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Chemically defined and xeno-free culture condition for human extended pluripotent stem cells
Extended pluripotent stem (EPS) cells have shown great applicative potentials in generating synthetic embryos, directed differentiation and disease modeling. However, the lack of a xeno-free culture condition has significantly limited their applications. Here, we report a chemically defined and xeno...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139978/ https://www.ncbi.nlm.nih.gov/pubmed/34021145 http://dx.doi.org/10.1038/s41467-021-23320-8 |
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author | Liu, Bei Chen, Shi Xu, Yaxing Lyu, Yulin Wang, Jinlin Du, Yuanyuan Sun, Yongcheng Liu, Heming Zhou, Haoying Lai, Weifeng Xue, Anqi Yin, Ming Li, Cheng Bai, Yun Xu, Jun Deng, Hongkui |
author_facet | Liu, Bei Chen, Shi Xu, Yaxing Lyu, Yulin Wang, Jinlin Du, Yuanyuan Sun, Yongcheng Liu, Heming Zhou, Haoying Lai, Weifeng Xue, Anqi Yin, Ming Li, Cheng Bai, Yun Xu, Jun Deng, Hongkui |
author_sort | Liu, Bei |
collection | PubMed |
description | Extended pluripotent stem (EPS) cells have shown great applicative potentials in generating synthetic embryos, directed differentiation and disease modeling. However, the lack of a xeno-free culture condition has significantly limited their applications. Here, we report a chemically defined and xeno-free culture system for culturing and deriving human EPS cells in vitro. Xeno-free human EPS cells can be long-term and genetically stably maintained in vitro, as well as preserve their embryonic and extraembryonic developmental potentials. Furthermore, the xeno-free culturing system also permits efficient derivation of human EPS cells from human fibroblast through reprogramming. Our study could have broad utility in future applications of human EPS cells in biomedicine. |
format | Online Article Text |
id | pubmed-8139978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81399782021-06-03 Chemically defined and xeno-free culture condition for human extended pluripotent stem cells Liu, Bei Chen, Shi Xu, Yaxing Lyu, Yulin Wang, Jinlin Du, Yuanyuan Sun, Yongcheng Liu, Heming Zhou, Haoying Lai, Weifeng Xue, Anqi Yin, Ming Li, Cheng Bai, Yun Xu, Jun Deng, Hongkui Nat Commun Article Extended pluripotent stem (EPS) cells have shown great applicative potentials in generating synthetic embryos, directed differentiation and disease modeling. However, the lack of a xeno-free culture condition has significantly limited their applications. Here, we report a chemically defined and xeno-free culture system for culturing and deriving human EPS cells in vitro. Xeno-free human EPS cells can be long-term and genetically stably maintained in vitro, as well as preserve their embryonic and extraembryonic developmental potentials. Furthermore, the xeno-free culturing system also permits efficient derivation of human EPS cells from human fibroblast through reprogramming. Our study could have broad utility in future applications of human EPS cells in biomedicine. Nature Publishing Group UK 2021-05-21 /pmc/articles/PMC8139978/ /pubmed/34021145 http://dx.doi.org/10.1038/s41467-021-23320-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Liu, Bei Chen, Shi Xu, Yaxing Lyu, Yulin Wang, Jinlin Du, Yuanyuan Sun, Yongcheng Liu, Heming Zhou, Haoying Lai, Weifeng Xue, Anqi Yin, Ming Li, Cheng Bai, Yun Xu, Jun Deng, Hongkui Chemically defined and xeno-free culture condition for human extended pluripotent stem cells |
title | Chemically defined and xeno-free culture condition for human extended pluripotent stem cells |
title_full | Chemically defined and xeno-free culture condition for human extended pluripotent stem cells |
title_fullStr | Chemically defined and xeno-free culture condition for human extended pluripotent stem cells |
title_full_unstemmed | Chemically defined and xeno-free culture condition for human extended pluripotent stem cells |
title_short | Chemically defined and xeno-free culture condition for human extended pluripotent stem cells |
title_sort | chemically defined and xeno-free culture condition for human extended pluripotent stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8139978/ https://www.ncbi.nlm.nih.gov/pubmed/34021145 http://dx.doi.org/10.1038/s41467-021-23320-8 |
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