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Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers
It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices. Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes. Our recent report on lithium hexamethyldisilazide...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138731/ https://www.ncbi.nlm.nih.gov/pubmed/34027238 http://dx.doi.org/10.1016/j.bioactmat.2021.05.008 |
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author | Lu, Ziyi Wu, Yueming Cong, Zihao Qian, Yuxin Wu, Xue Shao, Ning Qiao, Zhongqian Zhang, Haodong She, Yunrui Chen, Kang Xiang, Hengxue Sun, Bin Yu, Qian Yuan, Yuan Lin, Haodong Zhu, Meifang Liu, Runhui |
author_facet | Lu, Ziyi Wu, Yueming Cong, Zihao Qian, Yuxin Wu, Xue Shao, Ning Qiao, Zhongqian Zhang, Haodong She, Yunrui Chen, Kang Xiang, Hengxue Sun, Bin Yu, Qian Yuan, Yuan Lin, Haodong Zhu, Meifang Liu, Runhui |
author_sort | Lu, Ziyi |
collection | PubMed |
description | It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices. Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes. Our recent report on lithium hexamethyldisilazide-initiated superfast polymerization on amino acid N-carboxyanhydrides enables the quick synthesis of host defense peptide-mimicking peptide polymers. Here we reported a facile and cost-effective thermoplastic polyurethane (TPU) surface modification of peptide polymer (DLL: BLG = 90 : 10) using plasma surface activation and substitution reaction between thiol and bromide groups. The peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in vitro. Furthermore, the peptide polymer-modified TPU surfaces showed excellent biocompatibility, displaying no hemolysis and cytotoxicity. In vivo study using methicillin-resistant Staphylococcus aureus (MRSA) for subcutaneous implantation infectious model showed that peptide polymer-modified TPU surfaces revealed obvious suppression of infection and great histocompatibility, compared to bare TPU surfaces. We further explored the antimicrobial mechanism of the peptide polymer-modified TPU surfaces, which revealed a surface contact-killing mechanism by disrupting the bacterial membrane. These results demonstrated great potential of the peptide-modified TPU surfaces for practical application to combat bacterial infections that are associated with implantable materials and devices. |
format | Online Article Text |
id | pubmed-8138731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-81387312021-05-21 Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers Lu, Ziyi Wu, Yueming Cong, Zihao Qian, Yuxin Wu, Xue Shao, Ning Qiao, Zhongqian Zhang, Haodong She, Yunrui Chen, Kang Xiang, Hengxue Sun, Bin Yu, Qian Yuan, Yuan Lin, Haodong Zhu, Meifang Liu, Runhui Bioact Mater Article It is an urgent need to tackle drug-resistance microbial infections that are associated with implantable biomedical devices. Host defense peptide-mimicking polymers have been actively explored in recent years to fight against drug-resistant microbes. Our recent report on lithium hexamethyldisilazide-initiated superfast polymerization on amino acid N-carboxyanhydrides enables the quick synthesis of host defense peptide-mimicking peptide polymers. Here we reported a facile and cost-effective thermoplastic polyurethane (TPU) surface modification of peptide polymer (DLL: BLG = 90 : 10) using plasma surface activation and substitution reaction between thiol and bromide groups. The peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in vitro. Furthermore, the peptide polymer-modified TPU surfaces showed excellent biocompatibility, displaying no hemolysis and cytotoxicity. In vivo study using methicillin-resistant Staphylococcus aureus (MRSA) for subcutaneous implantation infectious model showed that peptide polymer-modified TPU surfaces revealed obvious suppression of infection and great histocompatibility, compared to bare TPU surfaces. We further explored the antimicrobial mechanism of the peptide polymer-modified TPU surfaces, which revealed a surface contact-killing mechanism by disrupting the bacterial membrane. These results demonstrated great potential of the peptide-modified TPU surfaces for practical application to combat bacterial infections that are associated with implantable materials and devices. KeAi Publishing 2021-05-14 /pmc/articles/PMC8138731/ /pubmed/34027238 http://dx.doi.org/10.1016/j.bioactmat.2021.05.008 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Lu, Ziyi Wu, Yueming Cong, Zihao Qian, Yuxin Wu, Xue Shao, Ning Qiao, Zhongqian Zhang, Haodong She, Yunrui Chen, Kang Xiang, Hengxue Sun, Bin Yu, Qian Yuan, Yuan Lin, Haodong Zhu, Meifang Liu, Runhui Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers |
title | Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers |
title_full | Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers |
title_fullStr | Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers |
title_full_unstemmed | Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers |
title_short | Effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers |
title_sort | effective and biocompatible antibacterial surfaces via facile synthesis and surface modification of peptide polymers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138731/ https://www.ncbi.nlm.nih.gov/pubmed/34027238 http://dx.doi.org/10.1016/j.bioactmat.2021.05.008 |
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