Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection

SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent...

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Autores principales: Piñero, Paula, Marco De La Calle, Francisco M, Horndler, Lydia, Alarcón, Balbino, Uribe Barrientos, Marisol, Sarmiento, Héctor, Tarín, Fabián
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140089/
https://www.ncbi.nlm.nih.gov/pubmed/34021205
http://dx.doi.org/10.1038/s41598-021-90054-4
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author Piñero, Paula
Marco De La Calle, Francisco M
Horndler, Lydia
Alarcón, Balbino
Uribe Barrientos, Marisol
Sarmiento, Héctor
Tarín, Fabián
author_facet Piñero, Paula
Marco De La Calle, Francisco M
Horndler, Lydia
Alarcón, Balbino
Uribe Barrientos, Marisol
Sarmiento, Héctor
Tarín, Fabián
author_sort Piñero, Paula
collection PubMed
description SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent a key tool. Classic serological tests, based on colorimetric techniques, such as ELISA or CLIA, continue to be the most widely used option. However, a real improvement in results is still needed. We developed a highly sensitive and specific FCM assay that allows the detection of IgG and IgA antibodies, directed against the native and functional S-protein of SARS-CoV-2 exposed on the membrane of a transfected cell line, up to 8 months after infection.
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spelling pubmed-81400892021-05-25 Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection Piñero, Paula Marco De La Calle, Francisco M Horndler, Lydia Alarcón, Balbino Uribe Barrientos, Marisol Sarmiento, Héctor Tarín, Fabián Sci Rep Article SARS-CoV-2 is the virus that causes the disease called COVID-19, which has caused the worst pandemic of the century. Both, to know the immunological status of general population and to evaluate the efficacy of the vaccination process that is taking place around the world, serological tests represent a key tool. Classic serological tests, based on colorimetric techniques, such as ELISA or CLIA, continue to be the most widely used option. However, a real improvement in results is still needed. We developed a highly sensitive and specific FCM assay that allows the detection of IgG and IgA antibodies, directed against the native and functional S-protein of SARS-CoV-2 exposed on the membrane of a transfected cell line, up to 8 months after infection. Nature Publishing Group UK 2021-05-21 /pmc/articles/PMC8140089/ /pubmed/34021205 http://dx.doi.org/10.1038/s41598-021-90054-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If mle 5aterial is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Piñero, Paula
Marco De La Calle, Francisco M
Horndler, Lydia
Alarcón, Balbino
Uribe Barrientos, Marisol
Sarmiento, Héctor
Tarín, Fabián
Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection
title Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection
title_full Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection
title_fullStr Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection
title_full_unstemmed Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection
title_short Flow cytometry detection of sustained humoral immune response (IgG + IgA) against native spike glycoprotein in asymptomatic/mild SARS-CoV-2 infection
title_sort flow cytometry detection of sustained humoral immune response (igg + iga) against native spike glycoprotein in asymptomatic/mild sars-cov-2 infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140089/
https://www.ncbi.nlm.nih.gov/pubmed/34021205
http://dx.doi.org/10.1038/s41598-021-90054-4
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