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Isolation of mouse brain-infiltrating leukocytes for single cell profiling of epitopes and transcriptomes
High dimensional compositional and transcriptional profiling of heterogeneous brain-infiltrating leukocytes can lead to novel biological and therapeutic discoveries. High-quality single-cell leukocyte preparations are a prerequisite for optimal single cell profiling. Here, we describe a protocol for...
Autores principales: | Guldner, Ian H., Golomb, Samantha M., Wang, Qingfei, Wang, Emilia, Zhang, Siyuan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138863/ https://www.ncbi.nlm.nih.gov/pubmed/34036283 http://dx.doi.org/10.1016/j.xpro.2021.100537 |
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