that peptide polymer-modified TPU surfaces revealed obvious suppression of infection and great histocompatibility, compared to bare TPU surfaces. We further explored the antimicrobial mechanism of the peptide polymer-modified TPU surfaces, which revealed a surface contact-killing mechanism by disrupting the bacterial membrane. These results demonstrated great potential of the peptide-modified TPU surfaces for practical application to combat bacterial infections that are associated with implantable materials and devices. KeAi Publishing 2021-05-14 /pmc/articles/PMC8138731/ /pubmed/34027238 http://dx.doi.org/10.1016/j.bioactmat.2021.05.008 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lu, Ziyi
Wu, Yueming
Cong, Zihao
Qian, Yuxin
Wu, Xue
Shao, Ning
Qiao, Zhongqian
Zhang, Haodong
She, Yunrui
Chen, Kang
Xiang, Hengxue
Sun, Bin
Yu, Qian
Yuan, Yuan
Lin, Haodong
Zhu, Meifang
Liu, Runhui
Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers
title Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers
title_full Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers
title_fullStr Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers
title_full_unstemmed Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers
title_short Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers
title_sort effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138731/
https://www.ncbi.nlm.nih.gov/pubmed/34027238
http://dx.doi.org/10.1016/j.bioactmat.2021.05.008
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