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The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis
The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process similar to that in the human outer subventricular zone. We perform targeted single-cell mRNA sequencing in third instar larval brains to study...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139287/ https://www.ncbi.nlm.nih.gov/pubmed/33909998 http://dx.doi.org/10.1016/j.celrep.2021.109039 |
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author | Michki, Nigel S. Li, Ye Sanjasaz, Kayvon Zhao, Yimeng Shen, Fred Y. Walker, Logan A. Cao, Wenjia Lee, Cheng-Yu Cai, Dawen |
author_facet | Michki, Nigel S. Li, Ye Sanjasaz, Kayvon Zhao, Yimeng Shen, Fred Y. Walker, Logan A. Cao, Wenjia Lee, Cheng-Yu Cai, Dawen |
author_sort | Michki, Nigel S. |
collection | PubMed |
description | The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process similar to that in the human outer subventricular zone. We perform targeted single-cell mRNA sequencing in third instar larval brains to study this process of the type II NB lineage. Combining prior knowledge, in silico analyses, and in situ validation, our multi-informatic investigation describes the molecular landscape from a single developmental snapshot. 17 markers are identified to differentiate distinct maturation stages. 30 markers are identified to specify the stem cell origin and/or cell division numbers of INPs, and at least 12 neuronal subtypes are identified. To foster future discoveries, we provide annotated tables of pairwise gene-gene correlation in single cells and MiCV, a web tool for interactively analyzing scRNA-seq datasets. Taken together, these resources advance our understanding of the neural differentiation process at the molecular level. |
format | Online Article Text |
id | pubmed-8139287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-81392872021-05-21 The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis Michki, Nigel S. Li, Ye Sanjasaz, Kayvon Zhao, Yimeng Shen, Fred Y. Walker, Logan A. Cao, Wenjia Lee, Cheng-Yu Cai, Dawen Cell Rep Article The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process similar to that in the human outer subventricular zone. We perform targeted single-cell mRNA sequencing in third instar larval brains to study this process of the type II NB lineage. Combining prior knowledge, in silico analyses, and in situ validation, our multi-informatic investigation describes the molecular landscape from a single developmental snapshot. 17 markers are identified to differentiate distinct maturation stages. 30 markers are identified to specify the stem cell origin and/or cell division numbers of INPs, and at least 12 neuronal subtypes are identified. To foster future discoveries, we provide annotated tables of pairwise gene-gene correlation in single cells and MiCV, a web tool for interactively analyzing scRNA-seq datasets. Taken together, these resources advance our understanding of the neural differentiation process at the molecular level. 2021-04-27 /pmc/articles/PMC8139287/ /pubmed/33909998 http://dx.doi.org/10.1016/j.celrep.2021.109039 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ). |
spellingShingle | Article Michki, Nigel S. Li, Ye Sanjasaz, Kayvon Zhao, Yimeng Shen, Fred Y. Walker, Logan A. Cao, Wenjia Lee, Cheng-Yu Cai, Dawen The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis |
title | The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis |
title_full | The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis |
title_fullStr | The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis |
title_full_unstemmed | The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis |
title_short | The molecular landscape of neural differentiation in the developing Drosophila brain revealed by targeted scRNA-seq and multi-informatic analysis |
title_sort | molecular landscape of neural differentiation in the developing drosophila brain revealed by targeted scrna-seq and multi-informatic analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139287/ https://www.ncbi.nlm.nih.gov/pubmed/33909998 http://dx.doi.org/10.1016/j.celrep.2021.109039 |
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