Protocol for structural and biochemical analyses of RhoA GTPase

Ras GTPases in complex with Guanosine triphosphate (GTP) or GTP analog exhibit dynamic equilibrium between two interconvertible conformations—an inactive state 1 and an active state 2. Unlike Ras, it remains unclear if the GTP-bound form of Rho GTPases also exhibits multiple conformational states. H...

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Detalles Bibliográficos
Autores principales: Lin, Yuan, Watanabe-Chailland, Miki, Zheng, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138865/
https://www.ncbi.nlm.nih.gov/pubmed/34036285
http://dx.doi.org/10.1016/j.xpro.2021.100541
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author Lin, Yuan
Watanabe-Chailland, Miki
Zheng, Yi
author_facet Lin, Yuan
Watanabe-Chailland, Miki
Zheng, Yi
author_sort Lin, Yuan
collection PubMed
description Ras GTPases in complex with Guanosine triphosphate (GTP) or GTP analog exhibit dynamic equilibrium between two interconvertible conformations—an inactive state 1 and an active state 2. Unlike Ras, it remains unclear if the GTP-bound form of Rho GTPases also exhibits multiple conformational states. Here, we describe a protocol for structural and biochemical analyses of RhoA GTPase. This protocol can be adapted for the characterization of other Rho GTPases. For details on the use and execution of this protocol, please refer to Lin et al. (2021).
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spelling pubmed-81388652021-05-24 Protocol for structural and biochemical analyses of RhoA GTPase Lin, Yuan Watanabe-Chailland, Miki Zheng, Yi STAR Protoc Protocol Ras GTPases in complex with Guanosine triphosphate (GTP) or GTP analog exhibit dynamic equilibrium between two interconvertible conformations—an inactive state 1 and an active state 2. Unlike Ras, it remains unclear if the GTP-bound form of Rho GTPases also exhibits multiple conformational states. Here, we describe a protocol for structural and biochemical analyses of RhoA GTPase. This protocol can be adapted for the characterization of other Rho GTPases. For details on the use and execution of this protocol, please refer to Lin et al. (2021). Elsevier 2021-05-14 /pmc/articles/PMC8138865/ /pubmed/34036285 http://dx.doi.org/10.1016/j.xpro.2021.100541 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lin, Yuan
Watanabe-Chailland, Miki
Zheng, Yi
Protocol for structural and biochemical analyses of RhoA GTPase
title Protocol for structural and biochemical analyses of RhoA GTPase
title_full Protocol for structural and biochemical analyses of RhoA GTPase
title_fullStr Protocol for structural and biochemical analyses of RhoA GTPase
title_full_unstemmed Protocol for structural and biochemical analyses of RhoA GTPase
title_short Protocol for structural and biochemical analyses of RhoA GTPase
title_sort protocol for structural and biochemical analyses of rhoa gtpase
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8138865/
https://www.ncbi.nlm.nih.gov/pubmed/34036285
http://dx.doi.org/10.1016/j.xpro.2021.100541
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