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Genome data of four Pythium insidiosum strains from the phylogenetically-distinct clades I, II, and III

OBJECTIVES: We employed the Illumina NGS platform to sequence genomes of 4 different strains of the pathogenic oomycete Pythium insidiosum, the causative agent of pythiosis. These strains were isolated from humans in Thailand (n=3) and the United States (n=1), and phylogenetically classified into cl...

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Autores principales: Krajaejun, Theerapong, Kittichotirat, Weerayuth, Patumcharoenpol, Preecha, Rujirawat, Thidarat, Lohnoo, Tassanee, Yingyong, Wanta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139067/
https://www.ncbi.nlm.nih.gov/pubmed/34020710
http://dx.doi.org/10.1186/s13104-021-05610-y
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author Krajaejun, Theerapong
Kittichotirat, Weerayuth
Patumcharoenpol, Preecha
Rujirawat, Thidarat
Lohnoo, Tassanee
Yingyong, Wanta
author_facet Krajaejun, Theerapong
Kittichotirat, Weerayuth
Patumcharoenpol, Preecha
Rujirawat, Thidarat
Lohnoo, Tassanee
Yingyong, Wanta
author_sort Krajaejun, Theerapong
collection PubMed
description OBJECTIVES: We employed the Illumina NGS platform to sequence genomes of 4 different strains of the pathogenic oomycete Pythium insidiosum, the causative agent of pythiosis. These strains were isolated from humans in Thailand (n=3) and the United States (n=1), and phylogenetically classified into clade-I, -II, and -III. Our study augmented the completeness of the P. insidiosum genome database for exploration of the biology, evolution, and pathogenesis of the pathogen. DATA DESCRIPTION: One paired-end library (180-bp insert) was prepared from a gDNA sample of P. insidiosum strains ATCC200269 (clade-I), Pi19 (clade-II), MCC18 (clade-II), and SIMI4763 (clade-III) for whole-genome sequencing by Illumina HiSeq2000/HiSeq2500 NGS platform. A range of 28.459.4 million raw reads, accounted for 3.07.3Gb, were obtained and assembled into the genome sizes of 47.1Mb (15,153 contigs; 85% completeness; 19,329 open reading frames [ORFs]) for strain ATCC200269, 35.4Mb (14,576 contigs; 83% completeness; 13,895 ORFs) for strain Pi19, 34.5Mb (11,084 contigs; 84% completeness; 13,249 ORFs) for strain MCC18, and 47.1Mb (15,162 contigs; 85% completeness; 19,340 ORFs) for strain SIMI4763. The genome data can be downloaded from the NCBI/DDBJ databases under the accessions BCFN00000000.1 (ATCC200269), BCFS00000000.1 (Pi19), BCFT00000000.1 (MCC18), and BCFU00000000.1 (SIMI4763).
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spelling pubmed-81390672021-05-21 Genome data of four Pythium insidiosum strains from the phylogenetically-distinct clades I, II, and III Krajaejun, Theerapong Kittichotirat, Weerayuth Patumcharoenpol, Preecha Rujirawat, Thidarat Lohnoo, Tassanee Yingyong, Wanta BMC Res Notes Data Note OBJECTIVES: We employed the Illumina NGS platform to sequence genomes of 4 different strains of the pathogenic oomycete Pythium insidiosum, the causative agent of pythiosis. These strains were isolated from humans in Thailand (n=3) and the United States (n=1), and phylogenetically classified into clade-I, -II, and -III. Our study augmented the completeness of the P. insidiosum genome database for exploration of the biology, evolution, and pathogenesis of the pathogen. DATA DESCRIPTION: One paired-end library (180-bp insert) was prepared from a gDNA sample of P. insidiosum strains ATCC200269 (clade-I), Pi19 (clade-II), MCC18 (clade-II), and SIMI4763 (clade-III) for whole-genome sequencing by Illumina HiSeq2000/HiSeq2500 NGS platform. A range of 28.459.4 million raw reads, accounted for 3.07.3Gb, were obtained and assembled into the genome sizes of 47.1Mb (15,153 contigs; 85% completeness; 19,329 open reading frames [ORFs]) for strain ATCC200269, 35.4Mb (14,576 contigs; 83% completeness; 13,895 ORFs) for strain Pi19, 34.5Mb (11,084 contigs; 84% completeness; 13,249 ORFs) for strain MCC18, and 47.1Mb (15,162 contigs; 85% completeness; 19,340 ORFs) for strain SIMI4763. The genome data can be downloaded from the NCBI/DDBJ databases under the accessions BCFN00000000.1 (ATCC200269), BCFS00000000.1 (Pi19), BCFT00000000.1 (MCC18), and BCFU00000000.1 (SIMI4763). BioMed Central 2021-05-21 /pmc/articles/PMC8139067/ /pubmed/34020710 http://dx.doi.org/10.1186/s13104-021-05610-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Data Note
Krajaejun, Theerapong
Kittichotirat, Weerayuth
Patumcharoenpol, Preecha
Rujirawat, Thidarat
Lohnoo, Tassanee
Yingyong, Wanta
Genome data of four Pythium insidiosum strains from the phylogenetically-distinct clades I, II, and III
title Genome data of four Pythium insidiosum strains from the phylogenetically-distinct clades I, II, and III
title_full Genome data of four Pythium insidiosum strains from the phylogenetically-distinct clades I, II, and III
title_fullStr Genome data of four Pythium insidiosum strains from the phylogenetically-distinct clades I, II, and III
title_full_unstemmed Genome data of four Pythium insidiosum strains from the phylogenetically-distinct clades I, II, and III
title_short Genome data of four Pythium insidiosum strains from the phylogenetically-distinct clades I, II, and III
title_sort genome data of four pythium insidiosum strains from the phylogenetically-distinct clades i, ii, and iii
topic Data Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139067/
https://www.ncbi.nlm.nih.gov/pubmed/34020710
http://dx.doi.org/10.1186/s13104-021-05610-y
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