Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution

Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develo...

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Autores principales: Berardi, Massimiliano, Bielawski, Kevin, Rijnveld, Niek, Gruca, Grzegorz, Aardema, Hilde, van Tol, Leni, Wuite, Gijs, Akca, B. Imran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140111/
https://www.ncbi.nlm.nih.gov/pubmed/34021241
http://dx.doi.org/10.1038/s42003-021-02121-1
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author Berardi, Massimiliano
Bielawski, Kevin
Rijnveld, Niek
Gruca, Grzegorz
Aardema, Hilde
van Tol, Leni
Wuite, Gijs
Akca, B. Imran
author_facet Berardi, Massimiliano
Bielawski, Kevin
Rijnveld, Niek
Gruca, Grzegorz
Aardema, Hilde
van Tol, Leni
Wuite, Gijs
Akca, B. Imran
author_sort Berardi, Massimiliano
collection PubMed
description Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develop a MPA system that measures pressure and displacement in real time with sub-nanometer resolution thanks to an interferometric readout. This highly sensitive MPA system enables studying the nanoscale behavior of soft biomaterials under tension and their frequency-dependent viscoelastic response.
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spelling pubmed-81401112021-06-03 Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution Berardi, Massimiliano Bielawski, Kevin Rijnveld, Niek Gruca, Grzegorz Aardema, Hilde van Tol, Leni Wuite, Gijs Akca, B. Imran Commun Biol Article Micropipette aspiration (MPA) is an essential tool in mechanobiology; however, its potential is far from fully exploited. The traditional MPA technique has limited temporal and spatial resolution and requires extensive post processing to obtain the mechanical fingerprints of samples. Here, we develop a MPA system that measures pressure and displacement in real time with sub-nanometer resolution thanks to an interferometric readout. This highly sensitive MPA system enables studying the nanoscale behavior of soft biomaterials under tension and their frequency-dependent viscoelastic response. Nature Publishing Group UK 2021-05-21 /pmc/articles/PMC8140111/ /pubmed/34021241 http://dx.doi.org/10.1038/s42003-021-02121-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Berardi, Massimiliano
Bielawski, Kevin
Rijnveld, Niek
Gruca, Grzegorz
Aardema, Hilde
van Tol, Leni
Wuite, Gijs
Akca, B. Imran
Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution
title Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution
title_full Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution
title_fullStr Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution
title_full_unstemmed Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution
title_short Optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution
title_sort optical interferometry based micropipette aspiration provides real-time sub-nanometer spatial resolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8140111/
https://www.ncbi.nlm.nih.gov/pubmed/34021241
http://dx.doi.org/10.1038/s42003-021-02121-1
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