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The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis

The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process similar to that in the human outer subventricular zone. We perform targeted single-cell mRNA sequencing in third instar larval brains to study...

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Autores principales: Michki, Nigel S., Li, Ye, Sanjasaz, Kayvon, Zhao, Yimeng, Shen, Fred Y., Walker, Logan A., Cao, Wenjia, Lee, Cheng-Yu, Cai, Dawen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139287/
https://www.ncbi.nlm.nih.gov/pubmed/33909998
http://dx.doi.org/10.1016/j.celrep.2021.109039
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author Michki, Nigel S.
Li, Ye
Sanjasaz, Kayvon
Zhao, Yimeng
Shen, Fred Y.
Walker, Logan A.
Cao, Wenjia
Lee, Cheng-Yu
Cai, Dawen
author_facet Michki, Nigel S.
Li, Ye
Sanjasaz, Kayvon
Zhao, Yimeng
Shen, Fred Y.
Walker, Logan A.
Cao, Wenjia
Lee, Cheng-Yu
Cai, Dawen
author_sort Michki, Nigel S.
collection PubMed
description The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process similar to that in the human outer subventricular zone. We perform targeted single-cell mRNA sequencing in third instar larval brains to study this process of the type II NB lineage. Combining prior knowledge, in silico analyses, and in situ validation, our multi-informatic investigation describes the molecular landscape from a single developmental snapshot. 17 markers are identified to differentiate distinct maturation stages. 30 markers are identified to specify the stem cell origin and/or cell division numbers of INPs, and at least 12 neuronal subtypes are identified. To foster future discoveries, we provide annotated tables of pairwise gene-gene correlation in single cells and MiCV, a web tool for interactively analyzing scRNA-seq datasets. Taken together, these resources advance our understanding of the neural differentiation process at the molecular level.
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spelling pubmed-81392872021-05-21 The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis Michki, Nigel S. Li, Ye Sanjasaz, Kayvon Zhao, Yimeng Shen, Fred Y. Walker, Logan A. Cao, Wenjia Lee, Cheng-Yu Cai, Dawen Cell Rep Article The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process similar to that in the human outer subventricular zone. We perform targeted single-cell mRNA sequencing in third instar larval brains to study this process of the type II NB lineage. Combining prior knowledge, in silico analyses, and in situ validation, our multi-informatic investigation describes the molecular landscape from a single developmental snapshot. 17 markers are identified to differentiate distinct maturation stages. 30 markers are identified to specify the stem cell origin and/or cell division numbers of INPs, and at least 12 neuronal subtypes are identified. To foster future discoveries, we provide annotated tables of pairwise gene-gene correlation in single cells and MiCV, a web tool for interactively analyzing scRNA-seq datasets. Taken together, these resources advance our understanding of the neural differentiation process at the molecular level. 2021-04-27 /pmc/articles/PMC8139287/ /pubmed/33909998 http://dx.doi.org/10.1016/j.celrep.2021.109039 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Michki, Nigel S.
Li, Ye
Sanjasaz, Kayvon
Zhao, Yimeng
Shen, Fred Y.
Walker, Logan A.
Cao, Wenjia
Lee, Cheng-Yu
Cai, Dawen
The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis
title The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis
title_full The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis
title_fullStr The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis
title_full_unstemmed The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis
title_short The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis
title_sort molecular landscape of neural differentiation in the developing drosophila brain revealed by targeted scrna-seq and multi-informatic analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139287/
https://www.ncbi.nlm.nih.gov/pubmed/33909998
http://dx.doi.org/10.1016/j.celrep.2021.109039
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