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The Association Between β-Dystroglycan in Airway Smooth Muscle and Eosinophils in Allergic Asthma
Allergic asthma (AA) is a complex disorder with heterogeneous features of airway hyperresponsiveness, inflammation, and remodeling. The increase of airway smooth muscle (ASM) mass is a fundamental component of bronchial remodeling in AA, yet the pathophysiological mechanisms and clinical outcomes as...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139938/ https://www.ncbi.nlm.nih.gov/pubmed/33566255 http://dx.doi.org/10.1007/s10753-020-01401-y |
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author | Shareef, Suhayla H. Amin, Kawa Janson, Christer |
author_facet | Shareef, Suhayla H. Amin, Kawa Janson, Christer |
author_sort | Shareef, Suhayla H. |
collection | PubMed |
description | Allergic asthma (AA) is a complex disorder with heterogeneous features of airway hyperresponsiveness, inflammation, and remodeling. The increase of airway smooth muscle (ASM) mass is a fundamental component of bronchial remodeling in AA, yet the pathophysiological mechanisms and clinical outcomes associated with ASM modulation are still elusive. The objective of this study is to compare the expression level of β-dystroglycan (β-DG) in ASM in AA subjects and a healthy control group and to investigate the relationship between eosinophils and β-DG in ASM in patients with AA. Thirteen AA patients and seven control subjects were analyzed for the ASM area and eosinophil cells. Bronchial biopsies were stained by β-DG and eosinophil cationic protein (ECP) using immunohistochemistry. The proportion of ASM with β-DG staining was greater in those with AA than in the healthy control group (mean (95% CI) (28.3% (23.8–32.7%) vs. 16.4% (14.1–18.5%), P < 0.0001). The number of ECP positive cells was higher in patients with AA than in the control group (4056 (3819–4296) vs. 466 (395–537) cells/mm(2) P < 0.0001). In AA, the number of ECP positive cells was significantly correlated to the β-DG expression in ASM (r = 0.77, P = 0.002). There is an increased β-DG expression in ASM and a higher number of ECP positive cells in the bronchial biopsy of those with AA than those in the control group. The increased expression of β-DG in ASM in AA subjects correlates with the number of eosinophils, suggesting a role for this cell in airway remodeling in AA. |
format | Online Article Text |
id | pubmed-8139938 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-81399382021-06-03 The Association Between β-Dystroglycan in Airway Smooth Muscle and Eosinophils in Allergic Asthma Shareef, Suhayla H. Amin, Kawa Janson, Christer Inflammation Original Article Allergic asthma (AA) is a complex disorder with heterogeneous features of airway hyperresponsiveness, inflammation, and remodeling. The increase of airway smooth muscle (ASM) mass is a fundamental component of bronchial remodeling in AA, yet the pathophysiological mechanisms and clinical outcomes associated with ASM modulation are still elusive. The objective of this study is to compare the expression level of β-dystroglycan (β-DG) in ASM in AA subjects and a healthy control group and to investigate the relationship between eosinophils and β-DG in ASM in patients with AA. Thirteen AA patients and seven control subjects were analyzed for the ASM area and eosinophil cells. Bronchial biopsies were stained by β-DG and eosinophil cationic protein (ECP) using immunohistochemistry. The proportion of ASM with β-DG staining was greater in those with AA than in the healthy control group (mean (95% CI) (28.3% (23.8–32.7%) vs. 16.4% (14.1–18.5%), P < 0.0001). The number of ECP positive cells was higher in patients with AA than in the control group (4056 (3819–4296) vs. 466 (395–537) cells/mm(2) P < 0.0001). In AA, the number of ECP positive cells was significantly correlated to the β-DG expression in ASM (r = 0.77, P = 0.002). There is an increased β-DG expression in ASM and a higher number of ECP positive cells in the bronchial biopsy of those with AA than those in the control group. The increased expression of β-DG in ASM in AA subjects correlates with the number of eosinophils, suggesting a role for this cell in airway remodeling in AA. Springer US 2021-02-10 2021 /pmc/articles/PMC8139938/ /pubmed/33566255 http://dx.doi.org/10.1007/s10753-020-01401-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Shareef, Suhayla H. Amin, Kawa Janson, Christer The Association Between β-Dystroglycan in Airway Smooth Muscle and Eosinophils in Allergic Asthma |
title | The Association Between β-Dystroglycan in Airway Smooth Muscle and Eosinophils in Allergic Asthma |
title_full | The Association Between β-Dystroglycan in Airway Smooth Muscle and Eosinophils in Allergic Asthma |
title_fullStr | The Association Between β-Dystroglycan in Airway Smooth Muscle and Eosinophils in Allergic Asthma |
title_full_unstemmed | The Association Between β-Dystroglycan in Airway Smooth Muscle and Eosinophils in Allergic Asthma |
title_short | The Association Between β-Dystroglycan in Airway Smooth Muscle and Eosinophils in Allergic Asthma |
title_sort | association between β-dystroglycan in airway smooth muscle and eosinophils in allergic asthma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139938/ https://www.ncbi.nlm.nih.gov/pubmed/33566255 http://dx.doi.org/10.1007/s10753-020-01401-y |
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