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Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine
The prediction of the fertilizing ability of a seminal dose continues to be a primary aim in the field of artificial insemination (AI). To achieve this goal, in this study we have included the evaluation of some non-conventional sperm quality markers. A total of 3,906 ewes from 52 different farms we...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139619/ https://www.ncbi.nlm.nih.gov/pubmed/34026889 http://dx.doi.org/10.3389/fvets.2021.650572 |
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author | Mendoza, Noelia Casao, Adriana Domingo, Juan Quintín, Francisco Laviña, Adolfo Fantova, Enrique Cebrián-Pérez, José Álvaro Muiño-Blanco, Teresa Pérez-Pe, Rosaura |
author_facet | Mendoza, Noelia Casao, Adriana Domingo, Juan Quintín, Francisco Laviña, Adolfo Fantova, Enrique Cebrián-Pérez, José Álvaro Muiño-Blanco, Teresa Pérez-Pe, Rosaura |
author_sort | Mendoza, Noelia |
collection | PubMed |
description | The prediction of the fertilizing ability of a seminal dose continues to be a primary aim in the field of artificial insemination (AI). To achieve this goal, in this study we have included the evaluation of some non-conventional sperm quality markers. A total of 3,906 ewes from 52 different farms were inseminated with 357 refrigerated seminal doses obtained from 45 mature Rasa Aragonesa rams. The same samples were used for sperm quality analysis including membrane integrity, capacitation status, oxygen consumption and apoptotic-like markers such as phosphatidylserine translocation (PS), plasmalemma disorganization/mitochondrial membrane potential, caspase activation and DNA damage. Seminal doses from the breeding (B) season presented higher percentages of intact membrane (IM), non permeant (NP) membrane with high mitochondrial membrane potential (ΔΨm) and IM without PS translocation spermatozoa than those from the non-breeding (NB) season. Therefore, we can conclude that there were less spermatozoa showing apoptotic-like features in the seminal doses from the B than the NB season, although these differences did not affect field fertility. Only the percentage of intact membrane, non-capacitated (IM-NC) spermatozoa showed a significant correlation with in vivo fertility (P = 0.005) and fecundity (P = 0.007) values obtained after cervical AI when all data were evaluated. When the data were sorted by season and distance to the farms where AI was performed, the correlation between the percentage of IM-NC spermatozoa and reproductive parameters increased in the NB season and progressively with remoteness from the farms. Some other sperm parameters, like NP with high ΔΨm, IM sperm without active caspases and DNA-intact spermatozoa, also showed significant correlations with the reproductive parameters in the sorted data. Moreover, the increment in both the percentage of IM-NC and DNA-intact spermatozoa would increase the probability of obtaining a fertility higher than the mean (>52%), as revealed by a multiple logistic regression analysis. In conclusion, we have identified two seminal markers—the percentage of intact membrane, non-capacitated spermatozoa, and DNA intact spermatozoa—which could be used as a test to discard males in AI programs, which is highly important from an economic point of view and can contribute to achieving satisfactory fertility rates. |
format | Online Article Text |
id | pubmed-8139619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81396192021-05-22 Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine Mendoza, Noelia Casao, Adriana Domingo, Juan Quintín, Francisco Laviña, Adolfo Fantova, Enrique Cebrián-Pérez, José Álvaro Muiño-Blanco, Teresa Pérez-Pe, Rosaura Front Vet Sci Veterinary Science The prediction of the fertilizing ability of a seminal dose continues to be a primary aim in the field of artificial insemination (AI). To achieve this goal, in this study we have included the evaluation of some non-conventional sperm quality markers. A total of 3,906 ewes from 52 different farms were inseminated with 357 refrigerated seminal doses obtained from 45 mature Rasa Aragonesa rams. The same samples were used for sperm quality analysis including membrane integrity, capacitation status, oxygen consumption and apoptotic-like markers such as phosphatidylserine translocation (PS), plasmalemma disorganization/mitochondrial membrane potential, caspase activation and DNA damage. Seminal doses from the breeding (B) season presented higher percentages of intact membrane (IM), non permeant (NP) membrane with high mitochondrial membrane potential (ΔΨm) and IM without PS translocation spermatozoa than those from the non-breeding (NB) season. Therefore, we can conclude that there were less spermatozoa showing apoptotic-like features in the seminal doses from the B than the NB season, although these differences did not affect field fertility. Only the percentage of intact membrane, non-capacitated (IM-NC) spermatozoa showed a significant correlation with in vivo fertility (P = 0.005) and fecundity (P = 0.007) values obtained after cervical AI when all data were evaluated. When the data were sorted by season and distance to the farms where AI was performed, the correlation between the percentage of IM-NC spermatozoa and reproductive parameters increased in the NB season and progressively with remoteness from the farms. Some other sperm parameters, like NP with high ΔΨm, IM sperm without active caspases and DNA-intact spermatozoa, also showed significant correlations with the reproductive parameters in the sorted data. Moreover, the increment in both the percentage of IM-NC and DNA-intact spermatozoa would increase the probability of obtaining a fertility higher than the mean (>52%), as revealed by a multiple logistic regression analysis. In conclusion, we have identified two seminal markers—the percentage of intact membrane, non-capacitated spermatozoa, and DNA intact spermatozoa—which could be used as a test to discard males in AI programs, which is highly important from an economic point of view and can contribute to achieving satisfactory fertility rates. Frontiers Media S.A. 2021-05-07 /pmc/articles/PMC8139619/ /pubmed/34026889 http://dx.doi.org/10.3389/fvets.2021.650572 Text en Copyright © 2021 Mendoza, Casao, Domingo, Quintín, Laviña, Fantova, Cebrián-Pérez, Muiño-Blanco and Pérez-Pe. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Mendoza, Noelia Casao, Adriana Domingo, Juan Quintín, Francisco Laviña, Adolfo Fantova, Enrique Cebrián-Pérez, José Álvaro Muiño-Blanco, Teresa Pérez-Pe, Rosaura Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine |
title | Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine |
title_full | Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine |
title_fullStr | Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine |
title_full_unstemmed | Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine |
title_short | Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine |
title_sort | influence of non-conventional sperm quality parameters on field fertility in ovine |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139619/ https://www.ncbi.nlm.nih.gov/pubmed/34026889 http://dx.doi.org/10.3389/fvets.2021.650572 |
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